AB2377

Anti-Mast Cell Chymase antibody [CC1]

Name: Anti-Mast Cell Chymase antibody [CC1] (ab2377) | Abcam
Brand: Abcam Limited
SKU: AB2377
Price: 200 USD
Availability: InStock
Rating: 2 (3 reviews)

BOND RX™ Validated
Recombinant
What is this?

(3 Reviews)

(44 Publications)

Mouse Recombinant Monoclonal Mast Cell Chymase antibody. Suitable for IHC-P and reacts with Human samples. Cited in 44 publications.

View Alternative Names

CYH, CYM, CMA1, Chymase, Alpha-chymase, Mast cell protease I

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377)

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Mast Cell Chymase with ab2377 at 1/20000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377)

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma labeling Mast Cell Chymase with ab2377 at 1/20000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Mast Cell Chymase with ab2377 at 1/20000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Mast Cell Chymase CD3 with ab2377 at 1/20000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CC1

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20000", "IHCP-species-notes": "<p></p>" } } }

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Product details

This product has switched from a hybridoma to recombinant production method on 1st November 2024.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.

See full target information CMA1

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Publications (44)

Recent publications for all applications. Explore the full list and refine your search

Current issues in molecular biology 47: PubMed41020874

2025

Phenotypic and Quantitative Characterization of Mast Cells in Cutaneous Melanoma: Correlation with Staging Metrics.

Applications

Unspecified application

Species

Unspecified reactive species

Grigory Demyashkin,Dmitrii Atiakshin,Kirill Silakov,Vladimir Shchekin,Maxim Bobrov,Olga Abramova,Matvey Vadyukhin,Tatyana Borovaya,Ekaterina Blinova,Petr Shegay,Andrei Kaprin

Medical sciences (Basel, Switzerland) 13: PubMed40981193

2025

Mast Cell Association with the Microenvironment of a Phosphaturic Mesenchymal Tumour Secreting Fibroblast Growth Factor 23.

Applications

Unspecified application

Species

Unspecified reactive species

Andrey Kostin,Alexei Lyundup,Alexander Alekhnovich,Aleksandra Prikhodko,Olga Patsap,Sofia Gronskaia,Zhanna Belaya,Olga Lesnyak,Galina Melnichenko,Natalia Mokrysheva,Igor Buchwalow,Markus Tiemann,Dmitrii Atiakshin

International journal of molecular sciences 25: PubMed39273142

2024

The Contribution of Mast Cells to the Regulation of Elastic Fiber Tensometry in the Skin Dermis of Children with Marfan Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitrii Atiakshin,Ekaterina Nikolaeva,Alla Semyachkina,Andrey Kostin,Artem Volodkin,Sergey Morozov,Michael Ignatyuk,Liudmila Mikhaleva,Grigory Demyashkin,Daniel Elieh-Ali-Komi,Igor Buchwalow,Markus Tiemann

Cell reports. Medicine 5:101709 PubMed39226895

2024

Decoding the pathogenesis of spermatogenic failure in cryptorchidism through single-cell transcriptomic profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Wang,Qiang Liu,Ziyan Zhuang,Jianxing Cheng,Wenxiu Zhang,Qiaoling Jiang,Yifei Guo,Ran Li,Xiaojian Lu,Lina Cui,Jiaming Weng,Yanlin Tang,Jingwei Yue,Songzhan Gao,Kai Hong,Jie Qiao,Hui Jiang,Jingtao Guo,Zhe Zhang

Frontiers in aging neuroscience 16:1376756 PubMed38979113

2024

Peripheral mast cells derive the effects of acupuncture in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ju-Young Oh,Sun-Jeong Bae,Jeong-Yeon Ji,Tae-Yeon Hwang,Suhwan Ji,Ji-Yeun Park,Seung-Nam Kim,Yeonhee Ryu,Min-Ho Nam,Hi-Joon Park

Discover oncology 15:208 PubMed38834833

2024

Characteristics of mast cell infiltration in lung adenocarcinoma and its impact on prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zijian Qiu,Guanchao Pang,Xia Xu,Jun Lin,Pingli Wang

ERJ open research 10: PubMed38500797

2024

Differential expression of mast cells in the small airways and alveolar septa of current smokers and patients with small airway disease and COPD.

Applications

Unspecified application

Species

Unspecified reactive species

Mathew Suji Eapen,Wenying Lu,Surajit Dey,Collin Chia,Ashutosh Hardikar,Md Imtaiyaz Hassan,Prem Bhattarai,Archana Vijay Gaikwad,Shatarupa Das,Philip M Hansbro,Gurpreet Kaur Singhera,Tillie-Louise Hackett,Sukhwinder Singh Sohal

Cell & bioscience 13:217 PubMed38031173

2023

Single-cell analysis unveils activation of mast cells in colorectal cancer microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyu Xie,Liaoran Niu,Gaozan Zheng,Kunli Du,Songchen Dai,Ruikai Li,Hanjun Dan,Lili Duan,Hongze Wu,Guangming Ren,Xinyu Dou,Fan Feng,Jian Zhang,Jianyong Zheng

ERJ open research 9: PubMed37077555

2023

Endothelial-to-mesenchymal transition: a precursor to pulmonary arterial remodelling in patients with idiopathic pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Archana Vijay Gaikwad,Wenying Lu,Surajit Dey,Prem Bhattarai,Greg Haug,Josie Larby,Collin Chia,Jade Jaffar,Glen Westall,Gurpreet Kaur Singhera,Tillie-Louise Hackett,Mathew Suji Eapen,Sukhwinder Singh Sohal

Frontiers in cardiovascular medicine 10:1132786 PubMed37265571

2023

Impact of early pericardial fluid chymase activation after cardiac surgery.

Applications

Unspecified application

Species

Unspecified reactive species

Brittany Butts,Lee A Goeddel,Jingyi Zheng,Betty Pat,Pamela Powell,James Mobley,Sarfaraz Ahmad,Chad Steele,David McGiffin,James E Davies,James F George,Spencer J Melby,Carlos M Ferrario,Louis J Dell'Italia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mast Cell Chymase antibody [CC1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (44)

Product promise