Anti-MASPIN antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASPIN antibody (AB182785)

Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ab182785 at dilution 1/60,on the right is trested with the fusion protein

• WB

Lab

Western blot - Anti-MASPIN antibody (AB182785)

Lanes 1 - 4 : Merged signal (red and green). Green - ab182785 observed at 42 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab182785 was shown to react with MASPIN in wild-type cells in Western blot with loss of signal observed in SERPINB5 knockout cell line ab264750 (SERPINB5 knockout cell lysate ab258659). Wild-type and SERPINB5 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with ab182785 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1/1000 dilution and a 1/20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-MASPIN antibody (ab182785) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

SERPINB5 knockout HeLa cell lysate at 20 µg

Lane 3:

HaCaT cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

• WB

Supplier Data

Western blot - Anti-MASPIN antibody (AB182785)

All lanes:

Western blot - Anti-MASPIN antibody (ab182785) at 1/1000 dilution

Lane 1:

Mouse esophagus tissue lysate at 40 µg

Lane 2:

Human cervical cancer tissue lysate at 40 µg

Secondary

All lanes:

Goat anti Rabbit IgG at 1/10000 dilution

Predicted band size: 42 kDa

false

Exposure time: 13s

• WB

CiteAb

Western blot - Anti-MASPIN antibody (AB182785)

Western Blotting using Anti-MASPIN antibody, ab182785. Publication image from Zhang, P. et al., 2020, J Biomed Sci, 32005234. Legend direct from paper.

SERPINB5 is indispensable for TRIM21-mediated GMPS–TP53 repression after radiation. a Co-immunoprecipitation following western blotting in NPC cells with SERPINB5–HA and TRIM21–MYC overexpression. b Immunofluorescence staining analysis of SERPINB5 and TRIM21 in NPC cells. c Western blot detection of SERPINB5 expression in normal NP69 cell line and in NPC cell lines. d Western blot detection of GMPS and TP53 in NPC cells with TRIM21 GOF or SERPINB5 LOF. e Co-immunoprecipitation using anti-HA antibody in NPC cells with SERPINB5–HA and GMPS–FLAG overexpression. f Immunofluorescence staining to detect the co-localization of GMPS and SERPINB5 in NPC cells with or without ionizing radiation. g GMPS expression in immune-purified protein by anti-HA antibody from NPC cells with or without TRIM21 LOF. h GMPS expression in immune-purified protein by anti-MYC antibody from NPC cells with or without SERPINB5 LOF. i GMPS expression in NPC cells with TRIM21 or SERPINB5 overexpression. j Immunofluorescence staining of overexpressed GMPS and ubiquitin in HONE1 cells with or without ionizing radiation. The localization of GMPS and was evaluated in condition of SERPINB5 or TRIM21 LOF. For the co-immunoprecipitation assay, MG132 was added into the cell medium before cell harvesting to avoid GMPS degradation. *P < 0.05, **P < 0.01, ***P < 0.001

false