Anti-MARVELD2/Tricellulin antibody [54H19L38]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MARVELD2/Tricellulin antibody [54H19L38] (AB203567)

Immunofluorescence analysis of Tricellulin was done on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ab203567 at 1 μg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300. Panel d is a merged image showing cell junctional localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MARVELD2/Tricellulin antibody [54H19L38] (AB203567)

Immunofluorescent analysis of Caco 2 cells labeling MARVELD2/Tricellulin using ab203567 at 8 μg/mL. Alexa Fluor® 488 goat anti-rabbit at 1/1000 was used as secondary antibody (green). DAPI staining of nucleus (top left, blue), cell-cell junctions were labeled using a anti- Occludin antibody (top right, red). Bottom right : merged image.

• IP

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Immunoprecipitation - Anti-MARVELD2/Tricellulin antibody [54H19L38] (AB203567)

Tricellulin was immunoprecipitated using 5 μg of ab203567 from lysate of HeLa (Lane 3). Normal Rabbit IgG was used as a Isotype control (Lane 2). 10 % input represents the cell extract used for immunoprecipitation (Lane 1). Western blot analysis was performed using ab203567 and Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1 : 2500 dilution. Chemiluminescent detection was performed using ECL Western Blotting Substrate.

All lanes:

Immunoprecipitation - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)

Predicted band size: 64 kDa

false

• WB

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Western blot - Anti-MARVELD2/Tricellulin antibody [54H19L38] (AB203567)

All lanes:

Western blot - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567) at 1 µg/mL

Lane 1:

HeLa cell lysate at 30 µg

Lane 2:

PANC1 cell lysate at 30 µg

Lane 3:

MCF-7 cell lysate at 30 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2500 dilution

Predicted band size: 64 kDa

false

• WB

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Western blot - Anti-MARVELD2/Tricellulin antibody [54H19L38] (AB203567)

All lanes:

Western blot - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567) at 2 µg

Lane 1:

Untransfected HeLa cells

Lane 2:

HeLa cells transfected with scrambled SiRNA

Lane 3:

HeLa cells transfected with MARVELD2/Tricellulin siRNA

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) secondary antibody at 1/40000 dilution

Predicted band size: 64 kDa

false

• WB

Supplier Data

Western blot - Anti-MARVELD2/Tricellulin antibody [54H19L38] (AB203567)

All lanes:

Western blot - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567) at 3 µg/mL

Lane 1:

Caco 2 cell lysate

Lane 2:

MDCK cell lysate

Predicted band size: 109 kDa,64 kDa

false