AB34751

Anti-MARK2 (phospho T595) antibody

Name: Anti-MARK2 (phospho T595) antibody (ab34751) | Abcam
Brand: Abcam Limited
SKU: AB34751

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(7 Publications)

Rabbit Polyclonal MARK2 phospho T595 antibody. Suitable for WB and reacts with Transfected cell lysate - Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MARK2 phospho T595 aa 550-650.

View Alternative Names

EMK1, MARK2, Serine/threonine-protein kinase MARK2, ELKL motif kinase 1, MAP/microtubule affinity-regulating kinase 2, PAR1 homolog, PAR1 homolog b, EMK-1, Par-1b, Par1b

1 Images

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Western blot - Anti-MARK2 (phospho T595) antibody (AB34751)

All lanes:

Western blot - Anti-MARK2 (phospho T595) antibody (ab34751) at 1/500 dilution

Lane 1:

MW markers

Lane 2:

untransfected HEK293 cell lysate

Lane 3:

HEK293 cells transfected with pCMV-3×Flag- MARK2 plus pCMV-Flag-PKC-zeta

Lane 4:

HEK293 cells transfected with pCMV-3×Flag-MARK2 only

Predicted band size: 88 kDa

Observed band size: 82 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

applications

Immunogen

Synthetic Peptide within Human MARK2 phospho T595 aa 550-650. The exact immunogen used to generate this antibody is proprietary information.

Q7KZI7

Specificity

MARK2 pT595 affinity purified antibody is directed against the phosphorylated form of human MARK2 at the pT595 residue. Reactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4. A BLAST analysis was used to suggest reactivity with this protein from human, dog, bovine, mouse and rat sources based on 100% homology for the immunogen sequence. Cross reactivity with MARK2 homologues from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase (PubMed : 23666762). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4 and RAB11FIP2. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed : 23666762). Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.

See full target information MARK2 phospho T595

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 43:5057-5084 PubMed39284914

2024

ER-phagy restrains inflammatory responses through its receptor UBAC2.

Applications

Unspecified application

Species

Unspecified reactive species

Xing He,Haowei He,Zitong Hou,Zheyu Wang,Qinglin Shi,Tao Zhou,Yaoxing Wu,Yunfei Qin,Jun Wang,Zhe Cai,Jun Cui,Shouheng Jin

British journal of pharmacology 181:2012-2032 PubMed38112022

2023

A novel role for microtubule affinity-regulating kinases in neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Qin Shi,Zhi-Hong Sun,Zhe-Zhe Wang,Chun-Yu Su,Wei Zhang,Lin-Yao Yu,Yang Xu,Yong-Lin Gao,Hong-Bo Wang,Jing-Wei Tian,Chun-Mei Li

PLoS biology 19:e3001096 PubMed33705388

2021

MARK2 phosphorylates eIF2α in response to proteotoxic stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ning Lu,Sarah Kavianpour,Tao Zhang,Xumei Zhang,Dao Nguyen,Ravi Thombre,Lu He,Jiou Wang

JCI insight 2: PubMed29093271

2017

Combined inhibition of atypical PKC and histone deacetylase 1 is cooperative in basal cell carcinoma treatment.

Applications

Unspecified application

Species

Mouse

Amar N Mirza,Micah A Fry,Nicole M Urman,Scott X Atwood,Jon Roffey,Gregory R Ott,Bin Chen,Alex Lee,Alexander S Brown,Sumaira Z Aasi,Tyler Hollmig,Mark A Ator,Bruce D Dorsey,Bruce R Ruggeri,Craig A Zificsak,Marina Sirota,Jean Y Tang,Atul Butte,Ervin Epstein,Kavita Y Sarin,Anthony E Oro

Scientific reports 6:34100 PubMed27682244

2016

Regulation of aPKC activity by Nup358 dependent SUMO modification.

Applications

Species

Human

Santosh Kumar Yadav,Indrasen Magre,Aditi Singh,Deepak Khuperkar,Jomon Joseph

The Journal of biological chemistry 291:13809-22 PubMed27143478

2016

Protein Scaffolds Control Localized Protein Kinase Cζ Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Irene S Tobias,Alexandra C Newton

The Journal of biological chemistry 287:12879-85 PubMed22378786

2012

Cellular pharmacology of protein kinase Mζ (PKMζ) contrasts with its in vitro profile: implications for PKMζ as a mediator of memory.

Applications

Species

Rat

Alyssa X Wu-Zhang,Cicely L Schramm,Sadegh Nabavi,Roberto Malinow,Alexandra C Newton

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MARK2 (phospho T595) antibody

Western blot - Anti-MARK2 (phospho T595) antibody (AB34751)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

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