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Rabbit Recombinant Monoclonal MAT2A antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples.

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Images

Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (AB192846), expandable thumbnail
  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (AB192846), expandable thumbnail
  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (AB192846), expandable thumbnail
  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (AB192846), expandable thumbnail
  • Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR19048] (AB192846), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested
Recombinant full length protein - Human
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

Additional Targets

MAT1A

Alternative names

AMS2, MATA2, MAT2A, S-adenosylmethionine synthase isoform type-2, AdoMet synthase 2, Methionine adenosyltransferase 2, Methionine adenosyltransferase II, MAT 2, MAT-II

Recommended products

Rabbit Recombinant Monoclonal MAT2A antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19048
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MAT1A and MAT2A are enzymes also known as methionine adenosyltransferase 1A and 2A which play a critical role in the synthesis of S-adenosylmethionine (SAMe). MAT1A has a molecular mass of about 42 kDa while MAT2A varies slightly due to post-translational modifications. These enzymes are mainly expressed in the liver and extrahepatic tissues respectively. MAT1A predominantly functions in the liver where it serves an essential part in maintaining normal liver metabolism.

Biological function summary

Methionine adenosyltransferase 1A and 2A function to catalyze the transfer of an adenosyl group from ATP to methionine forming SAMe. This reaction is part of a critical metabolic process acting as a source of methyl groups for methylation reactions. MAT1A commonly works as part of a homotetrameric complex while MAT2A can form both dimers and tetramers. This flexibility in complex formation helps regulate the production of SAMe based on cellular demands linking it to vital biomolecules synthesis.

Pathways

Methionine salvage pathways and polyamine biosynthesis include the significant involvement of MAT1A and MAT2A. These enzymes provide the necessary SAMe for the transmethylation and transsulfuration pathways connecting to other proteins such as GNMT and SAH hydrolase. The enzymes' activity influences the levels of important intermediates impacting these pathways and resulting metabolic outputs.

Associated diseases and disorders

Abnormal expression or dysfunction of methionine adenosyltransferase 1A and 2A associates with liver cirrhosis and hepatocellular carcinoma. Alterations in the enzymes’ activity can disrupt normal SAMe levels affecting methylation reactions and subsequent gene expression patterns. Key proteins like cyclin-dependent kinase (CDK) also interact with these enzymes further linking them to cancer progression and outcomes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846), expandable thumbnail

    Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

    Exposure times: Lane 1: 1 second; Lane 2: 2 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human MAT1A recombinant protein contains aa1-395 with a His-Tag®. This recombinant protein was made in-house.

    All lanes: Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/20000 dilution

    Lane 1: Human MAT1A recombinant protein at 0.01 µg

    Lane 2: Human MAT2A recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa

    Observed band size: 44 kDa, 75 kDa

  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846), expandable thumbnail

    Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Lane 1: Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution

    Lanes 2 - 3: Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/5000 dilution

    Lane 1: Human fetal liver tissue lysate at 10 µg

    Lane 2: Mouse liver tissue lysate at 10 µg

    Lane 3: Rat liver tissue lysate at 10 µg

    Secondary

    Lane 1: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Lanes 2 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa

    Observed band size: 44 kDa

    Exposure time: 1s

  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846), expandable thumbnail

    Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Lanes 1 - 3: Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/10000 dilution

    Lanes 4 - 6: Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution

    Lane 1: Ramos (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

    Lane 2: U937 (human histiocytic lymphoma cell line) whole cell lysate at 10 µg

    Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

    Lane 4: Human fetal heart tissue lysate at 10 µg

    Lane 5: Human fetal kidney tissue lysate at 10 µg

    Lane 6: Human fetal spleen tissue lysate at 10 µg

    Secondary

    Lanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lanes 4 - 6: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa

    Observed band size: 44 kDa

    Exposure time: 3s

  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846), expandable thumbnail

    Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

    Exposure times: Lane 1-2: 5 seconds; Lane 3-6: 3 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution

    Lane 1: Mouse kidney tissue lysate at 10 µg

    Lane 2: Mouse spleen tissue lysate at 10 µg

    Lane 3: Rat brain tissue lysate at 10 µg

    Lane 4: Rat kidney tissue lysate at 10 µg

    Lane 5: C6 (rat glial tumor cell line) whole cell lysate at 10 µg

    Lane 6: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa

    Observed band size: 44 kDa

  • Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846), expandable thumbnail

    Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

    MAT2A was immunoprecipitated from 0.35 mg of Ramos (human Burkitt's lymphoma cell line) whole cell lysate with ab192846 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab192846 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Ramos whole cell lysate 10 μg (Input).

    Lane 2: ab192846 IP in Ramos whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab192846 in Ramos whole cell lysate.

    Exposure time: 1 second.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

    Observed band size: 44 kDa

  • Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846), expandable thumbnail

    Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 18695670 and PMID: 22879628).

    All lanes: Western blot - Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/20000 dilution

    Lane 1: Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

    Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) treated with 500 ng/ml Lipopolysaccharides for 4 hours, whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa

    Observed band size: 44 kDa

    Exposure time: 5s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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