Mouse Monoclonal MAX antibody. Suitable for ChIP, Flow Cyt, Dot, WB and reacts with Human, Recombinant fragment, Recombinant full length protein - Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAX.
Images
![Western blot - Anti-MAX antibody [73C5a] (AB53570), expandable thumbnail](https://content.abcam.com/products/images/max-antibody-73c5a-ab53570--western-blot-img98704.gif "Western blot - Anti-MAX antibody [73C5a] (AB53570)")
![Flow Cytometry - Anti-MAX antibody [73C5a] (AB53570), expandable thumbnail](https://content.abcam.com/products/images/max-antibody-73c5a-ab53570--flow-cytometry-img80177.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-MAX antibody [73C5a] (AB53570)")
Publications
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- Communications biology 6:8722023The ABL-MYC axis controls WIPI1-enhanced autophagy in lifespan extension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatharina Sporbeck,Maximilian L Haas,Carmen J Pastor-Maldonado,David S Schüssele,Catherine Hunter,Zsuzsanna Takacs,Ana L Diogo de Oliveira,Mirita Franz-Wachtel,Chara Charsou,Simon G Pfisterer,Andrea Gubas,Patricia K Haller,Roland L Knorr,Manuel Kaulich,Boris Macek,Eeva-Liisa Eskelinen,Anne Simonsen,Tassula Proikas-CezannePubMed 37620393
- Nature communications 12:40912021Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun-Fan Sun,Liang Wu,Shi-Ping Liu,Miao-Miao Jiang,Bo Hu,Kai-Qian Zhou,Wei Guo,Yang Xu,Yu Zhong,Xiao-Rui Zhou,Ze-Fan Zhang,Geng Liu,Sheng Liu,Ying-Hong Shi,Yuan Ji,Min Du,Nan-Nan Li,Gui-Bo Li,Zhi-Kun Zhao,Xiao-Yun Huang,Li-Qin Xu,Qi-Chao Yu,David H Peng,Shuang-Jian Qiu,Hui-Chuan Sun,Michael Dean,Xiang-Dong Wang,Wen-Yuan Chung,Ashley R Dennison,Jian Zhou,Yong Hou,Jia Fan,Xin-Rong YangPubMed 34215748
- FEBS letters 594:3395-34052020Transcription of carbonyl reductase 1 is regulated by DNA topoisomerase II beta.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMushtaq M Khazeem,Ian G Cowell,Lauren F Harkin,John W Casement,Caroline A AustinPubMed 32767399
- International journal of biological sciences 16:1071-10852020The CARM1-p300-c-Myc-Max (CPCM) transcriptional complex regulates the expression of and affects the stability of CRL4 E3 ligases in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenzhu Lu et. al.PubMed 32140074
- PloS one 14:e02162182019Epigenetic control of the angiotensin-converting enzyme in endothelial cells during inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas Mudersbach et. al.PubMed 31042763
- Cell reports 25:2083-2093.e42018The Molecular Signature of Megakaryocyte-Erythroid Progenitors Reveals a Role for the Cell Cycle in Fate Specification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Chien Lu,Chad Sanada,Juliana Xavier-Ferrucio,Lin Wang,Ping-Xia Zhang,H Leighton Grimes,Meenakshi Venkatasubramanian,Kashish Chetal,Bruce Aronow,Nathan Salomonis,Diane S KrausePubMed 30463007
- Leukemia 33:1-142018Genetic predisposition to B-cell acute lymphoblastic leukemia at 14q11.2 is mediated by a CEBPE promoter polymorphism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames B Studd et. al.PubMed 29977016
- The Journal of biological chemistry 292:13333-133442017Arginine methylation regulates c-Myc-dependent transcription by altering promoter recruitment of the acetyltransferase p300.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrina Tikhanovich et. al.PubMed 28652407
- Nature communications 7:114782016Common genetic variation in ETV6 is associated with colorectal cancer susceptibility.
- Oncogene 35:5489-55002016Targeting soluble CD146 with a neutralizing antibody inhibits vascularization, growth and survival of CD146-positive tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Stalin et. al.PubMed 27065325
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human MAX. The exact immunogen used to generate this antibody is proprietary information. Database link P61244
Reactivity data
Select an application| ChIP | Flow Cyt | Dot | WB | |
|---|---|---|---|---|
| Human | Expected | Tested | Predicted | Expected |
| Recombinant fragment | Not recommended | Not recommended | Expected | Not recommended |
| Recombinant full length protein - Human | Not recommended | Not recommended | Not recommended | Tested |
ChIP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Recombinant full length protein - Human | Dilution info - | Notes - |
Dot
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements.
Alternative names
BHLHD4, MAX, Protein max, Class D basic helix-loop-helix protein 4, Myc-associated factor X, bHLHd4
Recommended products
Mouse Monoclonal MAX antibody. Suitable for ChIP, Flow Cyt, Dot, WB and reacts with Human, Recombinant fragment, Recombinant full length protein - Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAX.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human MAX. The exact immunogen used to generate this antibody is proprietary information. Database link P61244
- Clone number
- 73C5a
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
ab53570 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MAX protein also known as Myc-associated factor X functions mechanically as a transcription factor with a molecular weight of approximately 20 kDa. MAX is expressed in a wide range of tissues throughout the body. The protein forms homodimers or heterodimers with other members of the basic-helix-loop-helix-zipper (bHLHZ) family such as MYC MAD and MNT. The dimerization with different partners determines its role as an activator or repressor of transcription affecting the regulation of various genes.
- Biological function summary
The MAX protein plays a significant role in controlling cell growth differentiation and apoptosis. The protein often forms part of complex regulatory networks within the cell. By interacting with different partner proteins MAX modulates transcriptional activity affecting cellular outcomes. Importantly it can switch between activating or repressing target genes depending on the dimer partner influencing processes such as proliferation and differentiation.
- Pathways
MAX is deeply integrated into the MYC-MAX-MAD pathway an important pathway for regulating gene expression linked to cell cycle and proliferative processes. Within this pathway MAX pairs with either MYC or MAD depending on the cellular context to influence transcription. When paired with MYC it promotes transcription while pairing with MAD results in transcriptional repression. Additionally in the growth signaling pathway the MAX protein’s regulation of target genes is pivotal for ensuring proper cellular responses to growth signals.
- Associated diseases and disorders
The malfunction of the MAX protein has a strong association with cancer and neurodegenerative diseases. Alterations in MAX function can lead to uncontrolled cell growth contributing to oncogenesis particularly in relation to MYC which is a known oncogene. Furthermore dysregulation of the MAX protein is linked to neurodegenerative disorders where abnormal MAX activity may disrupt normal neural gene expression enhancing disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-MAX antibody [73C5a] (ab53570)
MAX Western blot staining of immunised recombinant protein using mouse Anti-MAX antibody
All lanes: Western blot - Anti-MAX antibody [73C5a] (ab53570)
All lanes: immunised recombinant protein
Predicted band size: 18 kDa
Observed band size: 34 kDa
![Flow Cytometry - Anti-MAX antibody [73C5a] (ab53570), expandable thumbnail](https://content.abcam.com/products/images/max-antibody-73c5a-ab53570--flow-cytometry-img80177.jpg "Flow Cytometry - Anti-MAX antibody [73C5a] (ab53570)")
Flow Cytometry - Anti-MAX antibody [73C5a] (ab53570)
Overlay histogram showing A549 cells stained with ab53570 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53570, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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