Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)

Rabbit Recombinant Monoclonal MATH2/NEUROD6 antibody. Suitable for IHC-P, WB, IP, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Transfected cell line - Mouse, Transfected cell lysate - Mouse samples.

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Images

Immunoprecipitation - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (AB315812), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	IP	ICC/IF	Flow Cyt (Intra)
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested	Tested
Rat	Tested	Tested	Expected	Tested	Expected
Transfected cell line - Mouse	Tested	Not recommended	Not recommended	Not recommended	Not recommended
Transfected cell lysate - Mouse	Not recommended	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Transfected cell line - Mouse	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Transfected cell lysate - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Transfected cell lysate - Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Transfected cell line - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Transfected cell line - Mouse, Transfected cell lysate - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -
Species Rat	Dilution info 1/50	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Transfected cell line - Mouse, Transfected cell lysate - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Transfected cell line - Mouse, Transfected cell lysate - Mouse	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information Neurod6

Function

Activates E box-dependent transcription in collaboration with TCF3/E47. May be a trans-acting factor involved in the development and maintenance of the mammalian nervous system. Transactivates the promoter of its own gene.

Alternative names

Ath2, Atoh2, Nex1, Neurod6, Neurogenic differentiation factor 6, NeuroD6, Helix-loop-helix protein mATH-2, Protein NEX-1, Protein atonal homolog 2, mATH2

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28660-23
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein MATH2 also known as NEUROD6 is important for the development and differentiation of neurons. It acts as a transcription factor regulating gene expression. With a molecular weight of approximately 39 kDa MATH2/NEUROD6 is expressed in the central nervous system particularly in the brain. This protein belongs to the basic helix-loop-helix (bHLH) family which are known for binding to specific DNA sequences to modulate transcription.

Biological function summary

MATH2/NEUROD6 plays a critical role in the nervous system. It influences neuronal survival outgrowth and synaptogenesis. Although not part of a larger protein complex it functions closely with other bHLH proteins to promote neurogenesis. Its similarity to NEUROD1 suggests overlap in their biological roles especially in the regulation of development in the nervous system.

Pathways

MATH2/NEUROD6 is key in the neurogenesis pathway facilitating the formation and specialization of neurons. It works alongside proteins such as MAPK which regulate various cellular activities including proliferation differentiation and response to extracellular stimuli. MATH2/NEUROD6 also interacts within the signaling pathways that affect neuronal differentiation aligning with other bHLH proteins for synaptic plasticity.

Associated diseases and disorders

MATH2/NEUROD6 associates with neurodevelopmental disorders. Abnormal expression levels are linked to diseases such as Alzheimer's and Parkinson's due to their role in neuronal survival and plasticity. The protein's connection with NEUROD1 and its involvement in maintaining proper brain function underline its importance in research on neurological diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

Immunoprecipitation - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)
MATH2/NEUROD6 was immunoprecipitated from 0.35 mg Mouse P5 cerebral cortex tissue lysate with ab315812 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315812 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse P5 cerebral cortex tissue lysate

Lane 2: ab315812 IP in Mouse P5 cerebral cortex tissue lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab315812 in mouse P5 cerebral cortex tissue lysate
All lanes: Immunoprecipitation - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812) at 1/30 dilution
All lanes: Mouse P5 cerebral cortex tissue lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: 293T cells transfected with a mouse NEUROD6 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 48 kDa, 36 kDa
Exposure time: 37s
Immunocytochemistry/ Immunofluorescence - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling MATH2/NEUROD6 with ab315812 at 1/50 (10.66 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing nuclear staining in rat primary neuron.

Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling MATH2/NEUROD6 with ab315812 at 1/50 (10.66 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing nuclear staining in mouse primary neurons.

Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse NeuroD6 expression vector containing a Myc-His tag. (B) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling MATH2/NEUROD6 with ab315812 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on HEK-293T transfected with a Myc-His-tagged mouse NeuroD6 construct (image A). No staining on HEK-293T transfected with empty plasmid (image B). The section was incubated with ab315812 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)
Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling MATH2/NEUROD6 with ab315812 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat lung. The section was incubated with ab315812 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling MATH2/NEUROD6 with ab315812 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat cardiac muscle. The section was incubated with ab315812 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling MATH2/NEUROD6 with ab315812 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse lung (PMID: 7545978). The section was incubated with ab315812 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling MATH2/NEUROD6 with ab315812 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse cardiac muscle (PMID: 7545978). The section was incubated with ab315812 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MATH2/NEUROD6 with ab315812 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebrum. The section was incubated with ab315812 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling MATH2/NEUROD6 with ab315812 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum (PMID: 7545978). The section was incubated with ab315812 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)
Negative control: heart, lung (PMID: 7545978, 7744035)
The identity of the lower MW band at approximately 25 kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812) at 1/1000 dilution
Lane 1: Mouse P5 cerebral cortex tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Mouse heart tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Mouse lung tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Rat P5 hippocampus tissue lysate at 20 µg with 5% NFDM/TBST
Lane 5: Rat heart tissue lysate at 20 µg with 5% NFDM/TBST
Lane 6: Rat lung tissue lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 48 kDa, 36 kDa
Exposure time: 59s
Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)
Negative control: kidney (PMID: 7545978, 7744035)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812) at 1/1000 dilution
Lane 1: Rat cerebral cortex tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Rat P5 brain tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Rat kidney tissue lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 48 kDa, 36 kDa
Exposure time: 136s
Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
All lanes: Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812) at 1/1000 dilution
Lane 1: Mouse brain nuclear fraction at 20 µg with 5% NFDM/TBST
Lane 2: Mouse brain non-nuclear fraction at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 48 kDa, 36 kDa, 15 kDa
Exposure time: 92s
Flow Cytometry (Intracellular) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (ab315812)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling MATH2/NEUROD6 with ab315812 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.