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AB264285

Anti-Mark3 antibody

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(1 Publication)

Rabbit Polyclonal MARK3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MARK3 aa 500-600.

View Alternative Names

CTAK1, EMK2, MARK3, MAP/microtubule affinity-regulating kinase 3, C-TAK1, Cdc25C-associated protein kinase 1, ELKL motif kinase 2, Protein kinase STK10, Ser/Thr protein kinase PAR-1, Serine/threonine-protein kinase p78, cTAK1, EMK-2, Par-1a

2 Images
Immunoprecipitation - Anti-Mark3 antibody (AB264285)
  • IP

Supplier Data

Immunoprecipitation - Anti-Mark3 antibody (AB264285)

Mark3 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264285 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab264285 at 1 μg/ml.

Lane 1 : ab264285 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 10 secs.

All lanes:

Immunoprecipitation - Anti-Mark3 antibody (ab264285)

Predicted band size: 87 kDa

false

Western blot - Anti-Mark3 antibody (AB264285)
  • WB

Supplier Data

Western blot - Anti-Mark3 antibody (AB264285)

All lanes:

Western blot - Anti-Mark3 antibody (ab264285) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 87 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human MARK3 aa 500-600. The exact immunogen used to generate this antibody is proprietary information.

P27448

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase (PubMed : 16822840, PubMed : 16980613, PubMed : 23666762). Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed : 23666762). Phosphorylates CDC25C on 'Ser-216' (PubMed : 12941695). Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus (PubMed : 16980613). Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol (PubMed : 16822840). Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1 (PubMed : 28087714). Phosphorylates PKP2 and KSR1 (PubMed : 12941695).
See full target information MARK3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer discovery 14:2471-2488 PubMed39058094

2024

MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Olaf Klingbeil,Damianos Skopelitis,Claudia Tonelli,Toyoki Yoshimoto,Aktan Alpsoy,Maria C Panepinto,Francesca Minicozzi,Joseph R Merrill,Amanda M Cafiero,Disha Aggarwal,Suzanne Russo,Taehoon Ha,Osama E Demerdash,Tse-Luen Wee,David L Spector,Scott K Lyons,David A Tuveson,Paolo Cifani,Christopher R Vakoc
View all publications
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Product promise

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