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Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] is a rabbit recombinant monoclonal antibody that is used to detect MASH1/Achaete-scute homolog 1 in ChIP-seq, ICC/IF, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rat samples.

- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR19840 is cited in over 40 publications


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327), expandable thumbnail
  • Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327), expandable thumbnail
  • Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFChIP-seq
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Tested
Expected
Expected
Expected
Rat
Tested
Expected
Not recommended
Expected
Expected

Tested
Tested

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
8 µg for 107 Cells
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach (By similarity).

Alternative names

Recommended products

Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] is a rabbit recombinant monoclonal antibody that is used to detect MASH1/Achaete-scute homolog 1 in ChIP-seq, ICC/IF, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rat samples.

- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR19840 is cited in over 40 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19840
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Product Specifications

Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIP-seq, ICC/IF, IHC-P, IP, WB in human, mouse, rat samples.
Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) specifically detects MASH1/Achaete-scute homolog 1 (UniProt ID: Q02067; Molecular weight: 25kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) has been cited over 21 times in peer reviewed journals and is trusted by the scientific community.


Related Products
Conjugation-ready, carrier free format available for antibody clone EPR19840 - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free ab240385.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The MASH1 protein also known as Achaete-Scute homolog 1 (ASCL1) plays an important role in neurogenesis. It acts as a transcription factor and regulates neuronal differentiation. MASH1 has a molecular weight of approximately 34 kDa. It expresses predominantly in the nervous system including the developing neural tube and peripheral ganglia and it can also be found in certain regions of the brain during embryonic development. ASCL1 is part of the basic helix-loop-helix (bHLH) family of transcription factors which are key regulators of neuronal identity and function.

Biological function summary

This protein regulates the expression of genes important for the development of neuronal precursors. MASH1 does not act alone; it forms heterodimers with other bHLH proteins which enhance its ability to bind DNA and influence gene transcription. It helps convert multipotent neural progenitors into committed neuronal precursors which later differentiate into specific neuronal cell types. MASH1's role is particularly important during early stages of neuronal differentiation ensuring proper formation and maturation of the nervous system.

Pathways

MASH1 is central to the Notch signaling pathway and other pathways involved in neural differentiation. Within the Notch pathway MASH1 interacts with proteins like HES1 to balance the differentiation of neural progenitor cells into neurons and glial cells. This protein also connects with other pathway components such as NEUROG2 a downstream target to promote neuronal fate specification. These interactions illustrate MASH1’s integration into complex signaling networks that govern cell fate decisions during neurogenesis.

Associated diseases and disorders

MASH1 has significant associations with neurodevelopmental disorders and certain cancers. Aberrant ASCL1 activity links to conditions like Hirschsprung's disease where neural crest development is impaired and some types of lung cancer particularly pulmonary neuroendocrine tumors. It is known to interact with the RET protein in the context of Hirschsprung's disease and with proteins like MYC in cancer pathways highlighting its multifaceted involvement in pathology. Understanding these associations helps elucidate the mechanisms of these diseases and supports therapeutic target development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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15 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed Ascl1CreER/+; ROSA26mTmG/+ mouse thyroid tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution, followed by Tyramide Signal Amplification (red).

    Counterstain: DAPI (blue) and chick anti-GFP (green).

    ROSA26mTmG/+ mice were bred with Ascl1CreER/+ mice, in which the Ascl1 promoter drives the expression of Cre, to generate mice carrying the genotype of Ascl1CreER/+; ROSA26mTmG/+. Cre activation upon Tamoxifen administration resulted in eGFP expression from the ROSA26mTmG/+ allele. The thyroid tissue from Ascl1CreER/+; ROSA26mTmG/+ mouse was fixed and embedded in paraffin. Paraffin section was stained with rabbit anti-MASH1/Achaete-scute homolog 1 (ab211327) antibody (Red color) and chick anti-GFP antibody (Green color). Tyramide signal amplification was used for high-resolution imaging of low-abundance targets.

    The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed mouse small cell lung cancer section labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution, followed by Donkey anti Rabbit Alexa-594 at 1/500 dilution (red).

    Nuclear counterstain: DAPI (blue).

    Positive staining of MASH1/Achaete-scute homolog 1 on mouse small cell lung cancer.

    The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail

    Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2: 10 seconds

    Ascl1 is highly expressed in medullary thyroid cancer and small cell lung cancer (PMID: 17090547 and 25267614). Our results showed specific band on mouse medullary thyroid cancer lysate and NCI-H69 (Human small cell lung cancer) lysate. The lysates were kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

    All lanes: Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) at 1/1000 dilution

    Lane 1: Mouse medullary thyroid cancer lysate at 20 µg

    Lane 2: NCI-H69 (Human small cell lung cancer cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 25 kDa

    Observed band size: 30 kDa

  • Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail

    Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg of mouse medullary thyroid cancer lysate with ab211327 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution

    Lane 1: Mouse medullary thyroid cancer lysate 10 μg (Input).

    Lane 2: ab211327 IP in mouse medullary thyroid cancer lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab211327 in mouse medullary thyroid cancer lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    Predicted band size: 25 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized NCI-H69 Human small cell lung cancer cells labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution (red).

    The nuclear counterstain is DAPI (blue).

    Nuclear MASH1/Achaete-scute homolog 1 staining on NCI-H69 cells.

    The images were kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

  • Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail

    Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg of NCI-H69 (Human small cell lung cancer cell line) whole cell lysate with ab211327 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution

    Lane 1: NCI-H69 whole cell lysate 10 μg (Input).

    Lane 2: ab211327 IP in NCI-H69 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab211327 in NCI-H69 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    Predicted band size: 25 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Negative control: no staining on rat kidney.
    The section was incubated with ab211327 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail

    ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    Chromatin was prepared from NCI-H69 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab211327 [EPR19840]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Negative control: no staining on human kidney.
    The section was incubated with ab211327 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail

    ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    Chromatin was prepared from NCI-H69 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab211327 [EPR19840]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.

  • ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail

    ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    Chromatin was prepared from NCI-H69 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab211327 [EPR19840]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on rat cerebrum.
    The section was incubated with ab211327 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on mouse cerebrum.
    The section was incubated with ab211327 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on human lung cancer.
    The section was incubated with ab211327 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Negative control: no staining on mouse kidney.
    The section was incubated with ab211327 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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