Mouse Recombinant Monoclonal Mast Cell Chymase antibody. Suitable for IHC-P and reacts with Human samples. Cited in 35 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-chymase-antibody-cc1-ab2377--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441546.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-chymase-antibody-cc1-ab2377--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441547.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-chymase-antibody-cc1-ab2377--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441548.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-chymase-antibody-cc1-ab2377--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441549.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (AB2377)")
Publications
Filter by
- Cell & bioscience 13:2172023Single-cell analysis unveils activation of mast cells in colorectal cancer microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenyu Xie,Liaoran Niu,Gaozan Zheng,Kunli Du,Songchen Dai,Ruikai Li,Hanjun Dan,Lili Duan,Hongze Wu,Guangming Ren,Xinyu Dou,Fan Feng,Jian Zhang,Jianyong ZhengPubMed 38031173
- ERJ open research 9:2023Endothelial-to-mesenchymal transition: a precursor to pulmonary arterial remodelling in patients with idiopathic pulmonary fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArchana Vijay Gaikwad,Wenying Lu,Surajit Dey,Prem Bhattarai,Greg Haug,Josie Larby,Collin Chia,Jade Jaffar,Glen Westall,Gurpreet Kaur Singhera,Tillie-Louise Hackett,Mathew Suji Eapen,Sukhwinder Singh SohalPubMed 37077555
- Frontiers in cardiovascular medicine 10:11327862023Impact of early pericardial fluid chymase activation after cardiac surgery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrittany Butts,Lee A Goeddel,Jingyi Zheng,Betty Pat,Pamela Powell,James Mobley,Sarfaraz Ahmad,Chad Steele,David McGiffin,James E Davies,James F George,Spencer J Melby,Carlos M Ferrario,Louis J Dell'ItaliaPubMed 37265571
- Cell biology international 47:1136-11462023Bisphenol A disruption promotes mammary tumor microenvironment via phenotypic cell polarization and inflammatory response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThalles F R Ruiz,Simone J Colleta,Diego D Dos Santos,Nayara F C Castro,Ágata S Cabral,Marilia F Calmon,Paula Rahal,Cristiane D Gil,Ana Paula Girol,Patricia S L Vilamaior,Ellen C R Leonel,Sebastião R TabogaPubMed 36906806
- Histochemistry and cell biology 159:353-3612023Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDmitri Atiakshin,Andrey Kostin,Igor Buchwalow,Denis Morozow,Vera Samoilova,Markus TiemannPubMed 36598563
- Acta neuropathologica communications 9:1362021The pathogenic role of c-Kit+ mast cells in the spinal motor neuron-vascular niche in ALS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariángeles Kovacs,Catalina Alamón,Cecilia Maciel,Valentina Varela,Sofía Ibarburu,Lucas Tarragó,Peter H King,Ying Si,Yuri Kwon,Olivier Hermine,Luis Barbeito,Emiliano TriasPubMed 34389060
- Histochemistry and cell biology 155:561-5802021Protease profile of normal and neoplastic mast cells in the human bone marrow with special emphasis on systemic mastocytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDmitri Atiakshin,Igor Buchwalow,Peter Horny,Markus TiemannPubMed 33492488
- International journal of oncology 56:1093-11002020Mast cell chymase promotes angiogenesis and lymphangiogenesis mediated by activation of melanoma inhibitory activity gene family members in oral squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiyako Kurihara-Shimomura et. al.PubMed 32319583
- JACC. Basic to translational science 5:109-1222020Reduced Left Atrial Emptying Fraction and Chymase Activation in Pathophysiology of Primary Mitral Regurgitation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrittany Butts,Mustafa I Ahmed,Navkaranbir S Bajaj,Pamela Cox Powell,Betty Pat,Silvio Litovsky,Himanshu Gupta,Steven G Lloyd,Thomas S Denney,Xiaoxia Zhang,Inmaculada Aban,Sakthivel Sadayappan,James W McNamara,Michael J Watson,Carlos M Ferrario,James F Collawn,Clifton Lewis,James E Davies,Louis J Dell'ItaliaPubMed 32140620
- Scientific reports 9:193752019Rhubarb-Evoke Mucus Secretion through Aggregation and Degranulation of Mast Cell in the Colon of Rat: In vivo and ex vivo studies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDi Wu et. al.PubMed 31852975
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20000 | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Alternative names
CYH, CYM, CMA1, Chymase, Alpha-chymase, Mast cell protease I
Recommended products
Mouse Recombinant Monoclonal Mast Cell Chymase antibody. Suitable for IHC-P and reacts with Human samples. Cited in 35 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- CC1
- Purification technique
- Affinity purification Protein A
- Light chain type
- unknown
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product has switched from a hybridoma to recombinant production method on 1st November 2024.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-chymase-antibody-cc1-ab2377--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441546.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Mast Cell Chymase CD3 with ab2377 at 1/20000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-chymase-antibody-cc1-ab2377--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441547.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Mast Cell Chymase with ab2377 at 1/20000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-chymase-antibody-cc1-ab2377--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441548.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma labeling Mast Cell Chymase with ab2377 at 1/20000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-chymase-antibody-cc1-ab2377--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441549.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Mast Cell Chymase with ab2377 at 1/20000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com