Anti-MAVS antibody [EPR26357-91]

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MAVS antibody [EPR26357-91] (AB290729)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) cells labelling MAVS with ab290729 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAVS antibody [EPR26357-91] (AB290729)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcin tissue labeling MAVS with ab290729 at 1/500 (1.032 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on human ovarian carcinoma (PMID : 34722508).The section was incubated with ab290729 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAVS antibody [EPR26357-91] (AB290729)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling MAVS with ab290729 at 1/50 (10.32 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing mitochondrial staining in the 293T cell line is observed. ab33985 Anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/1000 1 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAVS antibody [EPR26357-91] (AB290729)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling MAVS with ab290729 at 1/500 (1.032 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on human skeletal muscle (PMID : 16153868).The section was incubated with ab290729 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAVS antibody [EPR26357-91] (AB290729)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MAVS with ab290729 at 1/500 (1.032 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on human cardiac muscle (PMID : 16153868).The section was incubated with ab290729 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAVS antibody [EPR26357-91] (AB290729)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling MAVS with ab290729 at 1/500 (1.032 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on human lung carcinoma (PMID : 29285267).The section was incubated with ab290729 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-MAVS antibody [EPR26357-91] (AB290729)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The molecular weight observed is consistent with that described in the literature (PMID : 16125763 and 30460894) Lysates were prepared from fresh materials and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-MAVS antibody [EPR26357-91] (ab290729) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell fresh lysate 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell fresh lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa,52 kDa,37 kDa,30 kDa

false

Exposure time: 37s

• WB

Lab

Western blot - Anti-MAVS antibody [EPR26357-91] (AB290729)

Western blot : Anti-MAVS antibody [EPR26357-91] ab290729 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 70 kDa in Wild-type A549 cell lysates with no signal observed at this size in MAVS knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-MAVS antibody [EPR26357-91] (ab290729) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysates at 20 µg

Lane 2:

Western blot - Human MAVS knockout A549 cell line (<a href='/en-us/products/cell-lines/human-mavs-knockout-a549-cell-line-ab282354'>ab282354</a>) at 20 µg

Lane 3:

HEK-293 cell lysates at 20 µg

Lane 4:

A-431 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 70 kDa

Observed band size: 70 kDa

false