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Knockout Tested Rabbit Recombinant Monoclonal Matrin 3 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 13 publications.


Images

Western blot - Anti-Matrin 3 antibody [EPR10635(B)] (AB151714), expandable thumbnail
  • Western blot - Anti-Matrin 3 antibody [EPR10635(B)] (AB151714), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (AB151714), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [EPR10635(B)] (AB151714), expandable thumbnail
  • Western blot - Anti-Matrin 3 antibody [EPR10635(B)] (AB151714), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Tested
Expected
Expected
Rat
Tested
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

For unpurified use at 1/250 - 1/500.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/2000
Notes

For unpurified use at 1/250 - 1/500.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/2000
Notes

For unpurified use at 1/250 - 1/500.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/10000 - 1/50000
Notes

-

Species
Rat
Dilution info
1/10000 - 1/50000
Notes

-

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250 - 1/1500
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/220
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728). Binds to N6-methyladenosine (m6A)-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed:32245947). May bind to specific miRNA hairpins (PubMed:28431233).

Alternative names

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal Matrin 3 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 13 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10635(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

  • Western blot - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714), expandable thumbnail

    Western blot - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    ab151714 was shown to react with MATR3 in wild-type HAP1 cells in Western blot with loss of signal observed in a MATR3 knockout cell line. Wild-type HAP1 and MATR3 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab151714 overnight at 4 °C at a 1/10000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2 μg/ml before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    Predicted band size: 95 kDa

  • Western blot - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714), expandable thumbnail

    Western blot - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    Blocking/Diluting buffer 5% NFDM/TBST

    All lanes: Western blot - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714) at 1/10000 dilution

    Lane 1: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 2: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

    Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 4: Mouse brain tissue lysate at 20 µg

    Lane 5: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 59 kDa, 95 kDa

    Observed band size: 125 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    Immunohistochemical analysis of paraffin-embedded human colon tissue sections labeling Matrin 3 with purified ab151714 at a dilution of 1/2000 (0.7 μg/ml). Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using citrate Buffer, pH 6.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Matrin 3 with purified ab151714 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 μg/mL) - Microtubule Marker (Alexa Fluor® 594) at 1/200. DAPI (blue) was used as a nuclear counterstain. Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Western blot - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714), expandable thumbnail

    Western blot - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    All lanes: Western blot - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714) at 1/1000 dilution

    Lane 1: HEK293T cell lysate at 10 µg

    Lane 2: K562 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: HepG2 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 95 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    Immunohistochemical analysis of Paraffin-embedded rat liver tissue sections labeling Matrin 3 with purified ab151714 at a dilution of 1/2000 (0.7 μg/ml). Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using citrate Buffer, pH 6.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue sections labeling Matrin 3 with purified ab151714 at a dilution of 1/2000 (0.7 μg/ml). Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using citrate Buffer, pH 6.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Flow Cytometry (Intracellular) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    Intracellular Flow Cytometry analysis of 4%paraformaldehyde fixedHepG2 (human hepatocellular carcinoma) cells labeling Matrin 3 with purified ab151714 at a dilution of 1/220. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Matrin 3 with unpurified ab151714 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Matrin 3 with unpurified ab151714 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    Immunofluorescent analysis of HepG2 cells labeling Matrin 3 with unpurified ab151714 at 1/250 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using unpurified ab151714 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using unpurified ab151714 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using unpurified ab151714 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

    ab151714 was shown to react with MATR3 in wild-type HAP1 cells in immunocytochemistry with loss of signal observed in a MATR3 knockout cell line. Wild-type and knockout cells were mixed and pelleted at a 1:1 ratio on coverslips. The cells were fixed with 4% paraformaldehyde (15 min) then permeabilized with 0.1% Triton X-100 (10min) and then blocked with 1x PBS, 0.01% Triton X-100, 5% BSA, 5% NGS. The cells were then incubated with ab151714 at dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat anti-rabbit secondary antibody to (Alexa Fluor® 555) at 0.5 ?g/ml. Acquisition of the green (wild-type), red (antibody staining) and far-red (knockout) channels was performed. Representative grayscale images of the red channel are shown. Wild-type and knockout cells are outlined with yellow and magenta dashed line, respectively. Schematic representation of the mosaic strategy used is shown on the bottom-right panel. Image was acquired with a Zeiss(LSM-880).

    This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

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