Rabbit Polyclonal MAT2A antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAT2A aa 1-300.
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- Cell death & disease 14:5912023METTL16 controls Kaposi's sarcoma-associated herpesvirus replication by regulating S-adenosylmethionine cycle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinquan Zhang,Wen Meng,Jian Feng,Xinghong Gao,Chao Qin,Pinghui Feng,Yufei Huang,Shou-Jiang GaoPubMed 37673880
- Cell & bioscience 12:1832022mTORC1-c-Myc pathway rewires methionine metabolism for HCC progression through suppressing SIRT4 mediated ADP ribosylation of MAT2A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Zhao,Huizhao Su,Xiaomeng Liu,Hongquan Wang,Yukuan Feng,Yan Wang,Haiqiang Chen,Luo Dai,Shihui Lai,Siqi Xu,Chong Li,Jihui Hao,Bo TangPubMed 36371321
- Cell journal 24:204-2112022Inhibition of MAT2A-Related Methionine Metabolism Enhances The Efficacy of Cisplatin on Cisplatin-Resistant Cells in Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoya Zhao,Lude Wang,Haiping Lin,Jing Wang,Jianfei Fu,Dan Zhu,Wenxia XuPubMed 35674024
- Nature communications 11:13442020Impact of the gut microbiota on the mA epitranscriptome of mouse cecum and liver.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSabrina Jabs et. al.PubMed 32165618
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MAT2A aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P31153
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cow | Predicted |
| Monkey | Predicted |
| Pig | Predicted |
| Xenopus laevis | Predicted |
| Xenopus tropicalis | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig, Xenopus laevis, Monkey, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
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Target data
Function
Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.
Alternative names
AMS2, MATA2, MAT2A, S-adenosylmethionine synthase isoform type-2, AdoMet synthase 2, Methionine adenosyltransferase 2, Methionine adenosyltransferase II, MAT 2, MAT-II
Recommended products
Rabbit Polyclonal MAT2A antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAT2A aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MAT2A aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P31153
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MAT2A or methionine adenosyltransferase 2A is an enzyme that plays an important role in the methionine cycle by catalyzing the conversion of methionine to S-adenosylmethionine (SAM). It has a molecular mass of approximately 43 kDa. MAT2A is widely expressed in various tissues with high levels typically found in the liver and kidney. This protein has alternative names including MAT-II and AdoMet synthetase. Its activity is critical for maintaining cellular methylation processes that affect a vast array of cellular functions.
- Biological function summary
Methionine adenosyltransferase 2A is essential for the synthesis of SAM which is a primary methyl donor in cellular methylation reactions. MAT2A often works as part of a larger protein complex that regulates cellular growth and division. It is not an isolated component but participates in complexes that ensure proper methyl donation throughout various biological activities. The enzyme's role in methylation impacts DNA RNA and protein modifications which are essential for gene expression regulation and cellular response.
- Pathways
Methionine adenosyltransferase 2A is significant in the methionine metabolism pathway closely interlinking with the transsulfuration pathway. This pathway involvement connects MAT2A with key metabolic regulators like SAM and cystathionine beta-synthase ensuring balanced methionine homeostasis and redox status. MAT2A also interacts with proteins like MAT1A which performs similar functions in the liver showing interdependency within pathways that govern methylation and sulfur amino acid metabolism.
- Associated diseases and disorders
Dysregulation of the methionine adenosyltransferase 2A has been implicated in liver cancer and neurodegenerative diseases. Aberrant expression or mutations in MAT2A can lead to disrupted SAM synthesis which contributes to altered methylation patterns observed in hepatocellular carcinoma (HCC). Additionally changes in MAT2A expression are relevant to neurodegenerative conditions as it interacts indirectly with proteins such as MAT1A which is also involved in hepatic pathologies. Understanding MAT2A's role provides insights into potential therapeutic targets for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-MAT2A antibody (ab154343)
10% SDS PAGE
All lanes: Western blot - Anti-MAT2A antibody (ab154343) at 1/1000 dilution
All lanes: 293T whole cell lysate at 30 µg
Predicted band size: 44 kDa, 49 kDa, 54 kDa
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