Rabbit Recombinant Monoclonal MAT1A antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 5 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
ICC/IF | WB | Flow Cyt (Intra) | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
Rat | Expected | Tested | Expected |
Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info 1/1000 - 1/5000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/5000 | Notes - |
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.
S-adenosylmethionine synthase isoform type-2
AMS1, MATA1, AMS1, MAT1A, MATA1, S-adenosylmethionine synthase isoform type-1, AdoMet synthase 1, Methionine adenosyltransferase 1, Methionine adenosyltransferase I/III, MAT 1, MAT-I/III
Rabbit Recombinant Monoclonal MAT1A antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 5 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR10496
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
MAT2A (Methionine Adenosyltransferase 2A) and MAT1A (Methionine Adenosyltransferase 1A) are enzymes that catalyze the formation of S-adenosylmethionine (SAM) from methionine and ATP. MAT2A also known as hepatic MAT has a mass of approximately 52 kDa while MAT1A also sometimes referred to as extrahepatic MAT typically weighs around 48 kDa. Both enzymes differ in their tissue expression: MAT1A is predominantly found in the liver whereas MAT2A expresses across various tissues such as the brain and intestines.
MAT2A and MAT1A are key in the methionine cycle impacting gene regulation through methylation reactions. They facilitate the synthesis of SAM a universal methyl donor essential for transmethylation processes within cells. Specifically MAT1A forms a complex with glycine N-methyltransferase to regulate homocysteine and methylation homeostasis. Both enzymes perform a role in controlling the abundance of SAM which influences various cellular processes including nucleic acid protein and lipid metabolism.
MAT2A and MAT1A participate actively in methionine and SAM metabolism. They integrate within the one-carbon metabolism pathway which is critical for methylation processes affecting gene expression and epigenetic regulation. MAT2A interacts with proteins like MAT2B which modulates the activity of MAT2A impacting the balance between SAM and S-adenosylhomocysteine. This pathway interaction is important for maintaining methylation balance and cellular proliferation.
MAT2A and MAT1A relate to liver diseases and cancer reflecting their roles in SAM metabolism. Abnormal function or expression of these enzymes affects SAM levels linking to liver cirrhosis and hepatocellular carcinoma. MAT1A mutations can impair methionine metabolism exacerbating liver conditions. Similarly altered MAT2A expression associates with certain cancers where it modulates cellular methylation states and growth. Proteins like SAM might get affected creating an imbalance that contributes to the pathology of liver disease and oncogenesis.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
MAT1A: 44kDa ; MAT2A: 46kDa
Blocking Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg
Lane 4: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 5: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 6: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa, 46 kDa
This data was developed using ab177484, the same antibody clone in a different buffer formulation.
MAT1A: 44kDa ; MAT2A: 46kDa
Blocking Buffer and concentration: 5% NFDM/TBST
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MAT2A+MAT1A with Purified ab177484 at 1:100 dilution (2.52 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Intracellular Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling MAT2A+MAT1A with Purified ab177484 at 1/30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MAT2A+MAT1A with Purified ab177484 at 1/30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
MAT1A: Full-length recombinant protein (aa1-395) with His-Tag was made in-house
MAT2A: Full-length recombinant protein (aa1-364) with GST-Tag was from Abnova company
Blocking and diluting buffer and concentrations: 5%NFDM/TBST
Exposure time: Lane 1: 1 second
Lane 2: 3 seconds
All lanes: Western blot - Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484) at 1/10000 dilution
Lane 1: Full-length Human MAT1A recombinant protein with His-Tag at 0.015 µg
Lane 2: Full-length Human MAT2A recombinant protein with GST-Tag at 0.015 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
This data was developed using ab177484, the same antibody clone in a different buffer formulation.
MAT1A: Full-length recombinant protein (aa1-395) with His-Tag was made in-house.
MAT2A: Full-length recombinant protein (aa1-364) with GST-Tag was from Abnova company.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 second. Lane 2: 3 seconds.
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