Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal

• mIHC

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Multiplex immunohistochemistry - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (AB186417)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human tonsil tissue labeling Allergin 1 with ab310327 at 1/100 Panel A : merged staining of anti-Mast Cell Chymase (green; Opal™520) and anti-Allergin-1 (red; Opal™570) on human tonsil. Panel B : anti-Allergin-1 stained on various immune cells. Panel C : anti-Mast Cell Chymase stained on mast cells. The section was incubated in two rounds of staining : in the order of ab186417 and ab310327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Counterstained with DAPI. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (AB186417)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human appendix tissue labeling Allergin 1 with ab310327 at 1/100 Panel A : merged staining of anti-Mast Cell Chymase (green; Opal™520) and anti-Allergin-1 (red; Opal™570) on human appendix. Panel B : anti-Allergin-1 stained on various immune cells. Panel C : anti-Mast Cell Chymase stained on mast cells. The section was incubated in two rounds of staining : in the order of ab186417 and ab310327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Counterstained with DAPI. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

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Western blot - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (AB186417)

All lanes:

Western blot - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417) at 1/10000 dilution

All lanes:

Human small intestine tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (AB186417)

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling Mast Cell Chymase with ab186417 at 1/500 dilution. Secondary staining with HRP Polymer for Rabbit IgG and counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.