AB248332

Anti-MAT1A + MAT2A antibody [EPR7938] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MAT1A antibody. Carrier free. Suitable for IP, WB and reacts with Human, Recombinant full length protein - Human, Mouse, Rat samples.

View Alternative Names

AMS1, MATA1, MAT1A, S-adenosylmethionine synthase isoform type-1, AdoMet synthase 1, Methionine adenosyltransferase 1, Methionine adenosyltransferase I/III, MAT 1, MAT-I/III

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Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR7938] - BSA and Azide free (AB248332)

ab129176 (Purified) at 1 : 40 dilution (2 μg) immunoprecipitating MAT1A in 293T whole cell lysate.
Lane 1 (input) : 293T (Human embryonic kidney epithelial cell) whole cell lysate 10 μg
Lane 2 (+) : ab129176 & 293T whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab129176 in 293T whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129176)

All lanes:

Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR7938] (<a href='/en-us/products/primary-antibodies/mat1a-mat2a-antibody-epr7938-ab129176'>ab129176</a>)

Predicted band size: 44 kDa

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Lab

Western blot - Anti-MAT1A + MAT2A antibody [EPR7938] - BSA and Azide free (AB248332)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129176).

Blocking and diluting buffer and concentration : 5% NFDM /TBST.

Exposure time :
Lane 1 & 2 : 3 seconds
Lane 3 & 4 : 40 seconds

Lanes 1 - 2:

Western blot - Anti-MAT1A + MAT2A antibody [EPR7938] (<a href='/en-us/products/primary-antibodies/mat1a-mat2a-antibody-epr7938-ab129176'>ab129176</a>) at 1/1000 dilution

Lanes 3 - 4:

Western blot - Anti-GST antibody [EPR4236] (<a href='/en-us/products/primary-antibodies/gst-antibody-epr4236-ab111947'>ab111947</a>)

Lanes 1 and 3:

GST-tagged full length MAT1A (Human) recombinant protein at 10 ng

Lanes 2 and 4:

GST-tagged full length MAT2A (Human) recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7938

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab248332 is the carrier-free version of ab129176.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAT1A also known as Methionine Adenosyltransferase 1A is an enzyme with a molecular mass of approximately 41 kDa. This enzyme is integral in the catalysis of the formation of S-adenosylmethionine (SAM) from methionine and ATP a fundamental reaction in cellular metabolism. MAT1A is expressed predominantly in the liver reflecting its significant role in hepatic function.

Biological function summary

This enzyme participates in critical cellular processes involving methylation. It is part of the methionine adenosyltransferase complex which is essential for supplying S-adenosylmethionine to multiple methylating reactions in the cell. These reactions include DNA methylation an important process in gene expression regulation and histone methylation influencing chromatin structure.

Pathways

MAT1A plays a role in the methionine cycle and transsulfuration pathway. These pathways are important for amino acid metabolism and synthesis detoxification and antioxidant production. The enzyme collaborates with MAT2A another isoenzyme which plays a role outside the liver in SAM synthesis thereby coordinating methylation processes across tissues.

Mutations or dysregulation of MAT1A can relate to liver diseases such as cirrhosis and liver cancer. These conditions can arise due to impaired SAM production that affects methylation and consequently gene expression and cellular maintenance. MAT1A interactions with MAT2A are important as imbalances in SAM levels can influence cancer progression in hepatic and non-hepatic tissues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme : formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

See full target information MAT1A

Additional targets

MAT2A

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAT1A + MAT2A antibody [EPR7938] - BSA and Azide free

Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR7938] - BSA and Azide free (AB248332)

Western blot - Anti-MAT1A + MAT2A antibody [EPR7938] - BSA and Azide free (AB248332)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise