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AB213151

Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592]

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(6 Publications)

Rabbit Recombinant Monoclonal MASH1/Achaete-scute homolog 1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 6 publications.

View Alternative Names

ASH1, BHLHA46, HASH1, ASCL1, Achaete-scute homolog 1, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)

Immunohistochemical analysis of paraffin-embedded human medullary thyroid carcinoma tissue labeling MASH1/Achaete-scute homolog 1 with ab213151 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Nuclear staining on human medullary thyroid carcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)

Immunohistochemical analysis of paraffin-embedded human thyroid tissue labeling MASH1/Achaete-scute homolog 1 with ab213151 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Negative staining on human thyroid. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling MASH1/Achaete-scute homolog 1 with ab213151 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Negative staining on human lung. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)

Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue labeling MASH1/Achaete-scute homolog 1 with ab213151 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Negative staining on human lung squamous cell carcinoma.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue labeling MASH1/Achaete-scute homolog 1 with ab213151 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Negative staining on human lung adenocarcinoma.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)

Immunohistochemical analysis of paraffin-embedded human lung small cell carcinoma tissue labeling MASH1/Achaete-scute homolog 1 with ab213151 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Nuclear staining on human lung small cell carcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling MASH1/Achaete-scute homolog 1 with ab213151 at 1/100 (9.55 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : no staining on rat kidney.
The section was incubated with ab213151 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MASH1/Achaete-scute homolog 1 with ab213151 at 1/100 (9.55 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on rat cerebrum.
The section was incubated with ab213151 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)
  • WB

Lab

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In lane 2, the lysate was stored at -80° prior to Western Blotting. The bands beneath the target band (30 kDa) are likey to be degradation products. In lane 1, To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

All lanes:

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (ab213151) at 1/1000 dilution

All lanes:

NCI-H69 (human lung small cell carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa

false

Exposure time: 59s

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)
  • WB

Lab

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (AB213151)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : kidney, heart and spleen.
The bands beneath the target band (30 kDa) are likey to be degradation products.

ab181602 was used as a GAPDH loading control at a 1/200000 dilution.

All lanes:

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (ab213151) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Lane 7:

Rat kidney tissue lysate at 20 µg

Lane 8:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa

false

Exposure time: 59s

  • Carrier free

    Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592]

  • 660 APC

    APC Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592]

  • 578 PE

    PE Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19592

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Unsuitable for mouse IHC.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MASH1 protein also known as Achaete-Scute homolog 1 (ASCL1) plays an important role in neurogenesis. It acts as a transcription factor and regulates neuronal differentiation. MASH1 has a molecular weight of approximately 34 kDa. It expresses predominantly in the nervous system including the developing neural tube and peripheral ganglia and it can also be found in certain regions of the brain during embryonic development. ASCL1 is part of the basic helix-loop-helix (bHLH) family of transcription factors which are key regulators of neuronal identity and function.
Biological function summary

This protein regulates the expression of genes important for the development of neuronal precursors. MASH1 does not act alone; it forms heterodimers with other bHLH proteins which enhance its ability to bind DNA and influence gene transcription. It helps convert multipotent neural progenitors into committed neuronal precursors which later differentiate into specific neuronal cell types. MASH1's role is particularly important during early stages of neuronal differentiation ensuring proper formation and maturation of the nervous system.

Pathways

MASH1 is central to the Notch signaling pathway and other pathways involved in neural differentiation. Within the Notch pathway MASH1 interacts with proteins like HES1 to balance the differentiation of neural progenitor cells into neurons and glial cells. This protein also connects with other pathway components such as NEUROG2 a downstream target to promote neuronal fate specification. These interactions illustrate MASH1’s integration into complex signaling networks that govern cell fate decisions during neurogenesis.

MASH1 has significant associations with neurodevelopmental disorders and certain cancers. Aberrant ASCL1 activity links to conditions like Hirschsprung's disease where neural crest development is impaired and some types of lung cancer particularly pulmonary neuroendocrine tumors. It is known to interact with the RET protein in the context of Hirschsprung's disease and with proteins like MYC in cancer pathways highlighting its multifaceted involvement in pathology. Understanding these associations helps elucidate the mechanisms of these diseases and supports therapeutic target development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that plays a key role in neuronal differentiation : acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach (By similarity).
See full target information ASCL1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 44:282 PubMed41057919

2025

Long-term patient-derived ovarian cancer organoids closely recapitulate tumor of origin and clinical response.

Applications

Unspecified application

Species

Unspecified reactive species

Lucie Thorel,Enora Dolivet,Pierre-Marie Morice,Romane Florent,Jordane Divoux,Marion Perréard,Lucie Lecouflet,Guillaume Desmartin,Chloé Marde Alagama,Florence Giffard,Alexandra Leconte,Justine Lequesne,Bénédicte Clarisse,Mélanie Briand,Alimatou Traoré,Céline Villenet,Jean-Pascal Meneboo,Guillaume Babin,Léopold Gaichies,Sandrine Martin-Françoise,Jean-François Le Brun,Roman Rouzier,Emilie Brotin,Christophe Denoyelle,Nicolas Vigneron,Raphaël Leman,Dominique Vaur,Laurent Castera,Cécile Blanc-Fournier,Nicolas Elie,Benoit Plancoulaine,Florence Joly,Matthieu Meryet-Figuière,Martin Figeac,Louis-Bastien Weiswald,Laurent Poulain

Nature communications 16:5728 PubMed40593621

2025

HDL-bound S1P affects the subventricular niche and early neuropathological features of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Byung Jo Choi,Ju Yeon Hong,Min Hee Park,Kang Ho Park,Wan Hui Han,Hee Ji Yoon,Hye Yoon Jung,Kyung Yeol Kim,Sun Ae Lee,Eun Young Lim,Jung Woo Hur,Im-Sook Song,So Yeon Jeon,Min-Koo Choi,Christina Christoffersen,Hee-Jin Kim,Seung Hyun Kim,Edward H Schuchman,Jae-Sung Bae,Hee Kyung Jin

Nature communications 14:7613 PubMed37993461

2023

Single-cell epigenomics and spatiotemporal transcriptomics reveal human cerebellar development.

Applications

Unspecified application

Species

Unspecified reactive species

Suijuan Zhong,Mengdi Wang,Luwei Huang,Youqiao Chen,Yuxin Ge,Jiyao Zhang,Yingchao Shi,Hao Dong,Xin Zhou,Bosong Wang,Tian Lu,Xiaoxi Jing,Yufeng Lu,Junjing Zhang,Xiaoqun Wang,Qian Wu

Advanced genetics (Hoboken, N.J.) 3:2100060 PubMed36618022

2022

Single-Cell Transcriptome Identifies Drug-Resistance Signature and Immunosuppressive Microenvironment in Metastatic Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Haiping Zhang,Lele Zhang,Dianke Li,Mengfan Qi,Liping Zhang,Huansha Yu,Di Wang,Gening Jiang,Xujun Wang,Xianmin Zhu,Peng Zhang

Diagnostics (Basel, Switzerland) 10: PubMed33202998

2020

Integrated Immunohistochemical Study on Small-Cell Carcinoma of the Lung Focusing on Transcription and Co-Transcription Factors.

Applications

Unspecified application

Species

Unspecified reactive species

Younosuke Sato,Isamu Okamoto,Hiroki Kameyama,Shinji Kudoh,Haruki Saito,Mune Sanada,Noritaka Kudo,Joeji Wakimoto,Kosuke Fujino,Yuki Ikematsu,Kentaro Tanaka,Ayako Nishikawa,Ryo Sakaguchi,Takaaki Ito

Molecular neurobiology 57:997-1008 PubMed31654318

2019

Histone Methylations Define Neural Stem/Progenitor Cell Subtypes in the Mouse Subventricular Zone.

Applications

Unspecified application

Species

Unspecified reactive species

Zhichao Zhang,Adeel Manaf,Yanjiao Li,Sonia Peña Perez,Rajikala Suganthan,John Arne Dahl,Magnar Bjørås,Arne Klungland
View all publications
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