Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665)

Rabbit Recombinant Multiclonal MASH1/Achaete-scute homolog 1 antibody. Suitable for WB, ChIP-seq, IHC-P, IP and reacts with Human, Mouse, Rat samples.

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Images

ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	ChIP-seq	IHC-P	IP	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Not recommended	Not recommended
Mouse	Tested	Expected	Not recommended	Not recommended	Not recommended	Not recommended
Rat	Tested	Expected	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 8 µg for 10⁷ Cells	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Target data

See full target information ASCL1

Function

Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach (By similarity).

Additional Targets

Ascl1

Alternative names

ASH1, BHLHA46, HASH1, ASCL1, Achaete-scute homolog 1, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46

Rabbit Recombinant Multiclonal MASH1/Achaete-scute homolog 1 antibody. Suitable for WB, ChIP-seq, IHC-P, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Clone number: RM1182
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for mouse IHC-P, mouse/rat IP.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665)
Chromatin was prepared from NCI-H69 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab322665 [RM1182]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665)
Chromatin was prepared from NCI-H69 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab322665 [RM1182]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665)
Chromatin was prepared from NCI-H69 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab322665 [RM1182]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665)
MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg NCI-H69 (human lung small cell carcinoma cell line) whole cell lysate with ab322665 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322665 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NCI-H69 (human lung small cell carcinoma cell line) whole cell lysate

Lane 2: ab322665 IP in NCI-H69 (human lung small cell carcinoma cell line) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab322665 in NCI-H69 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST
All lanes: Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665) at 1/30 dilution
All lanes: NCI-H69 (human lung small cell carcinoma cell line) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 76s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MASH1/Achaete-scute homolog 1 with ab322665 at 1/50 (10.42 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: no staining on human kidney. The section was incubated with ab322665 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MASH1/Achaete-scute homolog 1 with ab322665 at 1/100 (5.21 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on rat cerebrum. The section was incubated with ab322665 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665)
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling MASH1/Achaete-scute homolog 1 with ab322665 at 1/50 (10.42 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human thyroid carcinoma. The section was incubated with ab322665 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665)
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling MASH1/Achaete-scute homolog 1 with ab322665 at 1/50 (10.42 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human lung carcinoma. The section was incubated with ab322665 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: kidney
The bands beneath the target band (30 kDa) are likey to be degradation products.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665) at 1/1000 dilution
Lane 1: Human brain tissue lysate at 20 µg
Lane 2: Human kidney tissue lysate at 20 µg
Lane 3: Mouse brain tissue lysate at 20 µg
Lane 4: Mouse kidney tissue lysate at 20 µg
Lane 5: Rat brain tissue lysate at 20 µg
Lane 6: Rat kidney tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 30 kDa, 36 kDa
Exposure time: 180s
Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In lane 2, the lysate was stored at -80℃ prior to Western Blotting. The bands beneath the target band (30 kDa) are likey to be degradation products. In lane 1/3, To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
Exposure time: Lanes 1-2: 180 seconds; Lane 3: 59 seconds
All lanes: Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665) at 1/1000 dilution
Lanes 1 - 2: NCI-H69 (human lung small cell carcinoma cell line) whole cell lysate at 20 µg
Lane 3: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 30 kDa
Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: A549 (PMID: 31231131).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665) at 1/1000 dilution
Lane 1: NCI-H69 (human lung small cell carcinoma cell line) whole cell lysate at 20 µg
Lane 2: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 30 kDa, 36 kDa
Exposure time: 180s