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AB84422

Anti-Matrin 3 antibody

5

(2 Reviews)

|

(6 Publications)

Rabbit Polyclonal Matrin 3 antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human MATR3 aa 800 to C-terminus.

View Alternative Names

KIAA0723, MATR3, Matrin-3

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (AB84422)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (AB84422)

Detection of Human Matrin 3 by Immunofluorescence.

Sample : FFPE section of human breast carcinoma.
Antibody : ab84422 used at a dilution of 1/100.
Detection : Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (AB84422)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (AB84422)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon adenocarcinoma using 1/250 ab84422, followed by DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (AB84422)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (AB84422)

Detection of Human Matrin 3 by Immunofluorescence.

Sample : FFPE section of Human breast carcinoma.
Antibody : ab84422 used at a dilution of 1/100.
Detection : Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (AB84422)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (AB84422)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse squamous cell carcinoma using 1/250 ab84422, followed by DAB staining.

Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody (AB84422)
  • ICC/IF

AbReview26054****

Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody (AB84422)

ab84422 staining Matrin 3 in human U2OS cells by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in paraformaldehyde, permeabilized using 0.5% NP-40, blocked with 1% BSA for 1 hour a room temperature, then incubated with ab84422 at a 1/100 dilution for 1 hour. The secondary antibody used was an Alexa-Fluor 488 conjugate donkey polyclonal, used at a 1/1000 dilution.

Image courtesy of Leilani Beltran by Abreview.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human MATR3 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P43243

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Note: expected to work on frozen sections.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Note: expected to work on frozen sections.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Bat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed : 28712728). Binds to N6-methyladenosine (m6A)-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed : 32245947). May bind to specific miRNA hairpins (PubMed : 28431233).
See full target information MATR3
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Biology 11: PubMed35205163

2022

Selective Loss of MATR3 in Spinal Interneurons, Upper Motor Neurons and Hippocampal CA1 Neurons in a MATR3 S85C Knock-In Mouse Model of Amyotrophic Lateral Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Justin You,Katarina Maksimovic,Jooyun Lee,Mashiat Khan,Rintaro Masuda,Jeehye Park

Nature communications 12:6241 PubMed34716321

2021

Inner nuclear protein Matrin-3 coordinates cell differentiation by stabilizing chromatin architecture.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Ji Cha,Özgün Uyan,Yan Kai,Tianxin Liu,Qian Zhu,Zuzana Tothova,Giovanni A Botten,Jian Xu,Guo-Cheng Yuan,Job Dekker,Stuart H Orkin

Acta neuropathologica communications 8:177 PubMed33129345

2020

RNA dependent suppression of C9orf72 ALS/FTD associated neurodegeneration by Matrin-3.

Applications

Unspecified application

Species

Unspecified reactive species

Nandini Ramesh,Elizabeth L Daley,Amanda M Gleixner,Jacob R Mann,Sukhleen Kour,Darilang Mawrie,Eric N Anderson,Julia Kofler,Christopher J Donnelly,Evangelos Kiskinis,Udai Bhan Pandey

Nature communications 11:5304 PubMed33082323

2020

Selective neuronal degeneration in MATR3 S85C knock-in mouse model of early-stage ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Ching Serena Kao,Rebekah van Bruggen,Jihye Rachel Kim,Xiao Xiao Lily Chen,Cadia Chan,Jooyun Lee,Woo In Cho,Melody Zhao,Claudia Arndt,Katarina Maksimovic,Mashiat Khan,Qiumin Tan,Michael D Wilson,Jeehye Park

FEBS letters 594:2800-2818 PubMed32515490

2020

Knockdown of genes involved in axonal transport enhances the toxicity of human neuromuscular disease-linked MATR3 mutations in Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Melody Zhao,Ching Serena Kao,Claudia Arndt,David Duc Tran,Woo In Cho,Katarina Maksimovic,Xiao Xiao Lily Chen,Mashiat Khan,Hongxian Zhu,Julia Qiao,Kailong Peng,Jingyao Hong,Jialu Xu,Deanna Kim,Jihye Rachel Kim,Jooyun Lee,Rebekah van Bruggen,Wan Hee Yoon,Jeehye Park

Molecular biology of the cell 27:3894-3902 PubMed27733621

2016

Identification of novel nesprin-1 binding partners and cytoplasmic matrin-3 in processing bodies.

Applications

ICC/IF, WB

Species

Human, Human

Dipen Rajgor,Jonathan G Hanley,Catherine M Shanahan
View all publications
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Product promise

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