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Mouse Recombinant Monoclonal Matrin 3 antibody. Suitable for ICC/IF, IP, WB and reacts with Human samples.

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Images

Western blot - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (AB281927), expandable thumbnail
  • Immunoprecipitation - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (AB281927), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (AB281927), expandable thumbnail
  • Immunoprecipitation - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (AB281927), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWB
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728). Binds to N6-methyladenosine (m6A)-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed:32245947). May bind to specific miRNA hairpins (PubMed:28431233).

Alternative names

KIAA0723, MATR3, Matrin-3

Recommended products

Mouse Recombinant Monoclonal Matrin 3 antibody. Suitable for ICC/IF, IP, WB and reacts with Human samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Clone number
Z-MATR3-4
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

ab281927 is a mouse monoclonal chimeric antibody.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody was developed by the Structural Genomics Consortium and manufactured by Abcam.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (ab281927), expandable thumbnail

    Western blot - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (ab281927)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 26878116).

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 84 seconds.

    All lanes: Western blot - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (ab281927) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 2: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 95 kDa

    Observed band size: 120 kDa

  • Immunoprecipitation - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (ab281927), expandable thumbnail

    Immunoprecipitation - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (ab281927)

    Matrin-3 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with ab281927 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab281927 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg

    Lane 2: ab281927 IP in HeLa whole cell lysate

    Lane 3: Mouse IgG2a (Mouse IgG2a, Kappa Monoclonal [MOPC-173] -Isotype Control - ChIP Grade ab18413) instead of ab281927 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (ab281927)

    Predicted band size: 95 kDa

    Observed band size: 120 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (ab281927), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (ab281927)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling Matrin-3 with ab281927 at 1/50 (22.16 μg/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line.

    Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab281927 at a 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution.

    Negative control 2: Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody at a 1/200 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (ab281927), expandable thumbnail

    Immunoprecipitation - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (ab281927)

    Matrin-3 was immunoprecipitated from 0.35 mg K562 (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with ab281927 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab281927 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.

    Lane 1: K562 whole cell lysate 10 μg

    Lane 2: ab281927 IP in K562 whole cell lysate

    Lane 3: Mouse IgG2a (Mouse IgG2a, Kappa Monoclonal [MOPC-173] -Isotype Control - ChIP Grade ab18413) instead of ab281927 in K562 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-Matrin 3 antibody [Z-MATR3-4] - Mouse IgG2a (Chimeric) (ab281927)

    Predicted band size: 95 kDa

    Observed band size: 120 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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