Rabbit Polyclonal MASH1/Achaete-scute homolog 1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Zebrafish, Human samples. Cited in 43 publications.
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- Nature communications 14:63412023Subventricular zone cytogenesis provides trophic support for neural repair in a mouse model of stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael R Williamson,Stephanie P Le,Ronald L Franzen,Nicole A Donlan,Jill L Rosow,Mathilda S Nicot-Cartsonis,Alexis Cervantes,Benjamin Deneen,Andrew K Dunn,Theresa A Jones,Michael R DrewPubMed 37816732
- Neural development 18:52023Distinct requirements for Tcf3 and Tcf12 during oligodendrocyte development in the mouse telencephalon.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMary Jo Talley,Diana Nardini,Lisa A Ehrman,Q Richard Lu,Ronald R WaclawPubMed 37684687
- Frontiers in molecular neuroscience 16:11511622023Glycolysis mediates neuron specific histone acetylation in valproic acid-induced human excitatory neuron differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndi Chen,Mengmeng Wang,Chao Xu,Youyi Zhao,Panpan Xian,Yuqian Li,Weian Zheng,Xuyang Yi,Shengxi Wu,Yazhou WangPubMed 37089691
- Cell stem cell 29:1491-1504.e92022Cross-lineage potential of Ascl1 uncovered by comparing diverse reprogramming regulatomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaofei Wang,Benjamin Keepers,Yunzhe Qian,Yifang Xie,Marazzano Colon,Jiandong Liu,Li QianPubMed 36206732
- Frontiers in neuroscience 16:9170712022Ascl1 phospho-site mutations enhance neuronal conversion of adult cortical astrocytes .Applications:Unspecified applicationReactive species:Unspecified reactive speciesHussein Ghazale,EunJee Park,Lakshmy Vasan,James Mester,Fermisk Saleh,Andrea Trevisiol,Dawn Zinyk,Vorapin Chinchalongporn,Mingzhe Liu,Taylor Fleming,Oleksandr Prokopchuk,Natalia Klenin,Deborah Kurrasch,Maryam Faiz,Bojana Stefanovic,JoAnne McLaurin,Carol SchuurmansPubMed 36061596
- Frontiers in neuroscience 16:9038812022Foxa2 and Pet1 Direct and Indirect Synergy Drive Serotonergic Neuronal Differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBegüm Aydin,Michael Sierk,Mireia Moreno-Estelles,Link Tejavibulya,Nikathan Kumar,Nuria Flames,Shaun Mahony,Esteban O MazzoniPubMed 35801179
- BMC genomics 23:2552022Elevated ASCL1 activity creates de novo regulatory elements associated with neuronal differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura M Woods,Fahad R Ali,Roshna Gomez,Igor Chernukhin,Daniel Marcos,Lydia M Parkinson,Ahmad N Abou Tayoun,Jason S Carroll,Anna PhilpottPubMed 35366798
- Frontiers in immunology 13:8054512022The Lineage Differentiation and Dynamic Heterogeneity of Thymic Epithelial Cells During Thymus Organogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanchao Gao,Mengtao Cao,Kai Deng,Yang Yang,Jinqi Song,Ming Ni,Chuntao Xie,Wenna Fan,Chunpei Ou,Dinggen Huang,Lizhong Lin,Lixia Liu,Yangyang Li,Huimin Sun,Xinyu Cheng,Jinmei Wu,Cuilan Xia,Xuefeng Deng,Lisha Mou,Pengfei ChenPubMed 35273595
- Scientific reports 12:23412022ASCL1 phosphorylation and ID2 upregulation are roadblocks to glioblastoma stem cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoberta Azzarelli,Aoibheann McNally,Claudia Dell'Amico,Marco Onorati,Benjamin Simons,Anna PhilpottPubMed 35149717
- Cancers 14:2022c-Myc Targets HDAC3 to Suppress NKG2DL Expression and Innate Immune Response in N-Type SCLC through Histone Deacetylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeiyan Zhao,Xiaodan Sun,Hui Li,Yan Liu,Yanan Cui,Lin Tian,Ying ChengPubMed 35158730
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Chicken | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Zebrafish | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Zebrafish | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Dog, Xenopus laevis, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Dog, Xenopus laevis, Rhesus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach (By similarity).
Alternative names
ASH1, BHLHA46, HASH1, ASCL1, Achaete-scute homolog 1, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46
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Rabbit Polyclonal MASH1/Achaete-scute homolog 1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Zebrafish, Human samples. Cited in 43 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Ab74065 has been batch tested in WB and IF only. Some customers have successfully used this product in IHC. Please contact Abcam Scientific Support for more information.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MASH1 protein also known as Achaete-Scute homolog 1 (ASCL1) plays an important role in neurogenesis. It acts as a transcription factor and regulates neuronal differentiation. MASH1 has a molecular weight of approximately 34 kDa. It expresses predominantly in the nervous system including the developing neural tube and peripheral ganglia and it can also be found in certain regions of the brain during embryonic development. ASCL1 is part of the basic helix-loop-helix (bHLH) family of transcription factors which are key regulators of neuronal identity and function.
- Biological function summary
This protein regulates the expression of genes important for the development of neuronal precursors. MASH1 does not act alone; it forms heterodimers with other bHLH proteins which enhance its ability to bind DNA and influence gene transcription. It helps convert multipotent neural progenitors into committed neuronal precursors which later differentiate into specific neuronal cell types. MASH1's role is particularly important during early stages of neuronal differentiation ensuring proper formation and maturation of the nervous system.
- Pathways
MASH1 is central to the Notch signaling pathway and other pathways involved in neural differentiation. Within the Notch pathway MASH1 interacts with proteins like HES1 to balance the differentiation of neural progenitor cells into neurons and glial cells. This protein also connects with other pathway components such as NEUROG2 a downstream target to promote neuronal fate specification. These interactions illustrate MASH1’s integration into complex signaling networks that govern cell fate decisions during neurogenesis.
- Associated diseases and disorders
MASH1 has significant associations with neurodevelopmental disorders and certain cancers. Aberrant ASCL1 activity links to conditions like Hirschsprung's disease where neural crest development is impaired and some types of lung cancer particularly pulmonary neuroendocrine tumors. It is known to interact with the RET protein in the context of Hirschsprung's disease and with proteins like MYC in cancer pathways highlighting its multifaceted involvement in pathology. Understanding these associations helps elucidate the mechanisms of these diseases and supports therapeutic target development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065)
All lanes: Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) at 1 µg/mL
Lane 1: DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Brain (Rat) Tissue Lysate at 10 µg
Lane 3: Brain (Mouse) Tissue Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa, 28 kDa, 70 kDa
Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065)
All lanes: Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) at 1 µg/mL
Lane 1: DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Brain (Rat) Tissue Lysate at 10 µg
Lane 3: Brain (Mouse) Tissue Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 3min
Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065)
All lanes: Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) at 1 µg/mL
Lane 2: Zebrafish brain homogenate at 20 µg
Lane 3: Mouse brain homogenate at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 26 kDa
Exposure time: 5min
Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065)
ab74065 staining MASH1/Achaete-scute homolog 1 in SK-N-SH cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab74065 at 5µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065)
MASH1/Achaete-scute homolog 1 western blot using anti-MASH1/Achaete-scute homolog 1 antibody ab74065. Publication image and figure legend from Ghazale, H., Park, E., et al., 2022, Front Neurosci, PubMed 36061596.
ab74065 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab74065 please see the product overview.
Ascl1 is phosphorylated by proline-directed serine-threonine kinases in the adult brain in vivo.(A) Schematic illustration of the sequence of wild-type (wt) Ascl1 with SP sites designated, and Ascl1SA6, with SA mutations indicated. (B) Schematic illustration of AAV8-GFAPlong-mCherry vectors. (C,D) Western blot analysis of lysates from right (sample 1) and left (sample 2) cortical hemispheres transduced with AAV8-GFAPlong-mCherry (mCh), AAV8-GFAPlong-Ascl1-mCherry (Ascl1) or AAV8-GFAPlong-Ascl1SA6-mCherry (Ascl1SA6), analyzed for the expression of pERK, ERK, and β-actin (C), or GSK3, CDK1, and β-actin (D). (E) Ascl1 expression in the ventricular-subventricular zone (V-SVZ) in a coronal section of the adult cortex. (F) Phos-tag western blot of cortical lysates from left (sample 1); left (sample 2); and right (sample 3) cortical hemispheres transduced with mCherry or Ascl1-mCherry in two independent experiments shown in separate gels either treated or not with Lambda protein phosphatase (ph’tase) and blotted with anti-Ascl1 (Abcam). Scale bars in panel E = 100 μm. ct, cortex; lv, lateral ventricle; rms, rostral migratory stream; str, striatum; v-svz, ventricular-subventricular zone. Significance was defined as p-values less than 0.05 and denoted as follows: ns, not significant, <0.05 *, <0.01 **, <0.001 ***.
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