AB211327

Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840]

Name: Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) | Abcam
Brand: Abcam Limited
SKU: AB211327
Price: 335 USD
Availability: InStock
Rating: 4 (3 reviews)

BOND RX™ Validated
RabMAb
Recombinant
Advanced Validation
20ul selling size
What is this?

(3 Reviews)

(49 Publications)

Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) is a rabbit monoclonal antibody detecting MASH1/Achaete-scute homolog 1 in Western Blot, IP, IHC-P, ICC/IF, ChIP-seq. Suitable for Human, Mouse, Rat.

IHC Leica BOND RX^TM protocol - download the protocol

- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

View Alternative Names

ASH1, BHLHA46, HASH1, ASCL1, Achaete-scute homolog 1, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46

17 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemical analysis of 4% paraformaldehyde-fixed Ascl1^CreER/+; ROSA26^mTmG/+ mouse thyroid tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution, followed by Tyramide Signal Amplification (red).

Counterstain : DAPI (blue) and chick anti-GFP (green).

ROSA26^mTmG/+ mice were bred with Ascl1^CreER/+ mice, in which the Ascl1 promoter drives the expression of Cre, to generate mice carrying the genotype of Ascl1^CreER/+; ROSA26^mTmG/+. Cre activation upon Tamoxifen administration resulted in eGFP expression from the ROSA26^mTmG/+ allele. The thyroid tissue from Ascl1^CreER/+; ROSA26^mTmG/+ mouse was fixed and embedded in paraffin. Paraffin section was stained with rabbit anti-MASH1/Achaete-scute homolog 1 (ab211327) antibody (Red color) and chick anti-GFP antibody (Green color). Tyramide signal amplification was used for high-resolution imaging of low-abundance targets.

The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : no staining on human kidney.
The section was incubated with ab211327 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human lung cancer.
The section was incubated with ab211327 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemical analysis of formalin fixed paraffin embedded human small cell lung carcinoma labelling MASH1/Achaete-scute homolog 1 with ab211327 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

ab211327 Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized NCI-H69 Human small cell lung cancer cells labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution (red).

The nuclear counterstain is DAPI (blue).

Nuclear MASH1/Achaete-scute homolog 1 staining on NCI-H69 cells.

The images were kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

ChIP-seq

Lab

ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Chromatin was prepared from NCI-H69 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab211327 [EPR19840]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.

ChIP-seq

Lab

ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

ChIP-seq

Lab

ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Supplier Data

Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg of NCI-H69 (Human small cell lung cancer cell line) whole cell lysate with ab211327 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1 : NCI-H69 whole cell lysate 10 μg (Input).

Lane 2 : ab211327 IP in NCI-H69 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab211327 in NCI-H69 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

Predicted band size: 25 kDa

false

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : no staining on mouse kidney.
The section was incubated with ab211327 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemical analysis of formalin fixed paraffin embedded mouse brain labelling MASH1/Achaete-scute homolog 1 with ab211327 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a ChromoMap DAB kit and anti-rabbit HQ and anti-HQ HRP detection. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on rat cerebrum.
The section was incubated with ab211327 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : no staining on rat kidney.
The section was incubated with ab211327 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemical analysis of 4% paraformaldehyde-fixed mouse small cell lung cancer section labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution, followed by Donkey anti Rabbit Alexa-594 at 1/500 dilution (red).

Nuclear counterstain : DAPI (blue).

Positive staining of MASH1/Achaete-scute homolog 1 on mouse small cell lung cancer.

The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on mouse cerebrum.
The section was incubated with ab211327 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg of mouse medullary thyroid cancer lysate with ab211327 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1 : Mouse medullary thyroid cancer lysate 10 μg (Input).

Lane 2 : ab211327 IP in mouse medullary thyroid cancer lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab211327 in mouse medullary thyroid cancer lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

Predicted band size: 25 kDa

false

Supplier Data

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 15 seconds; Lane 2 : 10 seconds

Ascl1 is highly expressed in medullary thyroid cancer and small cell lung cancer (PMID : 17090547 and 25267614). Our results showed specific band on mouse medullary thyroid cancer lysate and NCI-H69 (Human small cell lung cancer) lysate. The lysates were kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

All lanes:

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) at 1/1000 dilution

Lane 1:

Mouse medullary thyroid cancer lysate at 20 µg

Lane 2:

NCI-H69 (Human small cell lung cancer cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 25 kDa

Observed band size: 30 kDa

false

Carrier free

Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19840

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

ChIP-seq, IP, WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The fluorescence IHC images were provided by a collaborator. Our in-house testing failed in fluorescence IHC. We do not guarantee fluorescence IHC.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "8 µg for 10^7 Cells", "ChIPseq-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" } } }

style

left-column

Product details

Product Specifications

Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIP-seq, ICC/IF, IHC-P, IP, WB in human, mouse, rat samples.
Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) specifically detects MASH1/Achaete-scute homolog 1 (UniProt ID: Q02067; Molecular weight: 25kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) has been cited over 21 times in peer reviewed journals and is trusted by the scientific community.

Related Products
Conjugation-ready, carrier free format available for antibody clone EPR19840 - ab240385.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MASH1 protein also known as Achaete-Scute homolog 1 (ASCL1) plays an important role in neurogenesis. It acts as a transcription factor and regulates neuronal differentiation. MASH1 has a molecular weight of approximately 34 kDa. It expresses predominantly in the nervous system including the developing neural tube and peripheral ganglia and it can also be found in certain regions of the brain during embryonic development. ASCL1 is part of the basic helix-loop-helix (bHLH) family of transcription factors which are key regulators of neuronal identity and function.

Biological function summary

This protein regulates the expression of genes important for the development of neuronal precursors. MASH1 does not act alone; it forms heterodimers with other bHLH proteins which enhance its ability to bind DNA and influence gene transcription. It helps convert multipotent neural progenitors into committed neuronal precursors which later differentiate into specific neuronal cell types. MASH1's role is particularly important during early stages of neuronal differentiation ensuring proper formation and maturation of the nervous system.

Pathways

MASH1 is central to the Notch signaling pathway and other pathways involved in neural differentiation. Within the Notch pathway MASH1 interacts with proteins like HES1 to balance the differentiation of neural progenitor cells into neurons and glial cells. This protein also connects with other pathway components such as NEUROG2 a downstream target to promote neuronal fate specification. These interactions illustrate MASH1’s integration into complex signaling networks that govern cell fate decisions during neurogenesis.

MASH1 has significant associations with neurodevelopmental disorders and certain cancers. Aberrant ASCL1 activity links to conditions like Hirschsprung's disease where neural crest development is impaired and some types of lung cancer particularly pulmonary neuroendocrine tumors. It is known to interact with the RET protein in the context of Hirschsprung's disease and with proteins like MYC in cancer pathways highlighting its multifaceted involvement in pathology. Understanding these associations helps elucidate the mechanisms of these diseases and supports therapeutic target development.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

style

left-column

Target data

Transcription factor that plays a key role in neuronal differentiation : acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach (By similarity).

See full target information ASCL1

style

left-column

Publications (49)

Recent publications for all applications. Explore the full list and refine your search

Nature 647:257-267 PubMed40963028

2025

Basal cell of origin resolves neuroendocrine-tuft lineage plasticity in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Abbie S Ireland,Daniel A Xie,Sarah B Hawgood,Margaret W Barbier,Lisa Y Zuo,Benjamin E Hanna,Scarlett Lucas-Randolph,Darren R Tyson,Benjamin L Witt,Ramaswamy Govindan,Afshin Dowlati,Justin C Moser,Anish Thomas,Sonam Puri,Charles M Rudin,Joseph M Chan,Andrew Elliott,Trudy G Oliver

Molecular medicine (Cambridge, Mass.) 31:232 PubMed40500731

2025

ONECUT2 reprograms neuroendocrine fate and is an actionable therapeutic target in small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mirian Gutiérrez,Irene Zamora,Raquel Iriarte,María José Pajares,Qian Yang,Chen Qian,Nerea Otegui,Joaquín Fernández-Irigoyen,Enrique Santamaría,Nicolas Alcala,Alexandra Sexton-Oates,Lynnette Fernández-Cuesta,Miguel Barajas,Alfonso Calvo,Luis M Montuenga,Beatrice Knudsen,Sungyong You,Michael R Freeman,Ignacio Encío,Mirja Rotinen

Nature communications 16:4865 PubMed40419484

2025

FOXA2 promotes metastatic competence in small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kenta Kawasaki,Sohrab Salehi,Yingqian A Zhan,Kevin Chen,Jun Ho Lee,Eralda Salataj,Hong Zhong,Parvathy Manoj,Dennis Kinyua,Barbara P Mello,Harsha Sridhar,Sam E Tischfield,Irina Linkov,Nicholas Ceglia,Matthew Zatzman,Eliyahu Havasov,Neil J Shah,Fanli Meng,Brian Loomis,Umesh K Bhanot,Esther Redin,Elisa de Stanchina,Pierre-Jacques Hamard,Richard P Koche,Andrew McPherson,Álvaro Quintanal-Villalonga,Sohrab P Shah,Joan Massagué,Charles M Rudin

Cancer immunology, immunotherapy : CII 74:213 PubMed40402312

2025

Molecular subtyping dictates therapeutic response to anti-PD-L1 immunotherapy in ES-SCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Zhang,Guoxin Wang,Wenjie Yan,Dong Wang,Jie Yin,Yong Song,Mingxiang Ye,Tangfeng Lv

eLife 14: PubMed40388211

2025

Viral-mediated Pou5f1 (Oct4) overexpression and inhibition of Notch signaling synergistically induce neurogenic competence in mammalian Müller glia.

Applications

Unspecified application

Species

Unspecified reactive species

Nguyet Le,Sherine Awad,Isabella Palazzo,Thanh Hoang,Seth Blackshaw

Cell reports 44:115597 PubMed40257863

2025

Requirements for the neurodevelopmental disorder-associated gene ZNF292 in human cortical interneuron development and function.

Applications

Unspecified application

Species

Unspecified reactive species

Komal Kaushik,Gareth Chapman,Ramachandran Prakasam,Faiza Batool,Maamoon Saleh,Julianna Determan,James E Huettner,Kristen L Kroll

Cells 14: PubMed40136675

2025

Lateral Ventricular Neural Stem Cells Provide Negative Feedback to Circuit Activation Through GABAergic Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Moawiah M Naffaa,Henry H Yin

EBioMedicine 112:105515 PubMed39808946

2025

ATAD2 is a potential immunotherapy target for patients with small cell lung cancer harboring HLA-A∗0201.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yuan,Sini Li,Yixiang Zhu,Lin Yang,Xue Zhang,Yan Qu,Zhijie Wang,Jianchun Duan,Jia Zhong,Yanhua Tian,Lihui Liu,Boyang Sun,Kailun Fei,Zheng Liu,Jian Zhang,Yan He,Yufeng Guo,DanMing He,Wei Zhuang,Jinsong Zhang,Zixiao Ma,Hua Bai,Jie Wang

Proceedings of the National Academy of Sciences of the United States of America 121:e2405001121 PubMed39361648

2024

Enhancer landscape of lung neuroendocrine tumors reveals regulatory and developmental signatures with potential theranostic implications.

Applications

Unspecified application

Species

Unspecified reactive species

Ester Davis,Shani Avniel-Polak,Shahd Abu-Kamel,Israel Antman,Tsipora Saadoun,Chava Brim,Mohammad Jumaa,Yariv Maron,Ofra Maimon,Anat Bel-Ange,Karine Atlan,Tomer Tzur,Firas Abu Akar,Ori Wald,Uzi Izhar,Merav Hecht,Simona Grozinsky-Glasberg,Yotam Drier

Molecular cancer therapeutics 23:1789-1800 PubMed39295275

2024

ASCL1 Restrains ERK1/2 to Promote Survival of a Subset of Neuroendocrine Lung Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Martin-Vega,Svetlana A Earnest,Alexander Augustyn,Chonlarat Wichaidit,Luc Girard,Michael Peyton,John D Minna,Jane E Johnson,Melanie H Cobb

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (49)

Product promise