Rabbit Recombinant Monoclonal TRYB1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (AB134931), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr8475-ab134931--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23208.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (AB134931)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (AB134931), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr8475-ab134931--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74271.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (AB134931)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (AB134931), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr8475-ab134931--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129950.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (AB134931)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (AB134931), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr8475-ab134931--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46541.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (AB134931)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (AB134931), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr8475-ab134931--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131532.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (AB134931)")
Publications
Filter by
- Journal of cellular physiology 234:18283-182962019Prolonged elevated levels of c-kit+ progenitor cells after a myocardial infarction by beta 2 adrenergic receptor priming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmanda Finan et. al.PubMed 30912139
- BMC cancer 18:2302018Stellate cells and mesenchymal stem cells in benign mammary stroma are associated with risk factors for breast cancer - an observational study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBjörn Logi Isfoss et. al.PubMed 29486751
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes For antigen retrieval: heat up to 98 °C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed:18854315).
Alternative names
TPS1, TPS2, TPSB1, TPSAB1, Tryptase alpha/beta-1, Tryptase-1, Tryptase I, Tryptase alpha-1
Recommended products
Rabbit Recombinant Monoclonal TRYB1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR8475
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (ab134931), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr8475-ab134931--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23208.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (ab134931)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (ab134931)
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human colon tissue, labelling Mast Cell Tryptase using ab134931 at a 1/500 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (ab134931), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr8475-ab134931--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74271.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (ab134931)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (ab134931)
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human tonsil tissue, labelling Mast Cell Tryptase using ab134931 at a 1/500 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (ab134931), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr8475-ab134931--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129950.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (ab134931)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (ab134931)
Immunohistochemical analysis using ab134931 showing positive staining in Normal stomach tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (ab134931), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr8475-ab134931--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46541.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (ab134931)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (ab134931)
Immunohistochemical analysis using ab134931 showing positive staining in Breast carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (ab134931), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr8475-ab134931--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131532.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (ab134931)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] (ab134931)
Immunohistochemical analysis using ab134931 showing positive staining in Colonic adenocarcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com