AB229608

Anti-MAT1A + MAT2A antibody

Name: Anti-MAT1A + MAT2A antibody (ab229608) | Abcam
Brand: Abcam Limited
SKU: AB229608
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal MAT1A antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate - Human, Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MAT1A.

View Alternative Names

AMS1, MATA1, MAT1A, S-adenosylmethionine synthase isoform type-1, AdoMet synthase 1, Methionine adenosyltransferase 1, Methionine adenosyltransferase I/III, MAT 1, MAT-I/III, AMS2, MATA2, MAT2A, S-adenosylmethionine synthase isoform type-2, AdoMet synthase 2, Methionine adenosyltransferase 2, Methionine adenosyltransferase II, MAT 2, MAT-II

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT1A + MAT2A antibody (AB229608)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT1A + MAT2A antibody (AB229608)

Paraffin-embedded mouse liver tissue stained for MAT1A + MAT2A with ab229608 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT1A + MAT2A antibody (AB229608)

Paraffin-embedded rat liver tissue stained for MAT1A + MAT2A with ab229608 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-MAT1A + MAT2A antibody (AB229608)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-MAT1A + MAT2A antibody (ab229608) at 1/1000 dilution

All lanes:

Mouse liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 44 kDa

false

Supplier Data

Western blot - Anti-MAT1A + MAT2A antibody (AB229608)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-MAT1A + MAT2A antibody (ab229608) at 1/1000 dilution

All lanes:

Rat liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 44 kDa

false

Supplier Data

Western blot - Anti-MAT1A + MAT2A antibody (AB229608)

Samples were separated by 10% SDS-PGAE. The signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-MAT1A + MAT2A antibody (ab229608) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

MAT1A transfected (+) 293T whole cell extracts at 30 µg

Lane 3:

MAT2A transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-mouse IgG antibody

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MAT1A. The exact immunogen used to generate this antibody is proprietary information.

Q00266

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAT1A also known as Methionine Adenosyltransferase 1A is an enzyme with a molecular mass of approximately 41 kDa. This enzyme is integral in the catalysis of the formation of S-adenosylmethionine (SAM) from methionine and ATP a fundamental reaction in cellular metabolism. MAT1A is expressed predominantly in the liver reflecting its significant role in hepatic function.

Biological function summary

This enzyme participates in critical cellular processes involving methylation. It is part of the methionine adenosyltransferase complex which is essential for supplying S-adenosylmethionine to multiple methylating reactions in the cell. These reactions include DNA methylation an important process in gene expression regulation and histone methylation influencing chromatin structure.

Pathways

MAT1A plays a role in the methionine cycle and transsulfuration pathway. These pathways are important for amino acid metabolism and synthesis detoxification and antioxidant production. The enzyme collaborates with MAT2A another isoenzyme which plays a role outside the liver in SAM synthesis thereby coordinating methylation processes across tissues.

Mutations or dysregulation of MAT1A can relate to liver diseases such as cirrhosis and liver cancer. These conditions can arise due to impaired SAM production that affects methylation and consequently gene expression and cellular maintenance. MAT1A interactions with MAT2A are important as imbalances in SAM levels can influence cancer progression in hepatic and non-hepatic tissues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme : formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

See full target information MAT1A

Additional targets

MAT2A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 17:110527 PubMed40977645

2025

Transcription factor 3 enhances hepatocellular carcinoma metastasis by upregulating matrix metalloproteinase-11.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Peng Tian,Tian-Hao Wu,Guang-Jun Zhang,Sheng-Jie Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAT1A + MAT2A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT1A + MAT2A antibody (AB229608)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT1A + MAT2A antibody (AB229608)

Western blot - Anti-MAT1A + MAT2A antibody (AB229608)

Western blot - Anti-MAT1A + MAT2A antibody (AB229608)

Western blot - Anti-MAT1A + MAT2A antibody (AB229608)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise