Anti-MAT2A + MAT1A antibody [EPR10496]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MAT2A + MAT1A antibody [EPR10496] (AB177484)

Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MAT2A+MAT1A with Purified ab177484 at 1 : 100 dilution (2.52 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MAT2A + MAT1A antibody [EPR10496] (AB177484)

Intracellular Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling MAT2A+MAT1A with Purified ab177484 at 1/30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MAT2A + MAT1A antibody [EPR10496] (AB177484)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MAT2A+MAT1A with Purified ab177484 at 1/30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• WB

Unknown

Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (AB177484)

MAT1A : 44kDa ; MAT2A : 46kDa

Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 6:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa,46 kDa

false

• WB

Lab

Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (AB177484)

MAT1A : Full-length recombinant protein (aa1-395) with His-Tag was made in-house
MAT2A : Full-length recombinant protein (aa1-364) with GST-Tag was from Abnova company

Blocking and diluting buffer and concentrations : 5%NFDM/TBST

Exposure time : Lane 1 : 1 second
Lane 2 : 3 seconds

All lanes:

Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484) at 1/10000 dilution

Lane 1:

Full-length Human MAT1A recombinant protein with His-Tag at 0.015 µg

Lane 2:

Full-length Human MAT2A recombinant protein with GST-Tag at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 44 kDa

false