Anti-MASH1/Achaete-scute homolog 1 antibody

• WB

Unknown

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (AB74065)

All lanes:

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) at 1 µg/mL

Lane 1:

DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Brain (Rat) Tissue Lysate at 10 µg

Lane 3:

Brain (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa,28 kDa,70 kDa

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• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody (AB74065)

ab74065 staining MASH1/Achaete-scute homolog 1 in SK-N-SH cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab74065 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• WB

Unknown

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (AB74065)

All lanes:

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) at 1 µg/mL

Lane 2:

Zebrafish brain homogenate at 20 µg

Lane 3:

Mouse brain homogenate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

Predicted band size: 25 kDa

Observed band size: 26 kDa

true

Exposure time: 5min

• WB

CiteAb

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (AB74065)

MASH1/Achaete-scute homolog 1 western blot using anti-MASH1/Achaete-scute homolog 1 antibody ab74065. Publication image and figure legend from Ghazale, H., Park, E., et al., 2022, Front Neurosci, PubMed 36061596.

ab74065 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab74065 please see the product overview.

Ascl1 is phosphorylated by proline-directed serine-threonine kinases in the adult brain in vivo.(A) Schematic illustration of the sequence of wild-type (wt) Ascl1 with SP sites designated, and Ascl1SA6, with SA mutations indicated. (B) Schematic illustration of AAV8-GFAPlong-mCherry vectors. (C,D) Western blot analysis of lysates from right (sample 1) and left (sample 2) cortical hemispheres transduced with AAV8-GFAPlong-mCherry (mCh), AAV8-GFAPlong-Ascl1-mCherry (Ascl1) or AAV8-GFAPlong-Ascl1SA6-mCherry (Ascl1SA6), analyzed for the expression of pERK, ERK, and β-actin (C), or GSK3, CDK1, and β-actin (D). (E) Ascl1 expression in the ventricular-subventricular zone (V-SVZ) in a coronal section of the adult cortex. (F) Phos-tag western blot of cortical lysates from left (sample 1); left (sample 2); and right (sample 3) cortical hemispheres transduced with mCherry or Ascl1-mCherry in two independent experiments shown in separate gels either treated or not with Lambda protein phosphatase (ph'tase) and blotted with anti-Ascl1 (Abcam). Scale bars in panel E = 100 μm. ct, cortex; lv, lateral ventricle; rms, rostral migratory stream; str, striatum; v-svz, ventricular-subventricular zone. Significance was defined as p-values less than 0.05 and denoted as follows : ns, not significant, <0.05 *, <0.01 **, <0.001 ***.

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