Rabbit Polyclonal MARK1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with African green monkey, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MARK1.
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Publications
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- Endocrinology 163:2022MARK2 and MARK4 Regulate Sertoli Cell BTB Dynamics Through Microtubule and Actin Cytoskeletons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth I Tang,C Yan ChengPubMed 35971301
- Cell reports 27:2948-2961.e72019Activation of Mevalonate Pathway via LKB1 Is Essential for Stability of T Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaheshwor Timilshina,Zhiwei You,Sonja M Lacher,Suman Acharya,Liyuan Jiang,Youra Kang,Jung-Ae Kim,Hyeun Wook Chang,Keuk-Jun Kim,Byoungduck Park,Jae-Hyoung Song,Hyun-Jeong Ko,Yun-Yong Park,Min-Jung Ma,Mahesh Raj Nepal,Tae Cheon Jeong,Yeonseok Chung,Ari Waisman,Jae-Hoon ChangPubMed 31167140
- Spermatogenesis 2:117-1262012Microtubule affinity-regulating kinase 4 (MARK4) is a component of the ectoplasmic specialization in the rat testis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth I Tang,Xiang Xiao,Dolores D Mruk,Xiao-Jing Qian,Ka-Wai Mok,Pranitha Jenardhanan,Will M Lee,Premendu P Mathur,C Yan ChengPubMed 22670221
- PloS one 7:e315832012LKB1 destabilizes microtubules in myoblasts and contributes to myoblast differentiation.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MARK1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P0L2
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Rat | Predicted | Predicted | Predicted |
| African green monkey | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase (PubMed:23666762). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762). Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).
Additional Targets
MARK2, MARK3, MARK4
Alternative names
KIAA1477, MARK, MARK1, Serine/threonine-protein kinase MARK1, MAP/microtubule affinity-regulating kinase 1, PAR1 homolog c, Par-1c, Par1c
Recommended products
Rabbit Polyclonal MARK1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with African green monkey, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MARK1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MARK1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P0L2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-MARK 1+2+3+4 antibody (ab74131)
All lanes: Western blot - Anti-MARK 1+2+3+4 antibody (ab74131) at 1/500 dilution
Lane 1: Extracts from COS-7 cells at 5 µg
Lane 2: Extracts from COS-7 cells at 5 µg with immunising peptide
Predicted band size: 89 kDa
Observed band size: 89 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARK 1+2+3+4 antibody (ab74131)
Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using ab74131, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Immunocytochemistry/ Immunofluorescence - Anti-MARK 1+2+3+4 antibody (ab74131)
Immunofluorescence analysis of HeLa cells, using ab74131 at 1/100 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
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