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AB129176

Anti-MAT1A + MAT2A antibody [EPR7938]

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(7 Publications)

Rabbit Recombinant Monoclonal MAT1A antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 7 publications.

View Alternative Names

AMS1, MATA1, MAT1A, S-adenosylmethionine synthase isoform type-1, AdoMet synthase 1, Methionine adenosyltransferase 1, Methionine adenosyltransferase I/III, MAT 1, MAT-I/III

5 Images
Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR7938] (AB129176)
  • IP

Unknown

Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR7938] (AB129176)

ab129176 (Purified) at 1 : 40 dilution (2 µg) immunoprecipitating MAT1A in 293T whole cell lysate.
Lane 1 (input) : 293T (Human embryonic kidney epithelial cell) whole cell lysate 10 µg
Lane 2 (+) : ab129176 & 293T whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab129176 in 293T whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129176)

All lanes:

Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR7938] (ab129176)

Predicted band size: 44 kDa

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Western blot - Anti-MAT1A + MAT2A antibody [EPR7938] (AB129176)
  • WB

Lab

Western blot - Anti-MAT1A + MAT2A antibody [EPR7938] (AB129176)

All lanes:

Western blot - Anti-MAT1A + MAT2A antibody [EPR7938] (ab129176) at 1/10000 dilution

Lane 1:

Human fetal liver lysates at 20 µg

Lane 2:

Mouse liver lysates at 20 µg

Lane 3:

Rat liver lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa

false

Western blot - Anti-MAT1A + MAT2A antibody [EPR7938] (AB129176)
  • WB

Unknown

Western blot - Anti-MAT1A + MAT2A antibody [EPR7938] (AB129176)

All lanes:

Western blot - Anti-MAT1A + MAT2A antibody [EPR7938] (ab129176) at 1/1000 dilution

Lane 1:

Fetal liver lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa

false

Western blot - Anti-MAT1A + MAT2A antibody [EPR7938] (AB129176)
  • WB

Lab

Western blot - Anti-MAT1A + MAT2A antibody [EPR7938] (AB129176)

Blocking and diluting buffer and concentration : 5% NFDM /TBST.

Exposure time :
Lane 1 & 2 : 3 seconds
Lane 3 & 4 : 40 seconds

Lanes 1 - 2:

Western blot - Anti-MAT1A + MAT2A antibody [EPR7938] (ab129176) at 1/1000 dilution

Lanes 3 - 4:

Western blot - Anti-GST antibody [EPR4236] (<a href='/en-us/products/primary-antibodies/gst-antibody-epr4236-ab111947'>ab111947</a>)

Lanes 1 and 3:

GST-tagged full length MAT1A (Human) recombinant protein at 10 ng

Lanes 2 and 4:

GST-tagged full length MAT2A (Human) recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa

false

OI-RD Scanning - Anti-MAT1A + MAT2A antibody [EPR7938] (AB129176)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-MAT1A + MAT2A antibody [EPR7938] (AB129176)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-MAT1A + MAT2A antibody [EPR7938] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7938

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAT1A also known as Methionine Adenosyltransferase 1A is an enzyme with a molecular mass of approximately 41 kDa. This enzyme is integral in the catalysis of the formation of S-adenosylmethionine (SAM) from methionine and ATP a fundamental reaction in cellular metabolism. MAT1A is expressed predominantly in the liver reflecting its significant role in hepatic function.
Biological function summary

This enzyme participates in critical cellular processes involving methylation. It is part of the methionine adenosyltransferase complex which is essential for supplying S-adenosylmethionine to multiple methylating reactions in the cell. These reactions include DNA methylation an important process in gene expression regulation and histone methylation influencing chromatin structure.

Pathways

MAT1A plays a role in the methionine cycle and transsulfuration pathway. These pathways are important for amino acid metabolism and synthesis detoxification and antioxidant production. The enzyme collaborates with MAT2A another isoenzyme which plays a role outside the liver in SAM synthesis thereby coordinating methylation processes across tissues.

Mutations or dysregulation of MAT1A can relate to liver diseases such as cirrhosis and liver cancer. These conditions can arise due to impaired SAM production that affects methylation and consequently gene expression and cellular maintenance. MAT1A interactions with MAT2A are important as imbalances in SAM levels can influence cancer progression in hepatic and non-hepatic tissues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme : formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.
See full target information MAT1A

Additional targets

MAT2A
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107783 PubMed39303913

2024

Downregulation of the mA reader YTHDC2 upregulates exosome content in lung adenocarcinoma via inhibiting IFIT and OAS family members.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Yin,Lifang Ma,Xiaoting Tian,Qi Sun,Congcong Zhang,Yikun Wang,Yayou Miao,Xiangfei Xue,Yongjie Wang,Jiayi Wang,Xiao Zhang,Xumin Hou

Cell metabolism 35:2183-2199.e7 PubMed38006878

2023

PRMT1 orchestrates with SAMTOR to govern mTORC1 methionine sensing via Arg-methylation of NPRL2.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Jiang,Jing Liu,Shaohui He,Wei Xu,Runzhi Huang,Weijuan Pan,Xiaolong Li,Xiaoming Dai,Jianping Guo,Tao Zhang,Hiroyuki Inuzuka,Ping Wang,John M Asara,Jianru Xiao,Wenyi Wei

Cell reports 37:109958 PubMed34758301

2021

The hepatic compensatory response to elevated systemic sulfide promotes diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Roderick N Carter,Matthew T G Gibbins,Martin E Barrios-Llerena,Stephen E Wilkie,Peter L Freddolino,Marouane Libiad,Victor Vitvitsky,Barry Emerson,Thierry Le Bihan,Madara Brice,Huizhong Su,Scott G Denham,Natalie Z M Homer,Clare Mc Fadden,Anne Tailleux,Nourdine Faresse,Thierry Sulpice,Francois Briand,Tom Gillingwater,Kyo Han Ahn,Subhankar Singha,Claire McMaster,Richard C Hartley,Bart Staels,Gillian A Gray,Andrew J Finch,Colin Selman,Ruma Banerjee,Nicholas M Morton

Nature communications 11:3978 PubMed32770044

2020

HNF4α regulates sulfur amino acid metabolism and confers sensitivity to methionine restriction in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Xu,Yuanyuan Li,Xia Gao,Kai Kang,Jason G Williams,Lingfeng Tong,Juan Liu,Ming Ji,Leesa J Deterding,Xuemei Tong,Jason W Locasale,Leping Li,Igor Shats,Xiaoling Li

Cancer cell international 20:316 PubMed32694938

2020

Cyclin H predicts the poor prognosis and promotes the proliferation of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Peng,Yansong Yang,Li Ji,Panpan Yang,Xiaoqing Yang,Yuquan Zhang

Cell metabolism 32:100-116.e4 PubMed32413334

2020

Untangling Determinants of Enhanced Health and Lifespan through a Multi-omics Approach in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Aon,Michel Bernier,Sarah J Mitchell,Clara Di Germanio,Julie A Mattison,Margaux R Ehrlich,Ricki J Colman,Rozalyn M Anderson,Rafael de Cabo

Cell systems 6:579-592.e4 PubMed29778837

2018

Defining Host Responses during Systemic Bacterial Infection through Construction of a Murine Organ Proteome Atlas.

Applications

Unspecified application

Species

Unspecified reactive species

John D Lapek,Robert H Mills,Jacob M Wozniak,Anaamika Campeau,Ronnie H Fang,Xiaoli Wei,Kirsten van de Groep,Araceli Perez-Lopez,Nina M van Sorge,Manuela Raffatellu,Rob Knight,Liangfang Zhang,David J Gonzalez
View all publications
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