Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] is a rabbit recombinant monoclonal antibody that is used to detect MASH1/Achaete-scute homolog 1 in ChIP-seq, ICC/IF, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rat samples.
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR19840 is cited in over 40 publications
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25007.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34870.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)")
![Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--western-blot-img74160.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)")
![Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunoprecipitation-img60163.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)")
![Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunocytochemistry-immunofluorescence-img59943.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)")
Publications
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- Cells 12:2023c-Cbl Regulates Murine Subventricular Zone-Derived Neural Progenitor Cells in Dependence of the Epidermal Growth Factor Receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaximilian Vogt,Madhukrishna Kolothara Unnikrishnan,Nora Heinig,Ulrike Schumann,Mirko H H Schmidt,Kathrin BarthPubMed 37830613
- International journal of molecular sciences 24:2023Skin Wound following Irradiation Aggravates Radiation-Induced Brain Injury in a Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMang Xiao,Xianghong Li,Li Wang,Bin Lin,Min Zhai,Lisa Hull,Alex Zizzo,Wanchang Cui,Juliann G KiangPubMed 37445879
- EBioMedicine 90:1045312023New AAV tools fail to detect Neurod1-mediated neuronal conversion of Müller glia and astrocytes in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe Xie,Jing Zhou,Lei-Lei Wang,Chun-Li Zhang,Bo ChenPubMed 36947961
- Signal transduction and targeted therapy 7:3462022Single-cell transcriptomic profiling reveals the tumor heterogeneity of small-cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanhua Tian,Qingqing Li,Zhenlin Yang,Shu Zhang,Jiachen Xu,Zhijie Wang,Hua Bai,Jianchun Duan,Bo Zheng,Wen Li,Yueli Cui,Xin Wang,Rui Wan,Kailun Fei,Jia Zhong,Shugeng Gao,Jie He,Carl M Gay,Jianjun Zhang,Jie Wang,Fuchou TangPubMed 36195615
- Frontiers in cell and developmental biology 10:9425792022The proneural transcription factor ASCL1 regulates cell proliferation and primes for differentiation in neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLydia M Parkinson,Sarah L Gillen,Laura M Woods,Lewis Chaytor,Daniel Marcos,Fahad R Ali,Jason S Carroll,Anna PhilpottPubMed 36263020
- Nature cancer 3:1071-10872022Ectopic JAK-STAT activation enables the transition to a stem-like and multilineage state conferring AR-targeted therapy resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSu Deng,Choushi Wang,Yunguan Wang,Yaru Xu,Xiaoling Li,Nickolas A Johnson,Atreyi Mukherji,U-Ging Lo,Lingfan Xu,Julisa Gonzalez,Lauren A Metang,Jianfeng Ye,Carla Rodriguez Tirado,Kathia Rodarte,Yinglu Zhou,Zhiqun Xie,Carlos Arana,Valli Annamalai,Xihui Liu,Donald J Vander Griend,Douglas Strand,Jer-Tsong Hsieh,Bo Li,Ganesh Raj,Tao Wang,Ping MuPubMed 36065066
- Nature communications 13:49982022Regulation of neuroendocrine plasticity by the RNA-binding protein ZFP36L1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHsiao-Yun Chen,Yavuz T Durmaz,Yixiang Li,Amin H Sabet,Amir Vajdi,Thomas Denize,Emily Walton,Yasmin Nabil Laimon,John G Doench,Navin R Mahadevan,Julie-Aurore Losman,David A Barbie,Michael Y Tolstorukov,Charles M Rudin,Triparna Sen,Sabina Signoretti,Matthew G OserPubMed 36008402
- Thoracic cancer 13:1184-11922022High mRNA expression of POU2F3 in small cell lung cancer cell lines predicts the effect of lurbinectedin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShinji Matsui,Tomohiro Haruki,Yuki Oshima,Yoshiteru Kidokoro,Tomohiko Sakabe,Yoshihisa Umekita,Hiroshige NakamuraPubMed 35278040
- Scientific reports 12:23412022ASCL1 phosphorylation and ID2 upregulation are roadblocks to glioblastoma stem cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoberta Azzarelli,Aoibheann McNally,Claudia Dell'Amico,Marco Onorati,Benjamin Simons,Anna PhilpottPubMed 35149717
- Cell proliferation 55:e132012022In vivo development and single-cell transcriptome profiling of human brain organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShichao Huang,Fei Huang,Huiying Zhang,Yongfeng Yang,Juan Lu,Jiadong Chen,Li Shen,Gang PeiPubMed 35141969
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | ChIP-seq | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected | Expected | Expected |
| Rat | Tested | Expected | Not recommended | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Species Human | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ChIP-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 8 µg for 107 Cells | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach (By similarity).
Alternative names
ASH1, BHLHA46, HASH1, ASCL1, Achaete-scute homolog 1, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46
Recommended products
Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] is a rabbit recombinant monoclonal antibody that is used to detect MASH1/Achaete-scute homolog 1 in ChIP-seq, ICC/IF, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rat samples.
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR19840 is cited in over 40 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR19840
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIP-seq, ICC/IF, IHC-P, IP, WB in human, mouse, rat samples.
Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) specifically detects MASH1/Achaete-scute homolog 1 (UniProt ID: Q02067; Molecular weight: 25kDa) and is sold in 100 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) has been cited over 21 times in peer reviewed journals and is trusted by the scientific community.
Related Products
Conjugation-ready, carrier free format available for antibody clone EPR19840 - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free ab240385.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MASH1 protein also known as Achaete-Scute homolog 1 (ASCL1) plays an important role in neurogenesis. It acts as a transcription factor and regulates neuronal differentiation. MASH1 has a molecular weight of approximately 34 kDa. It expresses predominantly in the nervous system including the developing neural tube and peripheral ganglia and it can also be found in certain regions of the brain during embryonic development. ASCL1 is part of the basic helix-loop-helix (bHLH) family of transcription factors which are key regulators of neuronal identity and function.
- Biological function summary
This protein regulates the expression of genes important for the development of neuronal precursors. MASH1 does not act alone; it forms heterodimers with other bHLH proteins which enhance its ability to bind DNA and influence gene transcription. It helps convert multipotent neural progenitors into committed neuronal precursors which later differentiate into specific neuronal cell types. MASH1's role is particularly important during early stages of neuronal differentiation ensuring proper formation and maturation of the nervous system.
- Pathways
MASH1 is central to the Notch signaling pathway and other pathways involved in neural differentiation. Within the Notch pathway MASH1 interacts with proteins like HES1 to balance the differentiation of neural progenitor cells into neurons and glial cells. This protein also connects with other pathway components such as NEUROG2 a downstream target to promote neuronal fate specification. These interactions illustrate MASH1’s integration into complex signaling networks that govern cell fate decisions during neurogenesis.
- Associated diseases and disorders
MASH1 has significant associations with neurodevelopmental disorders and certain cancers. Aberrant ASCL1 activity links to conditions like Hirschsprung's disease where neural crest development is impaired and some types of lung cancer particularly pulmonary neuroendocrine tumors. It is known to interact with the RET protein in the context of Hirschsprung's disease and with proteins like MYC in cancer pathways highlighting its multifaceted involvement in pathology. Understanding these associations helps elucidate the mechanisms of these diseases and supports therapeutic target development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
15 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25007.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Immunohistochemical analysis of 4% paraformaldehyde-fixed Ascl1CreER/+; ROSA26mTmG/+ mouse thyroid tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution, followed by Tyramide Signal Amplification (red).
Counterstain: DAPI (blue) and chick anti-GFP (green).
ROSA26mTmG/+ mice were bred with Ascl1CreER/+ mice, in which the Ascl1 promoter drives the expression of Cre, to generate mice carrying the genotype of Ascl1CreER/+; ROSA26mTmG/+. Cre activation upon Tamoxifen administration resulted in eGFP expression from the ROSA26mTmG/+ allele. The thyroid tissue from Ascl1CreER/+; ROSA26mTmG/+ mouse was fixed and embedded in paraffin. Paraffin section was stained with rabbit anti-MASH1/Achaete-scute homolog 1 (ab211327) antibody (Red color) and chick anti-GFP antibody (Green color). Tyramide signal amplification was used for high-resolution imaging of low-abundance targets.
The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34870.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Immunohistochemical analysis of 4% paraformaldehyde-fixed mouse small cell lung cancer section labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution, followed by Donkey anti Rabbit Alexa-594 at 1/500 dilution (red).
Nuclear counterstain: DAPI (blue).
Positive staining of MASH1/Achaete-scute homolog 1 on mouse small cell lung cancer.
The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--western-blot-img74160.jpg "Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 15 seconds; Lane 2: 10 seconds
Ascl1 is highly expressed in medullary thyroid cancer and small cell lung cancer (PMID: 17090547 and 25267614). Our results showed specific band on mouse medullary thyroid cancer lysate and NCI-H69 (Human small cell lung cancer) lysate. The lysates were kindly provided by our collaborator Dr. Hai Song, Zhejiang University.
All lanes: Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) at 1/1000 dilution
Lane 1: Mouse medullary thyroid cancer lysate at 20 µg
Lane 2: NCI-H69 (Human small cell lung cancer cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 25 kDa
Observed band size: 30 kDa
![Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunoprecipitation-img60163.jpg "Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg of mouse medullary thyroid cancer lysate with ab211327 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution
Lane 1: Mouse medullary thyroid cancer lysate 10 μg (Input).
Lane 2: ab211327 IP in mouse medullary thyroid cancer lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab211327 in mouse medullary thyroid cancer lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Predicted band size: 25 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunocytochemistry-immunofluorescence-img59943.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized NCI-H69 Human small cell lung cancer cells labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution (red).
The nuclear counterstain is DAPI (blue).
Nuclear MASH1/Achaete-scute homolog 1 staining on NCI-H69 cells.
The images were kindly provided by our collaborator Dr. Hai Song, Zhejiang University.
![Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunoprecipitation-img10789.jpg "Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg of NCI-H69 (Human small cell lung cancer cell line) whole cell lysate with ab211327 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution
Lane 1: NCI-H69 whole cell lysate 10 μg (Input).
Lane 2: ab211327 IP in NCI-H69 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab211327 in NCI-H69 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Predicted band size: 25 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440799.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: no staining on rat kidney.
The section was incubated with ab211327 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with hematoxylin.Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--chip-sequencing-img439385.jpg "ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Chromatin was prepared from NCI-H69 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab211327 [EPR19840]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440797.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: no staining on human kidney.
The section was incubated with ab211327 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with hematoxylin.Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--chip-sequencing-img439383.jpg "ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Chromatin was prepared from NCI-H69 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab211327 [EPR19840]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.
![ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--chip-sequencing-img439384.jpg "ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Chromatin was prepared from NCI-H69 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab211327 [EPR19840]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440796.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on rat cerebrum.
The section was incubated with ab211327 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with hematoxylin.Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440795.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on mouse cerebrum.
The section was incubated with ab211327 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with hematoxylin.Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440794.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human lung cancer.
The section was incubated with ab211327 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with hematoxylin.Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440798.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 (5.1 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: no staining on mouse kidney.
The section was incubated with ab211327 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with hematoxylin.Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunoprecipitation-img10789.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440799.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
![ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--chip-sequencing-img439385.jpg/_jcr_content/renditions/webp-475.webp "ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440797.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
![ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--chip-sequencing-img439383.jpg/_jcr_content/renditions/webp-475.webp "ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
![ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--chip-sequencing-img439384.jpg/_jcr_content/renditions/webp-475.webp "ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440796.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440795.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440794.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327), expandable thumbnail](https://content.abcam.com/products/images/mash1-achaete-scute-homolog-1-antibody-epr19840-ab211327--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440798.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
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