Rabbit Polyclonal MAX antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human MAX aa 100 to C-terminus.
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- PloS one 18:e02849492023Validation of Myc-Associated Protein X (MAX) regulation in growth hormone secreting and nonfunctional pituitary adenoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDouglass W Tucker,Dhiraj J Pangal,Robin Du,Angad S Gogia,Ali Tafreshi,Jacob Ruzevick,Kyle T Hurth,Tim Triche,Alexander Micko,John D Carpten,Mark S Shiroishi,John D Carmichael,Suhn K Rhie,Gabriel ZadaPubMed 37104368
- Clinics and practice 12:299-3052022Bilateral Pheochromocytoma with Germline MAX Variant without Family History.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShinnosuke Hata,Mai Asano,Hiroyuki Tominaga,Masahide Hamaguchi,Fumiya Hongo,Takeshi Usui,Eiichi Konishi,Michiaki FukuiPubMed 35645312
- Cancer research communications 2:110-1302022MYC Inhibition Halts Metastatic Breast Cancer Progression by Blocking Growth, Invasion, and Seeding.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Massó-Vallés,Marie-Eve Beaulieu,Toni Jauset,Fabio Giuntini,Mariano F Zacarías-Fluck,Laia Foradada,Sandra Martínez-Martín,Erika Serrano,Génesis Martín-Fernández,Sílvia Casacuberta-Serra,Virginia Castillo Cano,Jastrinjan Kaur,Sergio López-Estévez,Miguel Ángel Morcillo,Mohammad Alzrigat,Loay Mahmoud,Antonio Luque-García,Marta Escorihuela,Marta Guzman,Joaquín Arribas,Violeta Serra,Lars-Gunnar Larsson,Jonathan R Whitfield,Laura SoucekPubMed 36860495
- Endocrine pathology 31:367-3762020Transcription Factor Profiling Identifies Spatially Heterogenous Mediators of Follicular Thyroid Cancer Invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNorman G Nicolson et. al.PubMed 33063251
- Cell chemical biology 28:4-13.e172020Discovery of a Functional Covalent Ligand Targeting an Intrinsically Disordered Cysteine within MYC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLydia Boike,Alexander G Cioffi,Felix C Majewski,Jennifer Co,Nathaniel J Henning,Michael D Jones,Gang Liu,Jeffrey M McKenna,John A Tallarico,Markus Schirle,Daniel K NomuraPubMed 32966806
- Frontiers in endocrinology 11:5582020A Novel Phenotype of Germline Pathogenic Variants in : Concurrence of Pheochromocytoma and Ganglioneuroma in a Chinese Family and Literature Review.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyan Chang et. al.PubMed 32973681
- Scientific reports 10:103912020Decreased MYC-associated factor X (MAX) expression is a new potential biomarker for adverse prognosis in anaplastic large cell lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakahisa Yamashita et. al.PubMed 32587329
- PloS one 12:e01731062017Stabilization of HIF-1α and HIF-2α, up-regulation of MYCC and accumulation of stabilized p53 constitute hallmarks of CNS-PNET animal model.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MAX aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P61244
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Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Platypus | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
| Turkey | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-5 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Orangutan, Platypus, Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Orangutan, Platypus, Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Orangutan, Platypus, Chimpanzee | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements.
Alternative names
BHLHD4, MAX, Protein max, Class D basic helix-loop-helix protein 4, Myc-associated factor X, bHLHd4
Recommended products
Rabbit Polyclonal MAX antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human MAX aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MAX aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P61244
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MAX protein also known as Myc-associated factor X functions mechanically as a transcription factor with a molecular weight of approximately 20 kDa. MAX is expressed in a wide range of tissues throughout the body. The protein forms homodimers or heterodimers with other members of the basic-helix-loop-helix-zipper (bHLHZ) family such as MYC MAD and MNT. The dimerization with different partners determines its role as an activator or repressor of transcription affecting the regulation of various genes.
- Biological function summary
The MAX protein plays a significant role in controlling cell growth differentiation and apoptosis. The protein often forms part of complex regulatory networks within the cell. By interacting with different partner proteins MAX modulates transcriptional activity affecting cellular outcomes. Importantly it can switch between activating or repressing target genes depending on the dimer partner influencing processes such as proliferation and differentiation.
- Pathways
MAX is deeply integrated into the MYC-MAX-MAD pathway an important pathway for regulating gene expression linked to cell cycle and proliferative processes. Within this pathway MAX pairs with either MYC or MAD depending on the cellular context to influence transcription. When paired with MYC it promotes transcription while pairing with MAD results in transcriptional repression. Additionally in the growth signaling pathway the MAX protein’s regulation of target genes is pivotal for ensuring proper cellular responses to growth signals.
- Associated diseases and disorders
The malfunction of the MAX protein has a strong association with cancer and neurodegenerative diseases. Alterations in MAX function can lead to uncontrolled cell growth contributing to oncogenesis particularly in relation to MYC which is a known oncogene. Furthermore dysregulation of the MAX protein is linked to neurodegenerative disorders where abnormal MAX activity may disrupt normal neural gene expression enhancing disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAX antibody (ab101271)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MAX with ab101271 at 1/1000 (1µg/ml).
Western blot - Anti-MAX antibody (ab101271)
All lanes: Western blot - Anti-MAX antibody (ab101271) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 18 kDa
Exposure time: 3min
Immunoprecipitation - Anti-MAX antibody (ab101271)
Detection of MAX in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab101271 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents Immunoprecipitates from control IgG. Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-MAX antibody (ab101271)
Predicted band size: 18 kDa
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