AB186129

Anti-MAT2A antibody - N-terminal

Name: Anti-MAT2A antibody - N-terminal (ab186129) | Abcam
Brand: Abcam Limited
SKU: AB186129
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal MAT2A antibody. N-terminal. Suitable for WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MAT2A aa 1-50.

View Alternative Names

AMS2, MATA2, MAT2A, S-adenosylmethionine synthase isoform type-2, AdoMet synthase 2, Methionine adenosyltransferase 2, Methionine adenosyltransferase II, MAT 2, MAT-II

1 Images

Supplier Data

Western blot - Anti-MAT2A antibody - N-terminal (AB186129)

All lanes:

Western blot - Anti-MAT2A antibody - N-terminal (ab186129) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 44 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human MAT2A aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P31153

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab186129 was affinity purified using an epitope specific to MAT2A immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAT2A or methionine adenosyltransferase 2A is an enzyme that plays an important role in the methionine cycle by catalyzing the conversion of methionine to S-adenosylmethionine (SAM). It has a molecular mass of approximately 43 kDa. MAT2A is widely expressed in various tissues with high levels typically found in the liver and kidney. This protein has alternative names including MAT-II and AdoMet synthetase. Its activity is critical for maintaining cellular methylation processes that affect a vast array of cellular functions.

Biological function summary

Methionine adenosyltransferase 2A is essential for the synthesis of SAM which is a primary methyl donor in cellular methylation reactions. MAT2A often works as part of a larger protein complex that regulates cellular growth and division. It is not an isolated component but participates in complexes that ensure proper methyl donation throughout various biological activities. The enzyme's role in methylation impacts DNA RNA and protein modifications which are essential for gene expression regulation and cellular response.

Pathways

Methionine adenosyltransferase 2A is significant in the methionine metabolism pathway closely interlinking with the transsulfuration pathway. This pathway involvement connects MAT2A with key metabolic regulators like SAM and cystathionine beta-synthase ensuring balanced methionine homeostasis and redox status. MAT2A also interacts with proteins like MAT1A which performs similar functions in the liver showing interdependency within pathways that govern methylation and sulfur amino acid metabolism.

Dysregulation of the methionine adenosyltransferase 2A has been implicated in liver cancer and neurodegenerative diseases. Aberrant expression or mutations in MAT2A can lead to disrupted SAM synthesis which contributes to altered methylation patterns observed in hepatocellular carcinoma (HCC). Additionally changes in MAT2A expression are relevant to neurodegenerative conditions as it interacts indirectly with proteins such as MAT1A which is also involved in hepatic pathologies. Understanding MAT2A's role provides insights into potential therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme : formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

See full target information MAT2A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:191 PubMed40263268

2025

HSP90 regulates dCK stability and inhibits ionizing radiation-induced ferroptosis in cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Wang,Huilin Ji,Tianpeng Yang,Yi Liu,Xiang He,Xinyue Jiang,Zipeng Lu,Liu Han,Xiaodong Liu,Shumei Ma

MedComm 5:e705 PubMed39309689

2024

SCR-7952, a highly selective MAT2A inhibitor, demonstrates synergistic antitumor activities in combination with the -adenosylmethionine-competitive or the methylthioadenosine-cooperative protein arginine methyltransferase 5 inhibitors in methylthioadenosine phosphorylase-deleted tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyong Yu,Yi Kuang,Liting Xue,Xuan Ma,Tingting Li,Linlin Yuan,Mengying Li,Grace Xue,Zhen Li,Feng Tang,Jianxing Tang,Jinwen Shan,Weijie Wang,Renhong Tang,Feng Zhou

Journal of immunology research 2021:5510869 PubMed34258296

2021

Circ_0044516 Regulates miR-136/MAT2A Pathway to Facilitate Lung Cancer Development.

Applications

Unspecified application

Species

Unspecified reactive species

Yue-Wei Chen,Qiu-Rong Du,Yu-Juan He,Wen-Shu Chen,Wen-Yang Jiang,Qi Gui,Cheng-Cheng Xu,Wei Wang,Hong-Yun Cheng

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MAT2A antibody - N-terminal

Western blot - Anti-MAT2A antibody - N-terminal (AB186129)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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