AB216481

Anti-MASPIN antibody

Name: Anti-MASPIN antibody (ab216481) | Abcam
Brand: Abcam Limited
SKU: AB216481

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(1 Publication)

Rabbit Polyclonal MASPIN antibody. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Serpinb5 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

PI5, SERPINB5, Serpin B5, Maspin, Peptidase inhibitor 5, PI-5

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASPIN antibody (AB216481)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASPIN antibody (AB216481)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon carcinoma tissue, labeling MASPIN using ab216481 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASPIN antibody (AB216481)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rat rectum tissue, labeling MASPIN using ab216481 at a 1/500 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Serpinb5 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P70124

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MASPIN also known as SERPINB5 is a non-inhibitory serine protease inhibitor with a mass of approximately 42 kDa. It functions mechanically to regulate cell adhesion migration apoptosis and angiogenesis. MASPIN localizes mainly in the cytoplasm and nucleus and expresses predominantly in epithelial cells including those in the mammary gland prostate and keratinocytes in the skin. It acts as a tumor suppressor and researchers often use immunohistochemistry (IHC) to study its expression patterns in various tissues.

Biological function summary

MASPIN influences apoptosis and cell motility playing a critical role in tumor suppression. It does not form part of a complex but interacts directly with extracellular matrix components. MASPIN can inhibit angiogenesis by reducing the proliferation and migration of endothelial cells. Scientists have noted its role in maintaining normal cell architecture and inhibiting tumor cell invasion.

Pathways

Researchers place MASPIN in important signaling networks impacting cancer progression and metastasis. It participates in the PI3K/AKT pathway thereby influencing cell growth and survival. MASPIN also intersects with TGF-β signaling and related proteins like SMAD which mediate growth arrest and apoptosis. By altering these pathways MASPIN helps modulate responses to cellular stress and inhibit lethal growth in cancer cells.

MASPIN often associates with breast and prostate cancers. Its expression inversely correlates with tumor progression suggesting a protective role. MASPIN's interaction with proteins like p53 highlights its contribution to tumor suppression. Abnormal MASPIN presence links to aggressive disease phenotypes making it a potential biomarker for prognostic evaluation in oncology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

See full target information SERPINB5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 41:1911-1926.e8 PubMed37802053

2023

TIM-3 blockade in diffuse intrinsic pontine glioma models promotes tumor regression and antitumor immune memory.

Applications

Unspecified application

Species

Unspecified reactive species

Iker Ausejo-Mauleon,Sara Labiano,Daniel de la Nava,Virginia Laspidea,Marta Zalacain,Lucía Marrodán,Marc García-Moure,Marisol González-Huarriz,Irati Hervás-Corpión,Laasya Dhandapani,Silvestre Vicent,Maria Collantes,Iván Peñuelas,Oren J Becher,Mariella G Filbin,Li Jiang,Jenna Labelle,Carlos A O de Biagi-Junior,Javad Nazarian,Sandra Laternser,Timothy N Phoenix,Jasper van der Lugt,Mariette Kranendonk,Raoull Hoogendijk,Sabine Mueller,Carlos De Andrea,Ana C Anderson,Elizabeth Guruceaga,Carl Koschmann,Viveka Nand Yadav,Jaime Gállego Pérez-Larraya,Ana Patiño-García,Fernando Pastor,Marta M Alonso

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MASPIN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASPIN antibody (AB216481)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASPIN antibody (AB216481)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise