Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric)

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (AB281927)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling Matrin-3 with ab281927 at 1/50 (22.16 μg/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line.

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab281927 at a 1/50 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/500 dilution.

Negative control 2 : ab179513 Anti-beta Tubulin rabbit monoclonal antibody at a 1/200 dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (AB281927)

Matrin-3 was immunoprecipitated from 0.35 mg K562 (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with ab281927 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab281927 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/1000 dilution.

Lane 1 : K562 whole cell lysate 10 μg

Lane 2 : ab281927 IP in K562 whole cell lysate

Lane 3 : Mouse IgG2a (ab18413) instead of ab281927 in K562 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (ab281927)

Predicted band size: 95 kDa

Observed band size: 120 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (AB281927)

Matrin-3 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with ab281927 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab281927 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/1000 dilution.

Lane 1 : HeLa whole cell lysate 10 μg

Lane 2 : ab281927 IP in HeLa whole cell lysate

Lane 3 : Mouse IgG2a (ab18413) instead of ab281927 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (ab281927)

Predicted band size: 95 kDa

Observed band size: 120 kDa

false

• WB

Lab

Western blot - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (AB281927)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 26878116).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 84 seconds.

All lanes:

Western blot - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (ab281927) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 95 kDa

Observed band size: 120 kDa

false