Anti-MARK2 antibody [EPR8553] - BSA and Azide free

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (AB248629)

This data was developed using ab133724, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of permeabilized Raji cells staining MARK2, using ab133724 at a 1/100 dilution (red) or a control rabbit IgG antibody (green).

• WB

Lab

Western blot - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (AB248629)

This data was developed using ab133724, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 μg)

Lane 2 : MARK2 knockout HAP1 cell lysate (20 μg)

Lane 3 : K562 cell lysate (20 μg)

Lane 4 : Raw264.7 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab133724 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab133724 was shown to recognize MARK2 when MARK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MARK2 knockout samples were subjected to SDS-PAGE. ab133724 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MARK2 antibody [EPR8553] (<a href='/en-us/products/primary-antibodies/mark2-antibody-epr8553-ab133724'>ab133724</a>)

Predicted band size: 88 kDa

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• WB

Unknown

Western blot - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (AB248629)

This data was developed using ab133724, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MARK2 antibody [EPR8553] (<a href='/en-us/products/primary-antibodies/mark2-antibody-epr8553-ab133724'>ab133724</a>) at 1/1000 dilution

Lane 1:

RAW 264.7 cell lysate at 10 µg

Lane 2:

NIH/3T3 cell lysate at 10 µg

Lane 3:

Neuro-2a cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Lane 5:

Raji cell lysate at 10 µg

Lane 6:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 88 kDa

true

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (AB248629)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.