AB85824

Anti-MATH2/NEUROD6 antibody

Name: Anti-MATH2/NEUROD6 antibody. Rabbit polyclonal (ab85824) | Abcam
Brand: Abcam Limited
SKU: AB85824
Price: 475 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(7 Publications)

Rabbit Polyclonal MATH2/NEUROD6 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

ATOH2, BHLHA2, My051, NEUROD6, Neurogenic differentiation factor 6, NeuroD6, Class A basic helix-loop-helix protein 2, Protein atonal homolog 2, bHLHa2

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-MATH2/NEUROD6 antibody (AB85824)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MATH2/NEUROD6 antibody (AB85824)

ab85824 staining MATH2/NEUROD6 in U-87 MG cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with the antibody ab85824 at 5μg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 μM for 1hour at room temperature.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Replenishment batches of ab85824 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MATH2 also known as NEUROD6 is important for the development and differentiation of neurons. It acts as a transcription factor regulating gene expression. With a molecular weight of approximately 39 kDa MATH2/NEUROD6 is expressed in the central nervous system particularly in the brain. This protein belongs to the basic helix-loop-helix (bHLH) family which are known for binding to specific DNA sequences to modulate transcription.

Biological function summary

MATH2/NEUROD6 plays a critical role in the nervous system. It influences neuronal survival outgrowth and synaptogenesis. Although not part of a larger protein complex it functions closely with other bHLH proteins to promote neurogenesis. Its similarity to NEUROD1 suggests overlap in their biological roles especially in the regulation of development in the nervous system.

Pathways

MATH2/NEUROD6 is key in the neurogenesis pathway facilitating the formation and specialization of neurons. It works alongside proteins such as MAPK which regulate various cellular activities including proliferation differentiation and response to extracellular stimuli. MATH2/NEUROD6 also interacts within the signaling pathways that affect neuronal differentiation aligning with other bHLH proteins for synaptic plasticity.

MATH2/NEUROD6 associates with neurodevelopmental disorders. Abnormal expression levels are linked to diseases such as Alzheimer's and Parkinson's due to their role in neuronal survival and plasticity. The protein's connection with NEUROD1 and its involvement in maintaining proper brain function underline its importance in research on neurological diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Activates E box-dependent transcription in collaboration with TCF3/E47. May be a trans-acting factor involved in the development and maintenance of the mammalian nervous system. Transactivates the promoter of its own gene (By similarity).

See full target information NEUROD6

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 10:917243 PubMed35959491

2022

mice with hirschsprung disease are missing Gad2-expressing enteric neurons in the ganglionated small intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Sukhada Bhave,Richard A Guyer,Nicole Picard,Meredith Omer,Ryo Hotta,Allan M Goldstein

Cerebral cortex (New York, N.Y. : 1991) 30:4689-4707 PubMed32249896

2020

Developmental Regulation of Basket Interneuron Synapses and Behavior through NCAM in Mouse Prefrontal Cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Chelsea S Sullivan,Vishwa Mohan,Paul B Manis,Sheryl S Moy,Young Truong,Bryce W Duncan,Patricia F Maness

iScience 13:452-477 PubMed30827871

2019

Alzheimer Aβ Assemblies Accumulate in Excitatory Neurons upon Proteasome Inhibition and Kill Nearby NAKα3 Neurons by Secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Hitomi Komura,Shota Kakio,Tomoya Sasahara,Yoshie Arai,Naomi Takino,Michio Sato,Kaori Satomura,Takayuki Ohnishi,Yo-Ichi Nabeshima,Shin-Ichi Muramatsu,Isao Kii,Minako Hoshi

Scientific reports 8:6143 PubMed29670169

2018

Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Chelsea S Sullivan,Ingo Gotthard,Elliott V Wyatt,Srihita Bongu,Vishwa Mohan,Richard J Weinberg,Patricia F Maness

Acta neuropathologica 135:855-875 PubMed29502200

2018

Differential α-synuclein expression contributes to selective vulnerability of hippocampal neuron subpopulations to fibril-induced toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Esteban Luna,Samantha C Decker,Dawn M Riddle,Anna Caputo,Bin Zhang,Tracy Cole,Carrie Caswell,Sharon X Xie,Virginia M Y Lee,Kelvin C Luk

The Journal of neuroscience : the official journal of the Society for Neuroscience 36:9454-71 PubMed27605619

2016

Pten Regulates Retinal Amacrine Cell Number by Modulating Akt, Tgfβ, and Erk Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Nobuhiko Tachibana,Robert Cantrup,Rajiv Dixit,Yacine Touahri,Gaurav Kaushik,Dawn Zinyk,Narsis Daftarian,Jeff Biernaskie,Sarah McFarlane,Carol Schuurmans

Proceedings of the National Academy of Sciences of 108:3288-93 PubMed21300888

2011

In vivo Atoh1 targetome reveals how a proneural transcription factor regulates cerebellar development.

Applications

ICC/IF

Species

Mouse

Tiemo J Klisch,Yuanxin Xi,Adriano Flora,Liguo Wang,Wei Li,Huda Y Zoghbi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MATH2/NEUROD6 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MATH2/NEUROD6 antibody (AB85824)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise