Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (AB277528)

Fluorescence multiplex immunohistochemical analysis of human liver (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-MASP2 stained on cytoplasm of hepatocytes (ab277520; gray; Opal™690) at 1 : 100 ( 5.22 μg/ml) [Panel B] , anti-CD163 stained on Kupffer cells (ab213612; green; Opal™520) at 1 : 8000 ( 0.13 μg/ml) [Panel C], and anti-eNOS tained on endothelial cells (ab252439; red; Opal™570) at 1 : 200 ( 3.005 μg/ml) [Panel D] on human liver. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining : in the order of ab277520, ab213612, and ab252439 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. This data was developed using ab277520, the same antibody clone in a different buffer formulation.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (AB277528)

This data was developed using ab277520, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling MASP2 with ab277520 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human liver (PMID : 16395704). Counter stained with Hematoxylin. The section was incubated with ab277520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (AB277528)

This data was developed using ab277520, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular weight is consistent with what has been described in the literature (PMID : 16116205, 23785123, 21871896, 15078867).

Negative control : A549 (PMID : 17131120).
Low expression : HepG2 (PMID : 17131120).

Exposure times : Lanes 1-3, 15 seconds;

Lane 4 : 180 seconds.

All lanes:

Western blot - Anti-MASP2 antibody [EPR23588-44] (<a href='/en-us/products/primary-antibodies/masp2-antibody-epr23588-44-ab277520'>ab277520</a>) at 1/1000 dilution

Lane 1:

Human plasma at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

A549 (Human lung carcinoma epithelial cell ), whole cell lysate at 20 µg

Lane 4:

Human kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 76 kDa

Observed band size: 19 kDa,50 kDa,75 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (AB277528)

This data was developed using ab277520, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling MASP2 with ab277520 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human kidney (PMID : 30286468). Counter stained with Hematoxylin. The section was incubated with ab277520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (AB277528)

This data was developed using ab277520, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MASP2 with ab277520 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on plasma of human colon (PMID : 32771488). Counter stained with Hematoxylin. The section was incubated with ab277520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (AB277528)

This data was developed using ab277520, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling MASP2 with ab277520 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse liver. Counter stained with Hematoxylin. The section was incubated with ab277520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (AB277528)

This data was developed using ab277520, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling MASP2 with ab277520 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat liver. Counter stained with Hematoxylin. The section was incubated with ab277520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.