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AB315813

Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free

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Rabbit Recombinant Monoclonal MATH2/NEUROD6 antibody. Carrier free. Suitable for IHC-P, WB, IP, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Transfected cell line - Mouse, Transfected cell lysate - Mouse samples.

View Alternative Names

Ath2, Atoh2, Nex1, Neurod6, Neurogenic differentiation factor 6, NeuroD6, Helix-loop-helix protein mATH-2, Protein NEX-1, Protein atonal homolog 2, mATH2

15 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)

This data was developed using ab315812, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse NeuroD6 expression vector containing a Myc-His tag. (B) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling MATH2/NEUROD6 with ab315812 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on HEK-293T transfected with a Myc-His-tagged mouse NeuroD6 construct (image A). No staining on HEK-293T transfected with empty plasmid (image B). The section was incubated with ab315812 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)

This data was developed using ab315812, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling MATH2/NEUROD6 with ab315812 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse cardiac muscle (PMID : 7545978). The section was incubated with ab315812 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)

This data was developed using ab315812, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling MATH2/NEUROD6 with ab315812 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat lung. The section was incubated with ab315812 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)

This data was developed using ab315812, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling MATH2/NEUROD6 with ab315812 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse lung (PMID : 7545978). The section was incubated with ab315812 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)

This data was developed using ab315812, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling MATH2/NEUROD6 with ab315812 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat cardiac muscle. The section was incubated with ab315812 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)

This data was developed using ab315812, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling MATH2/NEUROD6 with ab315812 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebrum (PMID : 7545978). The section was incubated with ab315812 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)

This data was developed using ab315812, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MATH2/NEUROD6 with ab315812 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat cerebrum. The section was incubated with ab315812 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)

This data was developed using ab315812, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling MATH2/NEUROD6 with ab315812 at 1/50 (10.66 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).

Confocal image showing nuclear staining in mouse primary neurons.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

Immunocytochemistry/ Immunofluorescence - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)

This data was developed using ab315812, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling MATH2/NEUROD6 with ab315812 at 1/50 (10.66 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).

Confocal image showing nuclear staining in rat primary neuron.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

Flow Cytometry (Intracellular) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)

This data was developed using ab315812, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling MATH2/NEUROD6 with ab315812 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Immunoprecipitation - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)
  • IP

Supplier Data

Immunoprecipitation - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)

This data was developed using ab315812, the same antibody clone in a different buffer formulation.

MATH2/NEUROD6 was immunoprecipitated from 0.35 mg Mouse P5 cerebral cortex tissue lysate with ab315812 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315812 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (<a href='/en-us/products/primary-antibodies/math2-neurod6-antibody-epr28660-23-ab315812'>ab315812</a>) at 1/1000 dilution

Lane 1:

Mouse P5 cerebral cortex tissue lysate

Lane 2:

<a href='/en-us/products/primary-antibodies/math2-neurod6-antibody-epr28660-23-ab315812'>ab315812</a> at 1/30 IP in Mouse P5 cerebral cortex tissue lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/math2-neurod6-antibody-epr28660-23-ab315812'>ab315812</a> in Mouse P5 cerebral cortex tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)
  • WB

Supplier Data

Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)

This data was developed using ab315812, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : heart, lung (PMID : 7545978, 7744035)

The identity of the lower MW band at approximately 25 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (<a href='/en-us/products/primary-antibodies/math2-neurod6-antibody-epr28660-23-ab315812'>ab315812</a>) at 1/1000 dilution

Lane 1:

Mouse P5 cerebral cortex tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse lung tissue lysate at 20 µg

Lane 4:

Rat P5 hippocampus tissue lysate at 20 µg

Lane 5:

Rat heart tissue lysate at 20 µg

Lane 6:

Rat lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 48 kDa,36 kDa

false

Exposure time: 59s

Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)
  • WB

Supplier Data

Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)

This data was developed using ab315812, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : kidney (PMID : 7545978, 7744035)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (<a href='/en-us/products/primary-antibodies/math2-neurod6-antibody-epr28660-23-ab315812'>ab315812</a>) at 1/1000 dilution

Lane 1:

Rat cerebral cortex tissue lysate at 20 µg

Lane 2:

Rat P5 brain tissue lysate at 20 µg

Lane 3:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 48 kDa,36 kDa

false

Exposure time: 136s

Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)
  • WB

Supplier Data

Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)

This data was developed using ab315812, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

All lanes:

Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (<a href='/en-us/products/primary-antibodies/math2-neurod6-antibody-epr28660-23-ab315812'>ab315812</a>) at 1/1000 dilution

Lane 1:

Mouse brain nuclear fraction at 20 µg

Lane 2:

Mouse brain non-nuclear fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 48 kDa,36 kDa,15 kDa

false

Exposure time: 92s

Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)
  • WB

Supplier Data

Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] - BSA and Azide free (AB315813)

This data was developed using ab315812, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-MATH2/NEUROD6 antibody [EPR28660-23] (<a href='/en-us/products/primary-antibodies/math2-neurod6-antibody-epr28660-23-ab315812'>ab315812</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse NEUROD6 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 48 kDa,36 kDa

false

Exposure time: 37s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28660-23

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

ICC/IF, Flow Cyt (Intra), WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line - Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate - Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab315813 is the carrier-free version of ab315812.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MATH2 also known as NEUROD6 is important for the development and differentiation of neurons. It acts as a transcription factor regulating gene expression. With a molecular weight of approximately 39 kDa MATH2/NEUROD6 is expressed in the central nervous system particularly in the brain. This protein belongs to the basic helix-loop-helix (bHLH) family which are known for binding to specific DNA sequences to modulate transcription.
Biological function summary

MATH2/NEUROD6 plays a critical role in the nervous system. It influences neuronal survival outgrowth and synaptogenesis. Although not part of a larger protein complex it functions closely with other bHLH proteins to promote neurogenesis. Its similarity to NEUROD1 suggests overlap in their biological roles especially in the regulation of development in the nervous system.

Pathways

MATH2/NEUROD6 is key in the neurogenesis pathway facilitating the formation and specialization of neurons. It works alongside proteins such as MAPK which regulate various cellular activities including proliferation differentiation and response to extracellular stimuli. MATH2/NEUROD6 also interacts within the signaling pathways that affect neuronal differentiation aligning with other bHLH proteins for synaptic plasticity.

MATH2/NEUROD6 associates with neurodevelopmental disorders. Abnormal expression levels are linked to diseases such as Alzheimer's and Parkinson's due to their role in neuronal survival and plasticity. The protein's connection with NEUROD1 and its involvement in maintaining proper brain function underline its importance in research on neurological diseases.
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Product protocols

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Target data

Activates E box-dependent transcription in collaboration with TCF3/E47. May be a trans-acting factor involved in the development and maintenance of the mammalian nervous system. Transactivates the promoter of its own gene.
See full target information Neurod6
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