AB53570

Anti-MAX antibody [73C5a]

Name: Anti-MAX antibody [73C5a] (ab53570) | Abcam
Brand: Abcam Limited
SKU: AB53570
Price: 500 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(14 Publications)

Mouse Monoclonal MAX antibody. Suitable for ChIP, Flow Cyt, Dot, WB and reacts with Human, Recombinant fragment, Recombinant full length protein - Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAX.

View Alternative Names

BHLHD4, MAX, Protein max, Class D basic helix-loop-helix protein 4, Myc-associated factor X, bHLHd4

2 Images

Flow Cytometry - Anti-MAX antibody [73C5a] (AB53570)

Flow Cyt

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Flow Cytometry - Anti-MAX antibody [73C5a] (AB53570)

Overlay histogram showing A549 cells stained with ab53570 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53570, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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Western blot - Anti-MAX antibody [73C5a] (AB53570)

All lanes:

Western blot - Anti-MAX antibody [73C5a] (ab53570)

All lanes:

immunised recombinant protein

Predicted band size: 18 kDa

Observed band size: 34 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

73C5a

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

ChIP, Flow Cyt, WB, Dot

applications

Immunogen

Recombinant Fragment Protein within Human MAX. The exact immunogen used to generate this antibody is proprietary information.

P61244

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab53570 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MAX protein also known as Myc-associated factor X functions mechanically as a transcription factor with a molecular weight of approximately 20 kDa. MAX is expressed in a wide range of tissues throughout the body. The protein forms homodimers or heterodimers with other members of the basic-helix-loop-helix-zipper (bHLHZ) family such as MYC MAD and MNT. The dimerization with different partners determines its role as an activator or repressor of transcription affecting the regulation of various genes.

Biological function summary

The MAX protein plays a significant role in controlling cell growth differentiation and apoptosis. The protein often forms part of complex regulatory networks within the cell. By interacting with different partner proteins MAX modulates transcriptional activity affecting cellular outcomes. Importantly it can switch between activating or repressing target genes depending on the dimer partner influencing processes such as proliferation and differentiation.

Pathways

MAX is deeply integrated into the MYC-MAX-MAD pathway an important pathway for regulating gene expression linked to cell cycle and proliferative processes. Within this pathway MAX pairs with either MYC or MAD depending on the cellular context to influence transcription. When paired with MYC it promotes transcription while pairing with MAD results in transcriptional repression. Additionally in the growth signaling pathway the MAX protein’s regulation of target genes is pivotal for ensuring proper cellular responses to growth signals.

The malfunction of the MAX protein has a strong association with cancer and neurodegenerative diseases. Alterations in MAX function can lead to uncontrolled cell growth contributing to oncogenesis particularly in relation to MYC which is a known oncogene. Furthermore dysregulation of the MAX protein is linked to neurodegenerative disorders where abnormal MAX activity may disrupt normal neural gene expression enhancing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC : MAX complex is a transcriptional activator, whereas the MAD : MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements.

See full target information MAX

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:111829 PubMed39967876

2025

Functional variant at 19q13.3 confers nonsyndromic cleft palate susceptibility by regulating .

Applications

Unspecified application

Species

Unspecified reactive species

Shu Lou,Ziyue Miao,Xiaofeng Li,Lan Ma,Dandan Li,Mulong Du,Lin Wang,Yongchu Pan

Communications biology 6:872 PubMed37620393

2023

The ABL-MYC axis controls WIPI1-enhanced autophagy in lifespan extension.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Sporbeck,Maximilian L Haas,Carmen J Pastor-Maldonado,David S Schüssele,Catherine Hunter,Zsuzsanna Takacs,Ana L Diogo de Oliveira,Mirita Franz-Wachtel,Chara Charsou,Simon G Pfisterer,Andrea Gubas,Patricia K Haller,Roland L Knorr,Manuel Kaulich,Boris Macek,Eeva-Liisa Eskelinen,Anne Simonsen,Tassula Proikas-Cezanne

Nature communications 12:4091 PubMed34215748

2021

Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Fan Sun,Liang Wu,Shi-Ping Liu,Miao-Miao Jiang,Bo Hu,Kai-Qian Zhou,Wei Guo,Yang Xu,Yu Zhong,Xiao-Rui Zhou,Ze-Fan Zhang,Geng Liu,Sheng Liu,Ying-Hong Shi,Yuan Ji,Min Du,Nan-Nan Li,Gui-Bo Li,Zhi-Kun Zhao,Xiao-Yun Huang,Li-Qin Xu,Qi-Chao Yu,David H Peng,Shuang-Jian Qiu,Hui-Chuan Sun,Michael Dean,Xiang-Dong Wang,Wen-Yuan Chung,Ashley R Dennison,Jian Zhou,Yong Hou,Jia Fan,Xin-Rong Yang

FEBS letters 594:3395-3405 PubMed32767399

2020

Transcription of carbonyl reductase 1 is regulated by DNA topoisomerase II beta.

Applications

Unspecified application

Species

Unspecified reactive species

Mushtaq M Khazeem,Ian G Cowell,Lauren F Harkin,John W Casement,Caroline A Austin

International journal of biological sciences 16:1071-1085 PubMed32140074

2020

The CARM1-p300-c-Myc-Max (CPCM) transcriptional complex regulates the expression of and affects the stability of CRL4 E3 ligases in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenzhu Lu,Chunmei Yang,Hongbo He,Hong Liu

PloS one 14:e0216218 PubMed31042763

2019

Epigenetic control of the angiotensin-converting enzyme in endothelial cells during inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Mudersbach,Daniel Siuda,Karin Kohlstedt,Ingrid Fleming

Cell reports 25:2083-2093.e4 PubMed30463007

2018

The Molecular Signature of Megakaryocyte-Erythroid Progenitors Reveals a Role for the Cell Cycle in Fate Specification.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chien Lu,Chad Sanada,Juliana Xavier-Ferrucio,Lin Wang,Ping-Xia Zhang,H Leighton Grimes,Meenakshi Venkatasubramanian,Kashish Chetal,Bruce Aronow,Nathan Salomonis,Diane S Krause

Leukemia 33:1-14 PubMed29977016

2018

Genetic predisposition to B-cell acute lymphoblastic leukemia at 14q11.2 is mediated by a CEBPE promoter polymorphism.

Applications

Unspecified application

Species

Unspecified reactive species

James B Studd,Minjun Yang,Zhenhua Li,Jayaram Vijayakrishnan,Yi Lu,Allen Eng-Juh Yeoh,Kajsa Paulsson,Richard S Houlston

The Journal of biological chemistry 292:13333-13344 PubMed28652407

2017

Arginine methylation regulates c-Myc-dependent transcription by altering promoter recruitment of the acetyltransferase p300.

Applications

Unspecified application

Species

Unspecified reactive species

Irina Tikhanovich,Jie Zhao,Brian Bridges,Sean Kumer,Ben Roberts,Steven A Weinman

Nature communications 7:11478 PubMed27145994

2016

Common genetic variation in ETV6 is associated with colorectal cancer susceptibility.

Applications

ChIP

Species

Human

Meilin Wang,Dongying Gu,Mulong Du,Zhi Xu,Suzhan Zhang,Lingjun Zhu,Jiachun Lu,Rui Zhang,Jinliang Xing,Xiaoping Miao,Haiyan Chu,Zhibin Hu,Lei Yang,Cuiju Tang,Lei Pan,Haina Du,Jian Zhao,Jiangbo Du,Na Tong,Jielin Sun,Hongbing Shen,Jianfeng Xu,Zhengdong Zhang,Jinfei Chen

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