AB248152

Anti-MARK4(pT214) + MARK2(pT208) + MARK3(pT234) + MARK1(pT215) antibody [EPR5463] - BSA & Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MARK4 phospho T214 + MARK2 phospho T208 + MARK3 phospho T234 + MARK1 phospho T215 antibody. Carrier free. Suitable for I-ELISA, Dot, WB and reacts with Human and Synthetic peptide samples.

View Alternative Names

KIAA1477, MARK, MARK1, Serine/threonine-protein kinase MARK1, MAP/microtubule affinity-regulating kinase 1, PAR1 homolog c, Par-1c, Par1c, EMK1, MARK2, Serine/threonine-protein kinase MARK2, ELKL motif kinase 1, MAP/microtubule affinity-regulating kinase 2, PAR1 homolog, PAR1 homolog b, EMK-1, Par-1b, Par1b, CTAK1, EMK2, MARK3, MAP/microtubule affinity-regulating kinase 3, C-TAK1, Cdc25C-associated protein kinase 1, ELKL motif kinase 2, Protein kinase STK10, Ser/Thr protein kinase PAR-1, Serine/threonine-protein kinase p78, cTAK1, EMK-2, Par-1a, KIAA1860, MARKL1, MARK4, MAP/microtubule affinity-regulating kinase 4, MAP/microtubule affinity-regulating kinase-like 1

5 Images

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Western blot - Anti-MARK4(pT214) + MARK2(pT208) + MARK3(pT234) + MARK1(pT215) antibody [EPR5463] - BSA & Azide free (AB248152)

This data was developed using ab126731, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463] (<a href='/en-us/products/primary-antibodies/mark4-phospho-t214-mark2-phospho-t208-mark3-phospho-t234-mark1-phospho-t215-antibody-epr5463-ab126731'>ab126731</a>) at 1/10000 dilution

Lane 1:

SH-SY5Y cell lysate, untreated at 10 µg

Lane 2:

SH-SY5Y cell lysate, treated with Okadaic acid and Calyculin A at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

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Western blot - Anti-MARK4(pT214) + MARK2(pT208) + MARK3(pT234) + MARK1(pT215) antibody [EPR5463] - BSA & Azide free (AB248152)

Westernblot analysis of ab126731 at a 1/20 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as a scondary antibody at 1/20000 dilution.

Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate 15μg

Lane 2 : HEK-293 transfected with MARK1 expression vector containing a myc-His-tag®, whole cell lysate 15μg

Lane 3 : HEK-293 transfected with MARK1 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour

Lane 4 : HEK-293 transfected with MARK2 expression vector containing a myc-His-tag®, whole cell lysate 15μg

Lane 5 : HEK-293 transfected with MARK2 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour

Lane 6 : HEK-293 transfected with MARK3 expression vector containing a myc-His-tag®, whole cell lysate 15μg

Lane 7 : HEK-293 transfected with MARK3 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour

Lane 8 : HEK-293 transfected with MARK4 expression vector containing a myc-His-tag®, whole cell lysate 15μg

Lane 9 : HEK-293 transfected with MARK4 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour

Exposure time :

Lane 1 – 3, 6 - 9 : 180 seconds

Lane 4 - 5 : 7 seconds

Endogenous MARK proteins are observed at ~110 kDa reported by PMID : 9108484 and 16238695

All lanes:

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Dot Blot - Anti-MARK4(pT214) + MARK2(pT208) + MARK3(pT234) + MARK1(pT215) antibody [EPR5463] - BSA & Azide free (AB248152)

Dot Blot using ab126731 at a 1/1000 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100000 dilution

Lane 1 : Human MARK1 (T215) phospho peptide

Lane 2 : Human MARK1 non-phospho peptide

Lane 3 : Human MARK2 (T208) phospho peptide

Lane 4 : Human MARK2 non-phospho peptide

Lane 5 : Human MARK3 (T234) phosphor peptide

Lane 6 : Human MARK3 non-phospho peptide

Lane 7 : Human MARK4 (T214) phospho peptide

Lane 8 : Human MARK4 non-phospho peptide

Indirect ELISA - Anti-MARK4(pT214) + MARK2(pT208) + MARK3(pT234) + MARK1(pT215) antibody [EPR5463] - BSA & Azide free (AB248152)

I-ELISA

Unknown

Indirect ELISA - Anti-MARK4(pT214) + MARK2(pT208) + MARK3(pT234) + MARK1(pT215) antibody [EPR5463] - BSA & Azide free (AB248152)

Indirect ELISA antibody dose-response curve using ab126731 between 0-10000 ng/ml. Antigen concentration of 500 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

OI-RD Scanning - Anti-MARK4(pT214) + MARK2(pT208) + MARK3(pT234) + MARK1(pT215) antibody [EPR5463] - BSA & Azide free (AB248152)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-MARK4(pT214) + MARK2(pT208) + MARK3(pT234) + MARK1(pT215) antibody [EPR5463] - BSA & Azide free (AB248152)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-MARK4 (pT214) + MARK2 (pT208) + MARK3 (pT234) + MARK1 (pT215) antibody [EPR5463]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5463

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

I-ELISA, WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects MARK4 phosphorylated at Threonine 214, MARK2 phosphorylated at Threonine 208, MARK3 phosphorylated at Threonine 234 and MARK1 phosphorylated at Threonine 215.

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Reactivity data

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Product details

ab248152 is the carrier-free version of ab126731.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase (PubMed : 23666762). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed : 23666762). Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).

See full target information MARK1 pT215

Additional targets

MARK4 phospho T214,MARK2 phospho T208,MARK3 phospho T234

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MARK4(pT214) + MARK2(pT208) + MARK3(pT234) + MARK1(pT215) antibody [EPR5463] - BSA & Azide free

Western blot - Anti-MARK4(pT214) + MARK2(pT208) + MARK3(pT234) + MARK1(pT215) antibody [EPR5463] - BSA & Azide free (AB248152)

Western blot - Anti-MARK4(pT214) + MARK2(pT208) + MARK3(pT234) + MARK1(pT215) antibody [EPR5463] - BSA & Azide free (AB248152)

Dot Blot - Anti-MARK4(pT214) + MARK2(pT208) + MARK3(pT234) + MARK1(pT215) antibody [EPR5463] - BSA & Azide free (AB248152)

Indirect ELISA - Anti-MARK4(pT214) + MARK2(pT208) + MARK3(pT234) + MARK1(pT215) antibody [EPR5463] - BSA & Azide free (AB248152)

OI-RD Scanning - Anti-MARK4(pT214) + MARK2(pT208) + MARK3(pT234) + MARK1(pT215) antibody [EPR5463] - BSA & Azide free (AB248152)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Product promise