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AB28287

Anti-Matriptase 2 antibody

5

(5 Reviews)

|

(2 Publications)

Rabbit Polyclonal Matriptase 2 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TMPRSS6.

View Alternative Names

UNQ354/PRO618, TMPRSS6, Transmembrane protease serine 6, Matriptase-2

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Matriptase 2 antibody (AB28287)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Matriptase 2 antibody (AB28287)

ICC/IF image of ab28287 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28287, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human TMPRSS6. The exact immunogen used to generate this antibody is proprietary information.

Q8IU80

Specificity

ab28287 recognises the stem region of Matriptase 2. It does not react with the free enzyme. It does not recognise Matriptase 1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Membrane-bound serine protease (PubMed : 18976966, PubMed : 20518742, PubMed : 25156943, PubMed : 25588876). Through the cleavage of cell surface HJV, a regulator of the expression of the iron absorption-regulating hormone hepicidin/HAMP, plays a role in iron homeostasis (PubMed : 18408718, PubMed : 18976966, PubMed : 25156943).
See full target information TMPRSS6
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell chemical biology 26:1559-1572.e9 PubMed31543462

2019

Discovery and Development of TMPRSS6 Inhibitors Modulating Hepcidin Levels in Human Hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

François Béliveau,Aarti Tarkar,Sébastien P Dion,Antoine Désilets,Mariana Gabriela Ghinet,Pierre-Luc Boudreault,Catherine St-Georges,Éric Marsault,Daniel Paone,Jon Collins,Colin H Macphee,Nino Campobasso,Arthur Groy,Josh Cottom,Michael Ouellette,Andrew J Pope,Richard Leduc

Molecular vision 20:561-74 PubMed24791141

2014

Retinal expression of the serine protease matriptase-2 (Tmprss6) and its role in retinal iron homeostasis.

Applications

Unspecified application

Species

Mouse

Jaya P Gnana-Prakasam,Renee B Baldowski,Sudha Ananth,Pamela M Martin,Sylvia B Smith,Vadivel Ganapathy
View all publications
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Product promise

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