AB174687

Anti-MAT1A antibody

Name: Anti-MAT1A antibody (ab174687) | Abcam
Brand: Abcam Limited
SKU: AB174687
Price: 605 USD
Availability: InStock

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(3 Publications )

Rabbit Polyclonal MAT1A antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MAT1A aa 100-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

AMS1, MATA1, MAT1A, S-adenosylmethionine synthase isoform type-1, AdoMet synthase 1, Methionine adenosyltransferase 1, Methionine adenosyltransferase I/III, MAT 1, MAT-I/III

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT1A antibody (AB174687)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT1A antibody (AB174687)

Immunohistochemical analysis of paraffin embedded formalin fixed Human lung tissue labeling MAT1A using ab174687 at 5 μg/ml.

Supplier Data

Western blot - Anti-MAT1A antibody (AB174687)

All lanes:

Western blot - Anti-MAT1A antibody (ab174687) at 1/100 dilution

All lanes:

MDA-MB435 cell line lysates at 35 µg

Predicted band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MAT1A aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q00266

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAT1A also known as Methionine Adenosyltransferase 1A is an enzyme with a molecular mass of approximately 41 kDa. This enzyme is integral in the catalysis of the formation of S-adenosylmethionine (SAM) from methionine and ATP a fundamental reaction in cellular metabolism. MAT1A is expressed predominantly in the liver reflecting its significant role in hepatic function.

Biological function summary

This enzyme participates in critical cellular processes involving methylation. It is part of the methionine adenosyltransferase complex which is essential for supplying S-adenosylmethionine to multiple methylating reactions in the cell. These reactions include DNA methylation an important process in gene expression regulation and histone methylation influencing chromatin structure.

Pathways

MAT1A plays a role in the methionine cycle and transsulfuration pathway. These pathways are important for amino acid metabolism and synthesis detoxification and antioxidant production. The enzyme collaborates with MAT2A another isoenzyme which plays a role outside the liver in SAM synthesis thereby coordinating methylation processes across tissues.

Mutations or dysregulation of MAT1A can relate to liver diseases such as cirrhosis and liver cancer. These conditions can arise due to impaired SAM production that affects methylation and consequently gene expression and cellular maintenance. MAT1A interactions with MAT2A are important as imbalances in SAM levels can influence cancer progression in hepatic and non-hepatic tissues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme : formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

See full target information MAT1A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 20: PubMed31600961

2019

Methionine Adenosyltransferase 1a (MAT1A) Enhances Cell Survival During Chemotherapy Treatment and is Associated with Drug Resistance in Bladder Cancer PDX Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly A Martin,Nicholas R Hum,Aimy Sebastian,Wei He,Salma Siddiqui,Paramita M Ghosh,Chong-Xian Pan,Ralph de Vere White,Gabriela G Loots

Life sciences 234:116771 PubMed31421084

2019

MAT1 facilitates the lung metastasis of osteosarcoma through upregulation of AKT1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Wang,Jiangdong Ni,Deye Song,Muliang Ding,Jun Huang,Wenzhao Li,Guangxu He

EBioMedicine 42:458-469 PubMed30926424

2019

TGF-β1/p65/MAT2A pathway regulates liver fibrogenesis via intracellular SAM.

Applications

Unspecified application

Species

Unspecified reactive species

Kuifeng Wang,Shanhua Fang,Qian Liu,Jing Gao,Xiaoning Wang,Hongwen Zhu,Zhenyun Zhu,Feihong Ji,Jiasheng Wu,Yueming Ma,Lihong Hu,Xu Shen,Daming Gao,Jiansheng Zhu,Ping Liu,Hu Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAT1A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT1A antibody (AB174687)

Western blot - Anti-MAT1A antibody (AB174687)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise