Anti-Maxi Potassium channel alpha/SLO antibody [L6/60]

• WB

Lab

Western blot - Anti-Maxi Potassium channel alpha/SLO antibody [L6/60] (AB192759)

All lanes:

Western blot - Anti-Maxi Potassium channel alpha/SLO antibody [L6/60] (ab192759) at 5 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/50000 dilution

Predicted band size: 137 kDa

Observed band size: 110 kDa,28 kDa

true

Exposure time: 20min

• IHC-P

Ap2133079****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Maxi Potassium channel alpha/SLO antibody [L6/60] (AB192759)

IHC image of Maxi Potassium channel alpha/SLO antibody [L6/60] staining in rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab192759, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.