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Rabbit Recombinant Monoclonal MAT1A antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 5 publications.

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Images

Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (AB177484), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MAT2A + MAT1A antibody [EPR10496] (AB177484), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MAT2A + MAT1A antibody [EPR10496] (AB177484), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MAT2A + MAT1A antibody [EPR10496] (AB177484), expandable thumbnail
  • Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (AB177484), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Recombinant full length protein - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

1/1000 - 1/5000

Notes

-

Species

Mouse

Dilution info

1/1000 - 1/5000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/5000

Notes

-

Species

Human

Dilution info

1/1000 - 1/5000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Target data

Function

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

Targets

S-adenosylmethionine synthase isoform type-2

Alternative names

Recommended products

Rabbit Recombinant Monoclonal MAT1A antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 5 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR10496

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MAT2A (Methionine Adenosyltransferase 2A) and MAT1A (Methionine Adenosyltransferase 1A) are enzymes that catalyze the formation of S-adenosylmethionine (SAM) from methionine and ATP. MAT2A also known as hepatic MAT has a mass of approximately 52 kDa while MAT1A also sometimes referred to as extrahepatic MAT typically weighs around 48 kDa. Both enzymes differ in their tissue expression: MAT1A is predominantly found in the liver whereas MAT2A expresses across various tissues such as the brain and intestines.

Biological function summary

MAT2A and MAT1A are key in the methionine cycle impacting gene regulation through methylation reactions. They facilitate the synthesis of SAM a universal methyl donor essential for transmethylation processes within cells. Specifically MAT1A forms a complex with glycine N-methyltransferase to regulate homocysteine and methylation homeostasis. Both enzymes perform a role in controlling the abundance of SAM which influences various cellular processes including nucleic acid protein and lipid metabolism.

Pathways

MAT2A and MAT1A participate actively in methionine and SAM metabolism. They integrate within the one-carbon metabolism pathway which is critical for methylation processes affecting gene expression and epigenetic regulation. MAT2A interacts with proteins like MAT2B which modulates the activity of MAT2A impacting the balance between SAM and S-adenosylhomocysteine. This pathway interaction is important for maintaining methylation balance and cellular proliferation.

Associated diseases and disorders

MAT2A and MAT1A relate to liver diseases and cancer reflecting their roles in SAM metabolism. Abnormal function or expression of these enzymes affects SAM levels linking to liver cirrhosis and hepatocellular carcinoma. MAT1A mutations can impair methionine metabolism exacerbating liver conditions. Similarly altered MAT2A expression associates with certain cancers where it modulates cellular methylation states and growth. Proteins like SAM might get affected creating an imbalance that contributes to the pathology of liver disease and oncogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484), expandable thumbnail

    Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

    MAT1A: 44kDa ; MAT2A: 46kDa

    Blocking Buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

    Lane 4: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 5: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 6: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa

    Observed band size: 44 kDa, 46 kDa

    This data was developed using ab177484, the same antibody clone in a different buffer formulation.

    MAT1A: 44kDa ; MAT2A: 46kDa

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MAT2A+MAT1A with Purified ab177484 at 1:100 dilution (2.52 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

    Intracellular Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling MAT2A+MAT1A with Purified ab177484 at 1/30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Flow Cytometry (Intracellular) - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MAT2A+MAT1A with Purified ab177484 at 1/30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484), expandable thumbnail

    Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484)

    MAT1A: Full-length recombinant protein (aa1-395) with His-Tag was made in-house
    MAT2A: Full-length recombinant protein (aa1-364) with GST-Tag was from Abnova company

    Blocking and diluting buffer and concentrations: 5%NFDM/TBST

    Exposure time: Lane 1: 1 second
    Lane 2: 3 seconds

    All lanes: Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484) at 1/10000 dilution

    Lane 1: Full-length Human MAT1A recombinant protein with His-Tag at 0.015 µg

    Lane 2: Full-length Human MAT2A recombinant protein with GST-Tag at 0.015 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa

    This data was developed using ab177484, the same antibody clone in a different buffer formulation.

    MAT1A: Full-length recombinant protein (aa1-395) with His-Tag was made in-house.

    MAT2A: Full-length recombinant protein (aa1-364) with GST-Tag was from Abnova company.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second. Lane 2: 3 seconds.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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