Rabbit Recombinant Monoclonal TRYB1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 14 publications.
Images
![Western blot - Anti-Mast Cell Tryptase antibody [EPR9522] (AB151757), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr9522-ab151757--western-blot-img71115.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Mast Cell Tryptase antibody [EPR9522] (AB151757)")
![Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] (AB151757), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr9522-ab151757--immunoprecipitation-img3314.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] (AB151757)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] (AB151757), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr9522-ab151757--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134742.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] (AB151757)")
Publications
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- International journal of molecular sciences 24:2023Amyloid Beta Peptides Lead to Mast Cell Activation in a Novel 3D Hydrogel Model.Applications:ICC/IFReactive species:Unspecified reactive speciesJingshu Liu,Sihan Liu,Li Zeng,Irene TsilioniPubMed 37569378
- Cancer research communications 3:896-9072023EGFR Inhibition by Cetuximab Modulates Hypoxia and IFN Response Genes in Head and Neck Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRitu Chaudhary,Robbert J C Slebos,Leenil C Noel,Feifei Song,Maria I Poole,Dirk S Hoening,Juan C Hernandez-Prera,Jose R Conejo-Garcia,Jose A Guevara-Patino,Xuefeng Wang,Mengyu Xie,Aik Choon Tan,Christine H ChungPubMed 37377902
- Gut microbes 14:21050952022Gut bacteria interact directly with colonic mast cells in a humanized mouse model of IBS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiko Shimbori,Giada De Palma,Lauren Baerg,Jun Lu,Elena F Verdu,David E Reed,Stephen Vanner,Stephen M Collins,Premysl BercikPubMed 35905313
- Cells 10:2021Expression of CD38 in Mast Cells: Cytological and Histotopographic Features.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDmitri Atiakshin,Vera Samoilova,Igor Buchwalow,Markus TiemannPubMed 34685490
- Journal of cachexia, sarcopenia and muscle 12:1079-10972021Activated mast cells in skeletal muscle can be a potential mediator for cancer-associated cachexia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesD Brooke Widner,Chun Liu,Qingxia Zhao,Sarah Sharp,Matthew R Eber,Sun H Park,D Clark Files,Yusuke ShiozawaPubMed 34008339
- Histochemistry and cell biology 155:561-5802021Protease profile of normal and neoplastic mast cells in the human bone marrow with special emphasis on systemic mastocytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDmitri Atiakshin,Igor Buchwalow,Peter Horny,Markus TiemannPubMed 33492488
- Veterinary pathology 58:181-2042020Development of Mast Cell and Eosinophil Hyperplasia and HLH/MAS-Like Disease in NSG-SGM3 Mice Receiving Human CD34+ Hematopoietic Stem Cells or Patient-Derived Leukemia Xenografts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura J Janke,Denise M Imai,Heather Tillman,Rosalinda Doty,Mark J Hoenerhoff,Jiajie J Xu,Zachary T Freeman,Portia Allen,Natalie Wall Fowlkes,Ilaria Iacobucci,Kirsten Dickerson,Charles G Mullighan,Peter Vogel,Jerold E RehgPubMed 33208054
- Science advances 5:eaax58512019MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure.Applications:Mass CytometryReactive species:Unspecified reactive speciesLeeat Keren et. al.PubMed 31633026
- Journal of molecular neuroscience : MN 69:235-2452019Inhibition of Mast Cell Degranulation Relieves Visceral Hypersensitivity Induced by Pancreatic Carcinoma in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDawei Yu et. al.PubMed 31201657
- The Journal of allergy and clinical immunology 143:1027-1037.e72018Genetic diversity between mouse strains allows identification of the CC027/GeniUnc strain as an orally reactive model of peanut allergy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKelly Orgel,Johanna M Smeekens,Ping Ye,Lauren Fotsch,Rishu Guo,Darla R Miller,Fernando Pardo-Manuel de Villena,A Wesley Burks,Martin T Ferris,Michael D KulisPubMed 30342892
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
1 product for Alternative Version
Target data
Function
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed:18854315).
Targets
TPSB2
Alternative names
Tryptase alpha/beta-1, Tryptase-1, Tryptase I, Tryptase alpha-1, TPSB1, TPS1, TPS2, TPSAB1
Recommended products
Rabbit Recombinant Monoclonal TRYB1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 14 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR9522
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr9522-ab151757--western-blot-img71115.jpg "Western blot - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)")
Western blot - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)
All lanes: Western blot - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757) at 1/1000 dilution
Lane 1: Human skin lysate at 10 µg
Lane 2: Human colon cancer lysate at 10 µg
Lane 3: Human tonsil lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 22 kDa, 31 kDa
![Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr9522-ab151757--immunoprecipitation-img3314.jpg "Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)")
Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)
Purified ab151757 at 1/50 dilution (2μg) immunoprecipitating Mast Cell Tryptase in Human skin lysate.
Lane 1 (input): Human skin lysate 10μg
Lane 2 (+): ab151757 + Human skin lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab151757 in Human skin lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 30 kDaAll lanes: Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)
Predicted band size: 31 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr9522-ab151757--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134742.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)
Immunohistochemical analysis of paraffin embedded Human tissue labeling Mast Cell Tryptase with ab151757 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
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