Rabbit Recombinant Monoclonal MARK4 phospho T214 + MARK2 phospho T208 + MARK3 phospho T234 + MARK1 phospho T215 antibody. Suitable for Dot, WB, I-ELISA and reacts with Human amd Synthetic peptide samples.
Images
![Western blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (AB126731), expandable thumbnail](https://content.abcam.com/products/images/mark4-phospho-t214-mark2-phospho-t208-mark3-phospho-t234-mark1-phospho-t215-antibody-epr5463-ab126731--western-blot-img178539.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (AB126731)")
![Indirect ELISA - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (AB126731), expandable thumbnail](https://content.abcam.com/products/images/mark4-phospho-t214-mark2-phospho-t208-mark3-phospho-t234-mark1-phospho-t215-antibody-epr5463-ab126731--indirect-elisa-img170894.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (AB126731)")
![Dot Blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (AB126731), expandable thumbnail](https://content.abcam.com/products/images/mark4-phospho-t214-mark2-phospho-t208-mark3-phospho-t234-mark1-phospho-t215-antibody-epr5463-ab126731--dot-blot-img167779.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (AB126731)")
![Western blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (AB126731), expandable thumbnail](https://content.abcam.com/products/images/mark4-phospho-t214-mark2-phospho-t208-mark3-phospho-t234-mark1-phospho-t215-antibody-epr5463-ab126731--western-blot-img109123.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (AB126731)")
![OI-RD Scanning - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (AB126731), expandable thumbnail](https://content.abcam.com/products/images/mark4-phospho-t214-mark2-phospho-t208-mark3-phospho-t234-mark1-phospho-t215-antibody-epr5463-ab126731--oi-rd-scanning-img98400.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (AB126731)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC-P | Flow Cyt | ICC/IF | Dot | WB | I-ELISA | |
|---|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Expected | Tested | Expected |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Predicted | Predicted | Predicted |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Predicted | Predicted | Predicted |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Synthetic peptide - Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide - Human, Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide - Human, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide - Human, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide - Human, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Synthetic peptide | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Serine/threonine-protein kinase (PubMed:23666762). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4 and RAB11FIP2. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762). Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.
Additional Targets
MARK3 phospho T234, MARK4 phospho T214, MARK1 phospho T215
Alternative names
EMK1, MARK2, Serine/threonine-protein kinase MARK2, ELKL motif kinase 1, MAP/microtubule affinity-regulating kinase 2, PAR1 homolog, PAR1 homolog b, EMK-1, Par-1b, Par1b
Recommended products
Rabbit Recombinant Monoclonal MARK4 phospho T214 + MARK2 phospho T208 + MARK3 phospho T234 + MARK1 phospho T215 antibody. Suitable for Dot, WB, I-ELISA and reacts with Human amd Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5463
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody detects MARK4 phosphorylated at Threonine 214, MARK2 phosphorylated at Threonine 208, MARK3 phosphorylated at Threonine 234 and MARK1 phosphorylated at Threonine 215.
- Dissociation constant
- 1.62 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731), expandable thumbnail](https://content.abcam.com/products/images/mark4-phospho-t214-mark2-phospho-t208-mark3-phospho-t234-mark1-phospho-t215-antibody-epr5463-ab126731--western-blot-img178539.jpg "Western blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731)")
Western blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731)
Westernblot analysis of ab126731 at a 1/20 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as a scondary antibody at 1/20000 dilution.
Lane 1: HEK-293 (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate 15μg
Lane 2: HEK-293 transfected with MARK1 expression vector containing a myc-His-tag®, whole cell lysate 15μg
Lane 3: HEK-293 transfected with MARK1 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 4: HEK-293 transfected with MARK2 expression vector containing a myc-His-tag®, whole cell lysate 15μg
Lane 5: HEK-293 transfected with MARK2 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 6: HEK-293 transfected with MARK3 expression vector containing a myc-His-tag®, whole cell lysate 15μg
Lane 7: HEK-293 transfected with MARK3 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 8: HEK-293 transfected with MARK4 expression vector containing a myc-His-tag®, whole cell lysate 15μg
Lane 9: HEK-293 transfected with MARK4 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour
Exposure time:
Lane 1 – 3, 6 - 9: 180 seconds
Lane 4 - 5: 7 seconds
Endogenous MARK proteins are observed at ~110 kDa reported by PMID: 9108484 and 16238695
All lanes: Western blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731)
![Indirect ELISA - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731), expandable thumbnail](https://content.abcam.com/products/images/mark4-phospho-t214-mark2-phospho-t208-mark3-phospho-t234-mark1-phospho-t215-antibody-epr5463-ab126731--indirect-elisa-img170894.jpg "Indirect ELISA - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731)")
Indirect ELISA - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731)
Indirect ELISA antibody dose-response curve using ab126731 between 0-10000 ng/ml. Antigen concentration of 500 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
![Dot Blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731), expandable thumbnail](https://content.abcam.com/products/images/mark4-phospho-t214-mark2-phospho-t208-mark3-phospho-t234-mark1-phospho-t215-antibody-epr5463-ab126731--dot-blot-img167779.jpg "Dot Blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731)")
Dot Blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731)
Dot Blot using ab126731 at a 1/1000 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at a 1/100000 dilution
Lane 1: Human MARK1 (T215) phospho peptide
Lane 2: Human MARK1 non-phospho peptide
Lane 3: Human MARK2 (T208) phospho peptide
Lane 4: Human MARK2 non-phospho peptide
Lane 5: Human MARK3 (T234) phospho peptide
Lane 6: Human MARK3 non-phospho peptide
Lane 7: Human MARK4 (T214) phospho peptide
Lane 8: Human MARK4 non-phospho peptide
![Western blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731), expandable thumbnail](https://content.abcam.com/products/images/mark4-phospho-t214-mark2-phospho-t208-mark3-phospho-t234-mark1-phospho-t215-antibody-epr5463-ab126731--western-blot-img109123.jpg "Western blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731)")
Western blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731)
All lanes: Western blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731) at 1/10000 dilution
Lane 1: SH-SY5Y cell lysate, untreated at 10 µg
Lane 2: SH-SY5Y cell lysate, treated with Okadaic acid and Calyculin A at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
![OI-RD Scanning - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731), expandable thumbnail](https://content.abcam.com/products/images/mark4-phospho-t214-mark2-phospho-t208-mark3-phospho-t234-mark1-phospho-t215-antibody-epr5463-ab126731--oi-rd-scanning-img98400.jpg "OI-RD Scanning - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731)")
OI-RD Scanning - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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