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Rabbit Recombinant Monoclonal MAVS antibody. Carrier free. Suitable for Flow Cyt (Intra), IHC-P, ICC/IF, WB and reacts with Mouse samples.

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Images

Flow Cytometry (Intracellular) - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (AB314445), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (AB314445), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (AB314445), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (AB314445), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (AB314445), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)IHC-PICC/IFWBIP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

Target data

Alternative names

Mitochondrial antiviral-signaling protein, MAVS, CARD adapter inducing interferon beta, Interferon beta promoter stimulator protein 1, Virus-induced-signaling adapter, Cardif, IPS-1, VISA, Visa, Ips1, Mavs

Recommended products

Rabbit Recombinant Monoclonal MAVS antibody. Carrier free. Suitable for Flow Cyt (Intra), IHC-P, ICC/IF, WB and reacts with Mouse samples.

Alternative names

Mitochondrial antiviral-signaling protein, MAVS, CARD adapter inducing interferon beta, Interferon beta promoter stimulator protein 1, Virus-induced-signaling adapter, Cardif, IPS-1, VISA, Visa, Ips1, Mavs

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR28262-28

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab314445 is the carrier-free version of Anti-MAVS antibody [EPR28262-28] ab314444.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MAVS also known as mitochondrial antiviral-signaling protein is a critical adaptor protein involved in the innate immune response to viral infections. This protein with a molecular weight of approximately 56 kDa is expressed on the mitochondrial membrane. It plays a major role in antiviral defense by transmitting signals from cytosolic pattern recognition receptors like RIG-I-like receptors to initiate downstream immune responses. MAVS can also be referred to as IPS-1 VISA or CARDIF in scientific literature.

Biological function summary

The MAVS protein activates important signaling cascades to produce type I interferons and other cytokines which are essential in the antiviral response. MAVS forms a complex with other proteins on the mitochondrial membrane helping to coordinate a rapid immune reaction to viral pathogens. It acts by amplifying the signal from RIG-I and MDA5 facilitating their role in the recognition of viral RNA. By recruiting downstream signaling molecules MAVS enables the activation of transcription factors like IRF3 and NF-kB.

Pathways

MAVS plays a central role in the signaling pathways that regulate innate immunity including the RIG-I-like receptor signaling pathway and the NF-kB pathway. It interacts with various proteins such as TRIF and STING in the pathways to modulate immune responses. These pathways help trigger the production of antiviral substances and maintain homeostasis within the body's defense mechanisms. MAVS provides a platform for the assembly of signaling complexes which are necessary for the propagation and amplification of the immune response signal.

Associated diseases and disorders

MAVS is associated with conditions such as viral infections and autoimmune diseases. Dysregulation of MAVS activity has been linked to systemic lupus erythematosus where abnormal immune signaling may occur. MAVS also has connections with other proteins such as TRAF3 and TRAF6 which contribute to disease pathogenesis. Understanding the role of MAVS in these conditions may provide insights into therapeutic targets for improving disease outcomes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Flow Cytometry (Intracellular) - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (ab314445), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (ab314445)

    This data was developed using Anti-MAVS antibody [EPR28262-28] ab314444, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (mouse myoblast) cells labelling MAVS with Anti-MAVS antibody [EPR28262-28] ab314444 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (ab314445), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (ab314445)

    This data was developed using Anti-MAVS antibody [EPR28262-28] ab314444, the same antibody clone in a different buffer formulation.
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblast) cells labelling MAVS with Anti-MAVS antibody [EPR28262-28] ab314444 at 1/50 (10.46 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in C2C12 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain at 1/50 (5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (ab314445), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (ab314445)

    This data was developed using Anti-MAVS antibody [EPR28262-28] ab314444, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse liver cancer tissue labeling MAVS with Anti-MAVS antibody [EPR28262-28] ab314444 at 1/500 (1.046 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse liver cancer. The section was incubated with Anti-MAVS antibody [EPR28262-28] ab314444 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (ab314445), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (ab314445)

    This data was developed using Anti-MAVS antibody [EPR28262-28] ab314444, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling MAVS with Anti-MAVS antibody [EPR28262-28] ab314444 at 1/500 (1.046 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse kidney. The section was incubated with Anti-MAVS antibody [EPR28262-28] ab314444 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (ab314445), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (ab314445)

    This data was developed using Anti-MAVS antibody [EPR28262-28] ab314444, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling MAVS with Anti-MAVS antibody [EPR28262-28] ab314444 at 1/500 (1.046 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse testis. The section was incubated with Anti-MAVS antibody [EPR28262-28] ab314444 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (ab314445), expandable thumbnail

    Western blot - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (ab314445)

    This data was developed using Anti-MAVS antibody [EPR28262-28] ab314444, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
    Exposure time: Lane 1-3: 180 seconds, Lane 4: 48 seconds.

    All lanes: Western blot - Anti-MAVS antibody [EPR28262-28] (Anti-MAVS antibody [EPR28262-28] ab314444) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse testis tissue lysate at 20 µg

    Lane 3: C2C12 (mouse myoblast) whole cell lysate at 20 µg

    Lane 4: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 75 kDa

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
    Exposure time: Lane 1-3: 180 seconds, Lane 4: 48 seconds.

  • Western blot - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (ab314445), expandable thumbnail

    Western blot - Anti-MAVS antibody [EPR28262-28] - BSA and Azide free (ab314445)

    This data was developed using Anti-MAVS antibody [EPR28262-28] ab314444, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-MAVS antibody [EPR28262-28] (Anti-MAVS antibody [EPR28262-28] ab314444) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 60 µg

    Lane 2: NIH/3T3 transfected with siRNA specifically targeting MAVS whole cell lysate at 60 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 75 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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