AB126731

Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal MARK4 phospho T214 + MARK2 phospho T208 + MARK3 phospho T234 + MARK1 phospho T215 antibody. Suitable for Dot, WB, I-ELISA and reacts with Human amd Synthetic peptide samples.

View Alternative Names

EMK1, MARK2, Serine/threonine-protein kinase MARK2, ELKL motif kinase 1, MAP/microtubule affinity-regulating kinase 2, PAR1 homolog, PAR1 homolog b, EMK-1, Par-1b, Par1b, KIAA1477, MARK, MARK1, Serine/threonine-protein kinase MARK1, MAP/microtubule affinity-regulating kinase 1, PAR1 homolog c, Par-1c, Par1c, CTAK1, EMK2, MARK3, MAP/microtubule affinity-regulating kinase 3, C-TAK1, Cdc25C-associated protein kinase 1, ELKL motif kinase 2, Protein kinase STK10, Ser/Thr protein kinase PAR-1, Serine/threonine-protein kinase p78, cTAK1, EMK-2, Par-1a, KIAA1860, MARKL1, MARK4, MAP/microtubule affinity-regulating kinase 4, MAP/microtubule affinity-regulating kinase-like 1

5 Images

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Western blot - Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463] (AB126731)

All lanes:

Western blot - Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463] (ab126731) at 1/10000 dilution

Lane 1:

SH-SY5Y cell lysate, untreated at 10 µg

Lane 2:

SH-SY5Y cell lysate, treated with Okadaic acid and Calyculin A at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

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Western blot - Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463] (AB126731)

Westernblot analysis of ab126731 at a 1/20 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as a scondary antibody at 1/20000 dilution.

Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate 15μg

Lane 2 : HEK-293 transfected with MARK1 expression vector containing a myc-His-tag®, whole cell lysate 15μg

Lane 3 : HEK-293 transfected with MARK1 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour

Lane 4 : HEK-293 transfected with MARK2 expression vector containing a myc-His-tag®, whole cell lysate 15μg

Lane 5 : HEK-293 transfected with MARK2 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour

Lane 6 : HEK-293 transfected with MARK3 expression vector containing a myc-His-tag®, whole cell lysate 15μg

Lane 7 : HEK-293 transfected with MARK3 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour

Lane 8 : HEK-293 transfected with MARK4 expression vector containing a myc-His-tag®, whole cell lysate 15μg

Lane 9 : HEK-293 transfected with MARK4 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour

Exposure time :

Lane 1 – 3, 6 - 9 : 180 seconds

Lane 4 - 5 : 7 seconds

Endogenous MARK proteins are observed at ~110 kDa reported by PMID : 9108484 and 16238695

All lanes:

Western blot - Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463] (ab126731)

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Unknown

Dot Blot - Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463] (AB126731)

Dot Blot using ab126731 at a 1/1000 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100000 dilution

Lane 1 : Human MARK1 (T215) phospho peptide

Lane 2 : Human MARK1 non-phospho peptide

Lane 3 : Human MARK2 (T208) phospho peptide

Lane 4 : Human MARK2 non-phospho peptide

Lane 5 : Human MARK3 (T234) phospho peptide

Lane 6 : Human MARK3 non-phospho peptide

Lane 7 : Human MARK4 (T214) phospho peptide

Lane 8 : Human MARK4 non-phospho peptide

Indirect ELISA - Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463] (AB126731)

I-ELISA

Unknown

Indirect ELISA - Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463] (AB126731)

Indirect ELISA antibody dose-response curve using ab126731 between 0-10000 ng/ml. Antigen concentration of 500 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

OI-RD Scanning - Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463] (AB126731)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463] (AB126731)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-MARK4(pT214) + MARK2(pT208) + MARK3(pT234) + MARK1(pT215) antibody [EPR5463] - BSA & Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5463

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Dot, WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects MARK4 phosphorylated at Threonine 214, MARK2 phosphorylated at Threonine 208, MARK3 phosphorylated at Threonine 234 and MARK1 phosphorylated at Threonine 215.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase (PubMed : 23666762). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4 and RAB11FIP2. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed : 23666762). Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.

See full target information MARK2 phospho T208

Additional targets

MARK3 phospho T234,MARK4 phospho T214,MARK1 phospho T215

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Genes 16: PubMed40869977

2025

The Transcriptional Coactivator Gene Is a Target of the Transcription Factor E2F1 Deregulated from the Tumor Suppressor pRB.

Applications

Unspecified application

Species

Unspecified reactive species

Rinka Nakajima,Yaxuan Zhou,Mashiro Shirasawa,Mariana Fikriyanti,Ritsuko Iwanaga,Andrew P Bradford,Kenta Kurayoshi,Keigo Araki,Kiyoshi Ohtani

British journal of pharmacology 181:2012-2032 PubMed38112022

2023

A novel role for microtubule affinity-regulating kinases in neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Qin Shi,Zhi-Hong Sun,Zhe-Zhe Wang,Chun-Yu Su,Wei Zhang,Lin-Yao Yu,Yang Xu,Yong-Lin Gao,Hong-Bo Wang,Jing-Wei Tian,Chun-Mei Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463]

Western blot - Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463] (AB126731)

Western blot - Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463] (AB126731)

Dot Blot - Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463] (AB126731)

Indirect ELISA - Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463] (AB126731)

OI-RD Scanning - Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463] (AB126731)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (2)

Product promise