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AB249257

Anti-MAS1L antibody [EPR11440(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal MAS1L antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

MRG, MAS1L, Mas-related G-protein coupled receptor MRG, MAS-R, MAS1-like

3 Images
Flow Cytometry (Intracellular) - Anti-MAS1L antibody [EPR11440(B)] - BSA and Azide free (AB249257)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-MAS1L antibody [EPR11440(B)] - BSA and Azide free (AB249257)

This data was developed using ab156018, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293T cells labeling MAS1L with ab156018 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Immunoprecipitation - Anti-MAS1L antibody [EPR11440(B)] - BSA and Azide free (AB249257)
  • IP

Unknown

Immunoprecipitation - Anti-MAS1L antibody [EPR11440(B)] - BSA and Azide free (AB249257)

This data was developed using ab156018, the same antibody clone in a different buffer formulation.Immunoprecipitation analysis of HepG2 cell lysates using ab156018 at 1/100 dilution (Lane 1) or rabbit IgG (Lane 2).

All lanes:

Immunoprecipitation - Anti-MAS1L antibody [EPR11440(B)] (<a href='/en-us/products/primary-antibodies/mas1l-antibody-epr11440b-ab156018'>ab156018</a>)

Predicted band size: 42 kDa

false

Western blot - Anti-MAS1L antibody [EPR11440(B)] - BSA and Azide free (AB249257)
  • WB

Unknown

Western blot - Anti-MAS1L antibody [EPR11440(B)] - BSA and Azide free (AB249257)

This data was developed using ab156018, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MAS1L antibody [EPR11440(B)] (<a href='/en-us/products/primary-antibodies/mas1l-antibody-epr11440b-ab156018'>ab156018</a>) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

Y79 (Human retinoblastoma cell line) cell lysate at 10 µg

Lane 5:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11440(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249257 is the carrier-free version of ab156018.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAS1L sometimes referred to as MAS1-like is a protein encoded by the MAS1L gene. Its mass is approximately 37 kDa. This protein is expressed in various tissues including the central nervous system and certain peripheral tissues. MAS1L is an integral membrane protein and it localizes on the cell surface which indicates its potential involvement in signaling processes. As a G protein-coupled receptor it plays a role in transmitting signals across the cell membrane.
Biological function summary

MAS1L functions within the context of signal transduction. It engages in biological processes by potentially forming complexes with other proteins or molecules that facilitate signaling activities. This protein's ability to interact with G proteins suggests its involvement in activating downstream cellular responses influencing various cellular functions such as growth differentiation or communication between cells. The MAS1L's role in signaling implicates it in maintaining normal cellular activity and responding to environmental cues.

Pathways

MAS1L engages in signaling cascades potentially linked to the renin-angiotensin system and other related pathways that influence cardiovascular functions. In the renin-angiotensin system similar receptors like the MAS1 receptor participate suggesting that MAS1L could share functional similarities. The renin-angiotensin pathway plays an important role in regulating blood pressure and fluid balance and any interaction involving MAS1L might contribute to these physiological processes.

Abnormal expression or mutation of MAS1L could relate to cardiovascular diseases and neurological disorders. Dysfunctional signaling through MAS1L might contribute to the pathology of hypertension a condition in which the MAS1 receptor also plays a significant role highlighting potential overlap in their functional pathways. Furthermore disruptions in MAS1L activity might affect neurological functions linking it to conditions that compromise nervous system integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MAS1L
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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