AB133724

Anti-MARK2 antibody [EPR8553]

Name: Anti-MARK2 antibody [EPR8553] (ab133724) | Abcam
Brand: Abcam Limited
SKU: AB133724
Price: 695 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(1 Review)

(8 Publications )

Rabbit Recombinant Monoclonal MARK2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 8 publications.

4 Images

Flow Cytometry (Intracellular) - Anti-MARK2 antibody [EPR8553] (AB133724)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-MARK2 antibody [EPR8553] (AB133724)

Intracellular Flow Cytometry analysis of permeabilized Raji cells staining MARK2, using ab133724 at a 1/100 dilution (red) or a control rabbit IgG antibody (green).

Lab

Western blot - Anti-MARK2 antibody [EPR8553] (AB133724)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : MARK2 knockout HAP1 cell lysate (20 μg)
Lane 3 : K562 cell lysate (20 μg)
Lane 4 : Raw264.7 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab133724 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133724 was shown to recognize MARK2 when MARK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MARK2 knockout samples were subjected to SDS-PAGE. ab133724 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)

Predicted band size: 88 kDa

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Western blot - Anti-MARK2 antibody [EPR8553] (AB133724)

All lanes:

Western blot - Anti-MARK2 antibody [EPR8553] (ab133724) at 1/1000 dilution

Lane 1:

RAW 264.7 cell lysate at 10 µg

Lane 2:

NIH/3T3 cell lysate at 10 µg

Lane 3:

Neuro-2a cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Lane 5:

Raji cell lysate at 10 µg

Lane 6:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 88 kDa

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OI-RD Scanning - Anti-MARK2 antibody [EPR8553] (AB133724)

OI-RD Scanning

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OI-RD Scanning - Anti-MARK2 antibody [EPR8553] (AB133724)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-MARK2 antibody [EPR8553] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8553

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase (PubMed : 23666762). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4 and RAB11FIP2. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed : 23666762). Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.

See full target information MARK2

Alternative Names

EMK1, MARK2, Serine/threonine-protein kinase MARK2, ELKL motif kinase 1, MAP/microtubule affinity-regulating kinase 2, PAR1 homolog, PAR1 homolog b, EMK-1, Par-1b, Par1b

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Publications (8)

Recent publications for all applications. Explore the full listand refine your search

eLife 14: PubMed40333320

2025

MARK2 regulates Golgi apparatus reorientation by phosphorylation of CAMSAP2 in directional cell migratio.

Applications

Unspecified application

Species

Unspecified reactive species

Peipei Xu,Rui Zhang,Zhengrong Zhou,Honglin Xu,Yuejia Li,Mengge Yang,Ruifan Lin,Yingchun Wang,Xiahe Huang,Qi Xie,Wenxiang Meng

Journal of inflammation research 17:7261-7274 PubMed39429850

2024

Activating Transcription Factor 6 Mediates Inflammation in Experimental Varicocele-Induced Epididymal Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yin-Shan Jin,Yuan-Qing Cui,Yan-Ping Xu,Jie Chen,Xue-Bao Zhang,Xiong Wang

Cancer discovery 14:2471-2488 PubMed39058094

2024

MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Olaf Klingbeil,Damianos Skopelitis,Claudia Tonelli,Toyoki Yoshimoto,Aktan Alpsoy,Maria C Panepinto,Francesca Minicozzi,Joseph R Merrill,Amanda M Cafiero,Disha Aggarwal,Suzanne Russo,Taehoon Ha,Osama E Demerdash,Tse-Luen Wee,David L Spector,Scott K Lyons,David A Tuveson,Paolo Cifani,Christopher R Vakoc

STAR protocols 2:100430 PubMed33870231

2021

Protocol for visualizing conditional interaction between transmembrane and cytoplasmic proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Ooki,Masanori Hatakeyama

Cancer research 81:2470-2480 PubMed33685991

2021

Genomic Alterations in -Mutated Breast Cancer Result in mTORC1 Activation and Limit the Sensitivity to PI3Kα Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyan Cai,Guotai Xu,Fan Wu,Flavia Michelini,Carmen Chan,Xuan Qu,Pier Selenica,Erik Ladewig,Pau Castel,Yuanming Cheng,Alison Zhao,Komal Jhaveri,Eneda Toska,Marta Jimenez,Alexandra Jacquet,Alicia Tran-Dien,Fabrice Andre,Sarat Chandarlapaty,Jorge S Reis-Filho,Pedram Razavi,Maurizio Scaltriti

American journal of translational research 12:1807-1823 PubMed32509178

2020

MARK2 enhances cisplatin resistance via PI3K/AKT/NF-κB signaling pathway in osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xianfu Wei,Liang Xu,Salim Fa Jeddo,Ka Li,Xin Li,Jianmin Li

Journal of bone oncology 23:100290 PubMed32368441

2020

The inhibition of MARK2 suppresses cisplatin resistance of osteosarcoma stem cells by regulating DNA damage and repair.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Xu,Zhengkao Sun,Xianfu Wei,Hongdong Tan,Peng Kong,Zhenfeng Li,Qiang Yang,E'nuo Dai,Jianmin Li

Developmental cell 49:590-604.e9 PubMed31080060

2019

High-Molecular-Weight Hyaluronan Is a Hippo Pathway Ligand Directing Cell Density-Dependent Growth Inhibition via PAR1b.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Ooki,Naoko Murata-Kamiya,Atsushi Takahashi-Kanemitsu,Weida Wu,Masanori Hatakeyama

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MARK2 antibody [EPR8553]

Flow Cytometry (Intracellular) - Anti-MARK2 antibody [EPR8553] (AB133724)

Western blot - Anti-MARK2 antibody [EPR8553] (AB133724)

Western blot - Anti-MARK2 antibody [EPR8553] (AB133724)

OI-RD Scanning - Anti-MARK2 antibody [EPR8553] (AB133724)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Alternative Names

Publications (8)

Product promise