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AB323717

Anti-MARCO antibody [RM1219]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • 20ul selling size
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Rabbit Recombinant Multiclonal MARCO antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

View Alternative Names

Macrophage receptor MARCO, Macrophage receptor with collagenous structure, Marco, SCARA2, MARCO, Macrophage receptor MARCO, Macrophage receptor with collagenous structure, Scavenger receptor class A member 2

22 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on the Kupffer cells in human liver. The section was incubated with ab323717 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining in human pancreas. The section was incubated with ab323717 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [RM1219] (AB323717)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [RM1219] (AB323717)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labelling MARCO with ab323717 at 1/500 (0.996 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing the cytoplasmic and membranous staining is increased in subsets of human PBMCs (shown in green) treated with 50 ng/ml M-CSF for 72 hours, then add 20 ng/ml IL-10 for 24 hours. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab213363 anti-CD68 used at 1 : 500 (1.26 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD68 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human liver.
Panel B : anti-MARCO staining macrophages in human liver.
Panel C : ant-CD68 staining macrophages in human liver.
Panel D : ant-CD3 staining T lymphocytes in human liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab213363 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on the macrophages in human lung. The section was incubated with ab323717 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab320735 anti-CD19 used at 1 : 2000 (0.255 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™570) and anti-CD3 (magenta; Opal™690) on human liver.
Panel B : anti-MARCO staining macrophages in human liver.
Panel C : ant-CD19 staining B lymphocytes in human liver.
Panel D : ant-CD3 staining T lymphocytes in human liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab320735 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spleen tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab245235 anti-CD19 used at 1 : 1000 (0.22 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on mouse spleen.
Panel B : anti-MARCO staining macrophages in mouse spleen.
Panel C : ant-CD19 staining B lymphocytes in mouse spleen.
Panel D : ant-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab245235 anti-CD19 used at 1 : 1000 (0.22 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on mouse liver.
Panel B : anti-MARCO staining macrophages in mouse liver.
Panel C : ant-CD19 staining B lymphocytes in mouse liver.
Panel D : ant-CD3 staining T lymphocytes in mouse liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [RM1219] (AB323717)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [RM1219] (AB323717)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized J774A.1(mouse reticulum cell sarcoma monocyte/macrophage) cells labelling MARCO with ab323717 at 1/500 (0.996 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing increased cytoplasmic staining in J774A.1 cells treated with 1 ng/ml lipopolysaccharide (LPS) for 24 hours (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling MARCO with ab323717 at 1/400 (1.245 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Positive staining on the Kupffer cells in rat liver. The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling MARCO with ab323717 at 1/400 (1.245 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Positive staining on the macrophages in rat lung. The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on the Kupffer cells in mouse liver. The section was incubated with ab323717 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab245235 anti-CD19 used at 1 : 1000 (0.22 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on mouse lung.
Panel B : anti-MARCO staining macrophages in mouse lung.
Panel C : ant-CD19 staining B lymphocytes in mouse lung.
Panel D : ant-CD3 staining T lymphocytes in mouse lung.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on the macrophages in mouse lung. The section was incubated with ab323717 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab283654 anti-CD68 used at 1 : 100 (4.43 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD68 (magenta; Opal™690) and anti-CD3 (yellow; Opal™570) on rat liver.
Panel B : anti-MARCO staining macrophages in rat liver.
Panel C : ant-CD68 staining macrophages in rat liver.
Panel D : ant-CD3 staining T lymphocytes in rat liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab283654 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab283654 anti-CD68 used at 1 : 100 (4.43 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD68 (magenta; Opal™690) and anti-CD3 (yellow; Opal™570) on mouse lung.
Panel B : anti-MARCO staining macrophages in mouse lung.
Panel C : ant-CD68 staining macrophages in mouse lung.
Panel D : ant-CD3 staining T lymphocytes in mouse lung.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab283654 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining in mouse pancreas. The section was incubated with ab322705 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling MARCO with ab323717 at 1/400 (1.245 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Negative control : no staining in rat pancreas. The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab64088 anti-CD20 used at 1 : 100 (2.91 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD20 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on rat liver.
Panel B : anti-MARCO staining macrophages in rat liver.
Panel C : ant-CD20 staining B lymphocytes in rat liver.
Panel D : ant-CD3 staining T lymphocytes in rat liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab64088 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Western blot - Anti-MARCO antibody [RM1219] (AB323717)
  • WB

Supplier Data

Western blot - Anti-MARCO antibody [RM1219] (AB323717)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : pancreas.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MARCO antibody [RM1219] (ab323717) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 40 µg

Lane 2:

Human pancreas tissue lysate at 40 µg

Lane 3:

Human lung tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-MARCO antibody [RM1219] (AB323717)
  • WB

Supplier Data

Western blot - Anti-MARCO antibody [RM1219] (AB323717)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 26892079).

The expression of MARCO/Macrophage receptor is induced by Lipopolysaccharides (LPS) treatment (PMID : 9916718).

In Western blot loading control Anti-Vinculin antibody [EPR8185] (ab129002)(1/5000)(124KDa).

All lanes:

Western blot - Anti-MARCO antibody [RM1219] (ab323717) at 1/1000 dilution

Lane 1:

Untreated J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ), whole cell lysate at 40 µg

Lane 2:

J774A.1 treated with 1ug/ml Lipopolysaccharide (LPS) and 300ng/ml 24h Brefeldin A (BFA) for 24 hours, whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 60-75 kDa,124 kDa

false

Exposure time: 180s

Western blot - Anti-MARCO antibody [RM1219] (AB323717)
  • WB

Supplier Data

Western blot - Anti-MARCO antibody [RM1219] (AB323717)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : pancreas, skeletal muscle.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MARCO antibody [RM1219] (ab323717) at 1/1000 dilution

Lane 1:

Mouse lymph node tissue lysate at 20 µg

Lane 2:

Mouse pancreas tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 4:

Rat lymph node tissue lysate at 5 µg

Lane 5:

Rat skeletal muscle tissue lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa

false

Exposure time: 59s

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1219

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Pattern recognition receptor (PRR) which binds Gram-positive and Gram-negative bacteria (PubMed : 7867067). Also plays a role in binding of unopsonized particles by alveolar macrophages (By similarity). Binds to the secretoglobin SCGB3A2 (By similarity).
See full target information Marco

Additional targets

MARCO,Macrophage receptor MARCO

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