AB22396

Anti-Matrix protein 1 antibody [GA2B]

Name: Anti-Matrix protein 1 antibody [GA2B] (ab22396) | Abcam
Brand: Abcam Limited
SKU: AB22396
Price: 435 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(27 Publications)

Mouse Monoclonal M1 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt and reacts with Influenza A samples. Cited in 27 publications. Immunogen corresponding to Virus preparation containing Matrix protein 1 protein.

View Alternative Names

Matrix protein 1, M1, M

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Matrix protein 1 antibody [GA2B] (AB22396)

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-Matrix protein 1 antibody [GA2B] (AB22396)

Immunofluorescence analysis of HeLa cells staining Influenza A Virus M1 using ab22396.

Cells were treated with either 20 µg/ml Prostaglandin A (PGA) or EtOH vehicle control, 3 hours post infection by Influenza A Virus, then fractionated at 9 hours post infection before analysis by immunofluorescence.

Image from Chase GP et al., PLoS Pathog. 2011 Sep;7(9):e1002187. Epub 2011 Sep 1.Fig 4.; doi:10.1371/journal.ppat.1002187; September 1, 2011, PLoS Pathog 7(9): e1002187.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GA2B

Isotype

IgG1

Carrier free

Reacts with

Influenza A

Applications

Flow Cyt, ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

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Properties and storage information

Form

Liquid

Purity

undefined SDS-PAGE

Purification notes

>90% IgG content as established by SDS PAGE

Storage buffer

pH: 7.5 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays critical roles in virus replication, from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex, which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1, a non-raft-associated protein, into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion, where it binds the RNP. During virus entry into cell, the M2 ion channel acidifies the internal virion core, inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus, where viral transcription and replication can take place.. Determines the virion's shape : spherical or filamentous. Clinical isolates of influenza are characterized by the presence of significant proportion of filamentous virions, whereas after multiple passage on eggs or cell culture, virions have only spherical morphology. Filamentous virions are thought to be important to infect neighboring cells, and spherical virions more suited to spread through aerosol between hosts organisms.

See full target information M

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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

NPJ vaccines 8:132 PubMed37679361

2023

A mosaic influenza virus-like particles vaccine provides broad humoral and cellular immune responses against influenza A viruses.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejie Liu,Tianyi Zhao,Liangliang Wang,Zhuolin Yang,Chuming Luo,Minchao Li,Huanle Luo,Caijun Sun,Huacheng Yan,Yuelong Shu

Signal transduction and targeted therapy 8:194 PubMed37160897

2023

SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication.

Applications

Unspecified application

Species

Unspecified reactive species

Pan Pan,Weiwei Ge,Zhiwei Lei,Wei Luo,Yuqing Liu,Zhanwen Guan,Lumiao Chen,Zhenyang Yu,Miaomiao Shen,Dingwen Hu,Qi Xiang,Wenbiao Wang,Pin Wan,Mingfu Tian,Yang Yu,Zhen Luo,Xulin Chen,Heng Xiao,Qiwei Zhang,Xujing Liang,Xin Chen,Yongkui Li,Jianguo Wu

The Journal of biological chemistry 298:102698 PubMed36379255

2022

The E3 ligase subunit FBXO45 binds the interferon-λ receptor and promotes its degradation during influenza virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

MuChun Tsai,Wissam Osman,Jessica Adair,Rabab ElMergawy,Lexie Chafin,Finny Johns,Daniela Farkas,Ajit Elhance,James Londino,Rama K Mallampalli

Nature communications 13:5841 PubMed36192426

2022

Orthogonally-tunable and ER-targeting fluorophores detect avian influenza virus early infection.

Applications

Unspecified application

Species

Unspecified reactive species

Taewon Kang,Md Mamunul Haque,Boran Lee,Kyung Tae Hong,Seong Cheol Hong,Younghun Kim,Jesang Lee,Jun-Seok Lee,Dongwhan Lee

PLoS pathogens 18:e1010755 PubMed36006890

2022

Universal protection against influenza viruses by multi-subtype neuraminidase and M2 ectodomain virus-like particle.

Applications

Unspecified application

Species

Unspecified reactive species

Ki-Hye Kim,Zhuo Li,Noopur Bhatnagar,Jeeva Subbiah,Bo Ryoung Park,Chong Hyun Shin,Peter Pushko,Bao-Zhong Wang,Sang-Moo Kang

Viruses 14: PubMed35458460

2022

Characterization of Immune Response towards Generation of Universal Anti-HA-Stalk Antibodies after Immunization of Broiler Hens with Triple H5N1/NA-HA-M1 VLPs.

Applications

Unspecified application

Species

Unspecified reactive species

Beata Gromadzka,Milena Chraniuk,Lilit Hovhannisyan,Karolina Uranowska,Bogusław Szewczyk,Magdalena Narajczyk,Mirosława Panasiuk

Frontiers in immunology 12:735576 PubMed34899695

2021

Interferon Lambda Signaling in Macrophages Is Necessary for the Antiviral Response to Influenza.

Applications

Unspecified application

Species

Unspecified reactive species

Rama K Mallampalli,Jessica Adair,Ajit Elhance,Daniela Farkas,Lexie Chafin,Matthew E Long,Mithu De,Ana L Mora,Mauricio Rojas,Victor Peters,Joseph S Bednash,MuChun Tsai,James D Londino

Molecular immunology 114:149-161 PubMed31352231

2019

Cross-clade antibody reactivity may attenuate the ability of influenza virus to evade the immune response.

Applications

Unspecified application

Species

Unspecified reactive species

Sharad Vashisht,Sheenam Verma,Dinakar M Salunke

Frontiers in microbiology 10:1630 PubMed31379782

2019

Influenza Vaccine With Consensus Internal Antigens as Immunogens Provides Cross-Group Protection Against Influenza A Viruses.

Applications

Unspecified application

Species

Unspecified reactive species

Xinci Xie,Chen Zhao,Qian He,Tianyi Qiu,Songhua Yuan,Longfei Ding,Lu Liu,Lang Jiang,Jing Wang,Linxia Zhang,Chao Zhang,Xiang Wang,Dongming Zhou,Xiaoyan Zhang,Jianqing Xu

PloS one 14:e0216871 PubMed31246961

2019

Influenza M2 virus-like particle vaccination enhances protection in combination with avian influenza HA VLPs.

Applications

Unspecified application

Species

Unspecified reactive species

Hae-Ji Kang,Ki-Back Chu,Dong-Hun Lee,Su-Hwa Lee,Bo Ryoung Park,Min-Chul Kim,Sang-Moo Kang,Fu-Shi Quan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Matrix protein 1 antibody [GA2B]

Immunocytochemistry/ Immunofluorescence - Anti-Matrix protein 1 antibody [GA2B] (AB22396)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (27)

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