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Rabbit Recombinant Monoclonal MARK2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.

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Images

Western blot - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (AB248629), expandable thumbnail
  • Western blot - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (AB248629), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (AB248629), expandable thumbnail
  • OI-RD Scanning - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (AB248629), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Tested
Not recommended
Expected
Rat
Not recommended
Not recommended
Predicted
Not recommended
Predicted

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human, Mouse

Dilution info

-

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Target data

Function

Serine/threonine-protein kinase (PubMed:23666762). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4 and RAB11FIP2. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762). Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal MARK2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR8553

Purification technique

Affinity purification

Dissociation constant

1.7 x 10-12 M

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab248629 is the carrier-free version of Anti-MARK2 antibody [EPR8553] ab133724.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629), expandable thumbnail

    Western blot - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)

    This data was developed using Anti-MARK2 antibody [EPR8553] ab133724, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 μg)

    Lane 2: MARK2 knockout HAP1 cell lysate (20 μg)

    Lane 3: K562 cell lysate (20 μg)

    Lane 4: Raw264.7 cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-MARK2 antibody [EPR8553] ab133724 observed at 75 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    Anti-MARK2 antibody [EPR8553] ab133724 was shown to recognize MARK2 when MARK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MARK2 knockout samples were subjected to SDS-PAGE. Anti-MARK2 antibody [EPR8553] ab133724 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-MARK2 antibody [EPR8553] (Anti-MARK2 antibody [EPR8553] ab133724)

    Predicted band size: 88 kDa

  • Western blot - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629), expandable thumbnail

    Western blot - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)

    This data was developed using Anti-MARK2 antibody [EPR8553] ab133724, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-MARK2 antibody [EPR8553] (Anti-MARK2 antibody [EPR8553] ab133724) at 1/1000 dilution

    Lane 1: RAW 264.7 cell lysate at 10 µg

    Lane 2: NIH/3T3 cell lysate at 10 µg

    Lane 3: Neuro-2a cell lysate at 10 µg

    Lane 4: Jurkat cell lysate at 10 µg

    Lane 5: Raji cell lysate at 10 µg

    Lane 6: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

    Secondary

    All lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 88 kDa

  • Flow Cytometry (Intracellular) - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)

    This data was developed using Anti-MARK2 antibody [EPR8553] ab133724, the same antibody clone in a different buffer formulation.

    Intracellular Flow Cytometry analysis of permeabilized Raji cells staining MARK2, using Anti-MARK2 antibody [EPR8553] ab133724 at a 1/100 dilution (red) or a control rabbit IgG antibody (green).

  • OI-RD Scanning - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629), expandable thumbnail

    OI-RD Scanning - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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