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AB251170

Anti-MATH5/ATOH7 antibody [EPR13935] - BSA and Azide free

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Rabbit Recombinant Monoclonal MATH5/ATOH7 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.

View Alternative Names

ATH5, BHLHA13, ATOH7, Transcription factor ATOH7, Atonal bHLH transcription factor 7, Class A basic helix-loop-helix protein 13, Protein atonal homolog 7, bHLHa13

1 Images
Western blot - Anti-MATH5/ATOH7 antibody [EPR13935] - BSA and Azide free (AB251170)
  • WB

Supplier Data

Western blot - Anti-MATH5/ATOH7 antibody [EPR13935] - BSA and Azide free (AB251170)

This data was developed using ab193356, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MATH5/ATOH7 antibody [EPR13935] (<a href='/en-us/products/primary-antibodies/math5-atoh7-antibody-epr13935-ab193356'>ab193356</a>) at 1/5000 dilution

Lane 1:

Mouse eyeball lysate at 20 µg

Lane 2:

Rat eyeball lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 16 kDa

Observed band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13935

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab251170 is the carrier-free version of ab193356.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MATH5 also known as ATOH7 is a basic helix-loop-helix transcription factor with an estimated mass of around 19 kDa. MATH5 is expressed mostly in the developing retina particularly in retinal ganglion cells. This protein acts as an important transcriptional regulator binding to DNA and initiating expression of specific genes necessary for eye development. MATH5's expression is tightly regulated during distinct stages of retinal development.
Biological function summary

MATH5/ATOH7 plays a vital role in the development of the retina by determining the fate of retinal progenitor cells. The protein acts as a part of a transcriptional network essential for specifying retinal ganglion cells. It functions without forming larger protein complexes but interacts with a variety of other elements to control gene expression. Its influence is critical for the progression of progenitor cells into differentiated retinal ganglion cells.

Pathways

MATH5/ATOH7 is integral to the neural developmental pathway particularly the ones involved in eye formation. It operates in conjunction with proteins like PAX6 and NEUROD1 which also have significant roles in the development of neural tissues. The signaling network that includes MATH5/ATOH7 directly influences the differentiation of retinal cells linking it to essential developmental pathways.

MATH5/ATOH7 deficiencies directly associate with congenital eye diseases such as optic nerve hypoplasia. Mutations in the ATOH7 gene can lead to improper development of the retinal ganglion cells often resulting in visual impairment. Furthermore MATH5/ATOH7's interaction with PAX6 can influence the risk of aniridia a condition that leads to a spectrum of eye abnormalities. Researchers study these interactions to better understand the genetic causes behind such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that binds to DNA at the consensus sequence 5'-CAG[GC]TG-3' (PubMed : 31696227). Dimerization with TCF3 isoform E47 may be required in certain situations (PubMed : 31696227). Binds to gene promoters and enhancer elements, and thereby regulates a transcriptional program of retinal ganglion cell (RGC) determinant genes (By similarity). Although the exact mechanism is not certain, retinal transcription regulation by ATOH7 has a role in RGC determination and survival, photoreceptor population development, targeting of RGC axons to the optic nerve and development of the retino-hypothalamic tract (By similarity). Binds to its own promoter and enhancer sequences, suggesting autoregulation of ATOH7 transcription (By similarity). Required for retinal circadian rhythm photoentrainment (By similarity). Plays a role in brainstem auditory signaling and binaural processing (By similarity).
See full target information ATOH7
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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