JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB92295

Anti-MATE-1 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Goat Polyclonal MATE-1 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SLC47A1 aa 500-550.

View Alternative Names

MATE1, SLC47A1, Multidrug and toxin extrusion protein 1, MATE-1, hMATE-1, Solute carrier family 47 member 1

2 Images
Immunocytochemistry - Anti-MATE-1 antibody (AB92295)
  • ICC

Supplier Data

Immunocytochemistry - Anti-MATE-1 antibody (AB92295)

Immunocytochemistry analysis of paraformaldehyde fixed HepG2 cells labeling MATE-1. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2 μg/mL). The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2 μg/mL).

Western blot - Anti-MATE-1 antibody (AB92295)
  • WB

Supplier Data

Western blot - Anti-MATE-1 antibody (AB92295)

Primary incubation 1 hour at room temperature. Detected by chemiluminescence.

All lanes:

Western blot - Anti-MATE-1 antibody (ab92295) at 0.1 µg/mL

Lane 1:

Human liver lysate (in RIPA buffer) at 35 µg

Lane 2:

Human liver lysate (in RIPA buffer) at 35 µg with immunizing peptide

Predicted band size: 61 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC

applications

Immunogen

Synthetic Peptide within Human SLC47A1 aa 500-550. The exact immunogen used to generate this antibody is proprietary information.

Q96FL8

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MATE-1 also known as SLC47A1 is a transporter protein that plays an important role in the efflux of organic cations. It has a mass of approximately 56 kDa and is localized on the apical membrane of renal proximal tubule cells and canalicular membrane of hepatocytes. MATE-1 mediates the expulsion of its substrates into the urine and bile using an exchange mechanism driven by the electrochemical gradient of protons across the membrane. This protein is linked with the protein family of multidrug and toxin extrusion transporters which have similar functional properties.
Biological function summary

MATE-1 contributes to the clearance of various endogenous and exogenous compounds from the body. It acts as part of a larger transporter network and works synergistically with other transporters such as OCT2 (organic cation transporter 2) to facilitate drug elimination. The protein is significant in the pharmacokinetics of many clinically relevant drugs including anti-mate compounds and antimicrobials. The coordinated activity of related transporters ensures precise regulation of drug concentration within systemic circulation.

Pathways

MATE-1 is integral to the organic cation transport pathway interacting with primary and secondary transporters including OCT1 and OCT2 to regulate cellular uptake and discharge of cationic drugs. Additionally MATE-1 is part of the detoxification pathway where it partners with enzymes like CYP450 to enhance the metabolism and excretion of toxicants from the body. Coordination within these pathways ensures effective clearance to maintain cellular integrity and function.

Alterations in MATE-1 expression or function associate with renal and hepatic disorders. For instance variations in MATE-1 can influence the disposition of drugs and toxins leading to nephrotoxicity. Furthermore disruptions in MATE-1 activity have connections with disorders like hyperuricemia where impaired excretion leads to increased serum urate levels. The close interaction of MATE-1 with urate transporters like GLUT9 further highlights its relevance in disease mechanisms.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Multidrug efflux pump that functions as a H(+)/organic cation antiporter (PubMed : 16330770, PubMed : 17509534). Plays a physiological role in the excretion of cationic compounds including endogenous metabolites, drugs, toxins through the kidney and liver, into urine and bile respectively (PubMed : 16330770, PubMed : 17495125, PubMed : 17509534, PubMed : 17582384, PubMed : 18305230, PubMed : 19158817, PubMed : 21128598, PubMed : 24961373). Mediates the efflux of endogenous compounds such as creatinine, vitamin B1/thiamine, agmatine and estrone-3-sulfate (PubMed : 16330770, PubMed : 17495125, PubMed : 17509534, PubMed : 17582384, PubMed : 18305230, PubMed : 19158817, PubMed : 21128598, PubMed : 24961373). May also contribute to regulate the transport of cationic compounds in testis across the blood-testis-barrier (Probable).
See full target information SLC47A1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110760 PubMed39286490

2024

Efficient proximal tubule-on-chip model from hiPSC-derived kidney organoids for functional analysis of renal transporters.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Ma,Ramin Banan Sadeghian,Ryosuke Negoro,Kazuya Fujimoto,Toshikazu Araoka,Naoki Ishiguro,Minoru Takasato,Ryuji Yokokawa

Proteomes 11: PubMed37092456

2023

Shotgun Proteomics of Co-Cultured Leukemic and Bone Marrow Stromal Cells from Different Species as a Preliminary Approach to Detect Intercellular Protein Transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Abraham Josué Nevárez-Ramírez,Ana Laura Guzmán-Ortiz,Pedro Cortes-Reynosa,Eduardo Perez-Salazar,Gustavo Alberto Jaimes-Ortega,Ricardo Valle-Rios,Álvaro Marín-Hernández,José S Rodríguez-Zavala,Eliel Ruiz-May,José Luis Castrejón-Flores,Héctor Quezada
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB224440

Anti-MATE-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATE-1 antibody (AB224440)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB135924

Anti-SLC22A6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC22A6 antibody (AB135924)

  • 4
  • 1
Primary Antibodies

AB247055

Anti-OAT3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OAT3 antibody (AB247055)

  • 4
  • 1
Primary Antibodies

AB312838

Anti-OATP1B1 antibody [EPR27070-49]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OATP1B1 antibody [EPR27070-49] (AB312838)

  • 0
  • 0
Primary Antibodies

AB230629

Anti-SLC22A2 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC22A2 antibody - C-terminal (AB230629)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com