AB227298

Anti-MAX antibody

Name: Anti-MAX antibody (ab227298) | Abcam
Brand: Abcam Limited
SKU: AB227298
Price: 515 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal MAX antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MAX.

View Alternative Names

BHLHD4, MAX, Protein max, Class D basic helix-loop-helix protein 4, Myc-associated factor X, bHLHd4

2 Images

Supplier Data

Immunoprecipitation - Anti-MAX antibody (AB227298)

MAX was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 2 μg of ab227298. Western blot was performed from the immunoprecipitate using ab227298 at 1/1000 dilution. Anti-Rabbit IgG was used as secondary antibody.

Lane 1 : preimmune Rabbit IgG instead of ab227298 in HEK-293T whole cell extract.
Lane 2 : ab227298 IP in HEK-293T whole cell extract.

All lanes:

Immunoprecipitation - Anti-MAX antibody (ab227298)

Predicted band size: 18 kDa

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Supplier Data

Western blot - Anti-MAX antibody (AB227298)

15% SDS-PAGE gel

All lanes:

Western blot - Anti-MAX antibody (ab227298) at 1/500 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg

Lane 2:

SK-N-SH (human neuroblastoma cell line) whole cell extract at 30 µg

Lane 3:

IMR32 (human brain neuroblast cell line) whole cell extract at 30 µg

Lane 4:

SK-N-AS (human brain neuroblastoma cell line) whole cell extract at 30 µg

Predicted band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human MAX. The exact immunogen used to generate this antibody is proprietary information.

P61244

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MAX protein also known as Myc-associated factor X functions mechanically as a transcription factor with a molecular weight of approximately 20 kDa. MAX is expressed in a wide range of tissues throughout the body. The protein forms homodimers or heterodimers with other members of the basic-helix-loop-helix-zipper (bHLHZ) family such as MYC MAD and MNT. The dimerization with different partners determines its role as an activator or repressor of transcription affecting the regulation of various genes.

Biological function summary

The MAX protein plays a significant role in controlling cell growth differentiation and apoptosis. The protein often forms part of complex regulatory networks within the cell. By interacting with different partner proteins MAX modulates transcriptional activity affecting cellular outcomes. Importantly it can switch between activating or repressing target genes depending on the dimer partner influencing processes such as proliferation and differentiation.

Pathways

MAX is deeply integrated into the MYC-MAX-MAD pathway an important pathway for regulating gene expression linked to cell cycle and proliferative processes. Within this pathway MAX pairs with either MYC or MAD depending on the cellular context to influence transcription. When paired with MYC it promotes transcription while pairing with MAD results in transcriptional repression. Additionally in the growth signaling pathway the MAX protein’s regulation of target genes is pivotal for ensuring proper cellular responses to growth signals.

The malfunction of the MAX protein has a strong association with cancer and neurodegenerative diseases. Alterations in MAX function can lead to uncontrolled cell growth contributing to oncogenesis particularly in relation to MYC which is a known oncogene. Furthermore dysregulation of the MAX protein is linked to neurodegenerative disorders where abnormal MAX activity may disrupt normal neural gene expression enhancing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC : MAX complex is a transcriptional activator, whereas the MAD : MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements.

See full target information MAX

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Diabetes, metabolic syndrome and obesity : targets and therapy 18:217-231 PubMed39896707

2025

PEDF Overexpression Ameliorates Cardiac Lipotoxicity in Diabetic Cardiomyopathy via Regulation of Energy Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Tuohua Mao,Ye Wang

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