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AB139132

Anti-MAT2B/TGR antibody

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(1 Publication)

Goat Polyclonal MAT2B/TGR antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TGR, MSTP045, Nbla02999, UNQ2435/PRO4995, MAT2B, Methionine adenosyltransferase 2 subunit beta, Methionine adenosyltransferase II beta, Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase, MAT II beta

1 Images
Western blot - Anti-MAT2B/TGR antibody (AB139132)
  • WB

Unknown

Western blot - Anti-MAT2B/TGR antibody (AB139132)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-MAT2B/TGR antibody (ab139132) at 0.3 µg/mL

All lanes:

Human thymus lysate in RIPA buffer at 35 µg

Predicted band size: 38 kDa

Observed band size: 37 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab139132 is expected to recognize reported Isoform 1 (NP_037415.1) only.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab139132 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAT2B also known as TGR is a protein that functions as a regulatory subunit of the enzyme methionine adenosyltransferase II. This enzyme synthesizes S-adenosylmethionine (SAM) essential for methylation reactions in varied cellular processes. MAT2B has a molecular mass of approximately 35 kDa and can modulate the activity of the catalytic subunit of the methionine adenosyltransferase complex. The protein is expressed in multiple tissues with abundant levels in liver and fetal tissues. It is important for regulating the levels of SAM linking dietary methionine to cellular methylation capacity.
Biological function summary

The MAT2B protein helps maintain cellular methylation status by adjusting SAM synthesis. It is a part of the methionine adenosyltransferase II complex which consists of different subunits contributing to its enzymatic activity. The regulatory role of MAT2B ensures precise control over the methionine cycle impacting DNA methylation patterns and gene regulation. By modulating SAM production MAT2B influences cellular growth differentiation and apoptosis through its interactions with other methylation-related proteins.

Pathways

The MAT2B protein plays a significant role in methionine metabolism and the transmethylation pathway. These pathways are central to transferring methyl groups from SAM to a range of substrates including DNA proteins and lipids. MAT2B is related to MAT2A the catalytic subunit that generates SAM. Together they coordinate to balance cellular metabolic needs with the synthesis and utilization of methyl groups securing the methylation-dependent regulation of diverse cellular processes.

MAT2B associates with liver diseases and cancer. Its regulatory function in SAM synthesis ties it to disruptions in methylation capacity observed in these conditions. Reduced MAT2B activity can lead to imbalanced SAM levels affecting the methylation landscape and contributing to altered gene expression involved in tumorigenesis. In liver diseases MAT2B interacts with MAT1A a protein facilitating methylation balance in mature liver tissue. This interaction highlights MAT2B's significant impact on maintaining liver health and its potential role in pathogenesis when dysregulated.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of S-adenosylmethionine synthetase 2, an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates MAT2A catalytic activity by changing its kinetic properties, increasing its affinity for L-methionine (PubMed : 10644686, PubMed : 23189196, PubMed : 25075345). Can bind NADP (in vitro) (PubMed : 23189196, PubMed : 23425511).
See full target information MAT2B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:7779 PubMed31123282

2019

BRAF inhibition sensitizes melanoma cells to α-amanitin via decreased RNA polymerase II assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas Frischknecht,Christian Britschgi,Patricia Galliker,Yann Christinat,Anton Vichalkovski,Matthias Gstaiger,Werner J Kovacs,Wilhelm Krek
View all publications
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Product promise

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