Rabbit Recombinant Monoclonal TRYB1 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Images
![Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (AB216451), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr9522-low-endotoxin-azide-free-ab216451--immunoprecipitation-img3314.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (AB216451)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (AB216451), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr9522-low-endotoxin-azide-free-ab216451--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134742.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (AB216451)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
2 products for Alternative Version
Target data
Function
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed:18854315).
Alternative names
Tryptase alpha/beta-1, Tryptase-1, Tryptase I, Tryptase alpha-1, TPSB1, TPS1, TPS2, TPSAB1
Recommended products
Rabbit Recombinant Monoclonal TRYB1 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR9522
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab216451 is the carrier-free version of Anti-Mast Cell Tryptase antibody [EPR9522] ab151757.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (ab216451), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr9522-low-endotoxin-azide-free-ab216451--immunoprecipitation-img3314.jpg "Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (ab216451)")
Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (ab216451)
This data was developed using Anti-Mast Cell Tryptase antibody [EPR9522] ab151757, the same antibody clone in a different buffer formulation.
Purified Anti-Mast Cell Tryptase antibody [EPR9522] ab151757 at 1/50 dilution (2μg) immunoprecipitating Mast Cell Tryptase in Human skin lysate.
Lane 1 (input): Human skin lysate 10μg
Lane 2 (+): Anti-Mast Cell Tryptase antibody [EPR9522] ab151757 + Human skin lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Mast Cell Tryptase antibody [EPR9522] ab151757 in Human skin lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 30 kDaAll lanes: Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] (Anti-Mast Cell Tryptase antibody [EPR9522] ab151757)
Predicted band size: 31 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (ab216451), expandable thumbnail](https://content.abcam.com/products/images/mast-cell-tryptase-antibody-epr9522-low-endotoxin-azide-free-ab216451--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134742.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (ab216451)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (ab216451)
Immunohistochemical analysis of paraffin embedded Human tissue labeling Mast Cell Tryptase with Anti-Mast Cell Tryptase antibody [EPR9522] ab151757 at a 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mast Cell Tryptase antibody [EPR9522] ab151757).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
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