AB154343

Anti-MAT2A antibody

Name: Anti-MAT2A antibody (ab154343) | Abcam
Brand: Abcam Limited
SKU: AB154343

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(7 Publications)

Rabbit Polyclonal MAT2A antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAT2A aa 1-300.

View Alternative Names

AMS2, MATA2, MAT2A, S-adenosylmethionine synthase isoform type-2, AdoMet synthase 2, Methionine adenosyltransferase 2, Methionine adenosyltransferase II, MAT 2, MAT-II

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT2A antibody (AB154343)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT2A antibody (AB154343)

Immunohistochemical analysis of paraffin embedded mouse esophagus tissue stained for MAT1A + MAT2A protein at cytoplasm and nucleus with ab154343 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT2A antibody (AB154343)

Immunohistochemical analysis of paraffin embedded rat colon tissue stained for MAT1A + MAT2A protein at cytoplasm and nucleus with ab154343 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT2A antibody (AB154343)

Immunohistochemical analysis of paraffin embedded rat colon tissue stained for MAT1A + MAT2A protein at cytoplasm and nucleus with ab154343 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-MAT2A antibody (AB154343)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-MAT2A antibody (ab154343) at 1/2000 dilution

All lanes:

293T whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 44 kDa

false

Supplier Data

Western blot - Anti-MAT2A antibody (AB154343)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-MAT2A antibody (ab154343) at 1/2000 dilution

Lane 1:

Daudi whole cell extracts at 30 µg

Lane 2:

MCF-7 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MAT2A aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P31153

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAT2A or methionine adenosyltransferase 2A is an enzyme that plays an important role in the methionine cycle by catalyzing the conversion of methionine to S-adenosylmethionine (SAM). It has a molecular mass of approximately 43 kDa. MAT2A is widely expressed in various tissues with high levels typically found in the liver and kidney. This protein has alternative names including MAT-II and AdoMet synthetase. Its activity is critical for maintaining cellular methylation processes that affect a vast array of cellular functions.

Biological function summary

Methionine adenosyltransferase 2A is essential for the synthesis of SAM which is a primary methyl donor in cellular methylation reactions. MAT2A often works as part of a larger protein complex that regulates cellular growth and division. It is not an isolated component but participates in complexes that ensure proper methyl donation throughout various biological activities. The enzyme's role in methylation impacts DNA RNA and protein modifications which are essential for gene expression regulation and cellular response.

Pathways

Methionine adenosyltransferase 2A is significant in the methionine metabolism pathway closely interlinking with the transsulfuration pathway. This pathway involvement connects MAT2A with key metabolic regulators like SAM and cystathionine beta-synthase ensuring balanced methionine homeostasis and redox status. MAT2A also interacts with proteins like MAT1A which performs similar functions in the liver showing interdependency within pathways that govern methylation and sulfur amino acid metabolism.

Dysregulation of the methionine adenosyltransferase 2A has been implicated in liver cancer and neurodegenerative diseases. Aberrant expression or mutations in MAT2A can lead to disrupted SAM synthesis which contributes to altered methylation patterns observed in hepatocellular carcinoma (HCC). Additionally changes in MAT2A expression are relevant to neurodegenerative conditions as it interacts indirectly with proteins such as MAT1A which is also involved in hepatic pathologies. Understanding MAT2A's role provides insights into potential therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme : formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

See full target information MAT2A

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:591 PubMed37673880

2023

METTL16 controls Kaposi's sarcoma-associated herpesvirus replication by regulating S-adenosylmethionine cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Xinquan Zhang,Wen Meng,Jian Feng,Xinghong Gao,Chao Qin,Pinghui Feng,Yufei Huang,Shou-Jiang Gao

Journal of bone oncology 41:100490 PubMed37457846

2023

MAT2A inhibits the ferroptosis in osteosarcoma progression regulated by miR-26b-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Shuchi Xia,Yun Liang,Yuqing Shen,Wuxue Zhong,Yiqun Ma

Cell & bioscience 12:183 PubMed36371321

2022

mTORC1-c-Myc pathway rewires methionine metabolism for HCC progression through suppressing SIRT4 mediated ADP ribosylation of MAT2A.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhao,Huizhao Su,Xiaomeng Liu,Hongquan Wang,Yukuan Feng,Yan Wang,Haiqiang Chen,Luo Dai,Shihui Lai,Siqi Xu,Chong Li,Jihui Hao,Bo Tang

Cell journal 24:204-211 PubMed35674024

2022

Inhibition of MAT2A-Related Methionine Metabolism Enhances The Efficacy of Cisplatin on Cisplatin-Resistant Cells in Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoya Zhao,Lude Wang,Haiping Lin,Jing Wang,Jianfei Fu,Dan Zhu,Wenxia Xu

Brain research bulletin 175:107-115 PubMed34284075

2021

MiR-26b-5p-modified hUB-MSCs derived exosomes attenuate early brain injury during subarachnoid hemorrhage via MAT2A-mediated the p38 MAPK/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zunwei Liu,Bo Wang,Qihang Guo

Nature communications 11:1344 PubMed32165618

2020

Impact of the gut microbiota on the mA epitranscriptome of mouse cecum and liver.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Jabs,Anne Biton,Christophe Bécavin,Marie-Anne Nahori,Amine Ghozlane,Alessandro Pagliuso,Giulia Spanò,Vincent Guérineau,David Touboul,Quentin Giai Gianetto,Thibault Chaze,Mariette Matondo,Marie-Agnès Dillies,Pascale Cossart

Scientific reports 9:19089 PubMed31836741

2019

MicroRNA-509-3p inhibits cellular migration, invasion, and proliferation, and sensitizes osteosarcoma to cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Sagar L Patil,Asha Palat,Yinghong Pan,Kimal Rajapakshe,Rachna Mirchandani,Maria Bondesson,Jason T Yustein,Cristian Coarfa,Preethi H Gunaratne

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MAT2A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT2A antibody (AB154343)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT2A antibody (AB154343)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT2A antibody (AB154343)

Western blot - Anti-MAT2A antibody (AB154343)

Western blot - Anti-MAT2A antibody (AB154343)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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