Rabbit Polyclonal MAZ antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MAZ aa 400 to C-terminus.
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Publications
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- Frontiers in oncology 11:7667902021SIPL1, Regulated by MAZ, Promotes Tumor Progression and Predicts Poor Survival in Human Triple-Negative Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuanjuan He,Jing Wang,Teng Li,Kunlun Chen,Songchao Li,Shaojin ZhangPubMed 34976812
- EMBO molecular medicine 11:e108352019Therapeutic targeting of circ-CUX1/EWSR1/MAZ axis inhibits glycolysis and neuroblastoma progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuanhuan Li et. al.PubMed 31709724
- Molecular cancer research : MCR 11:161-722012MYC-induced epigenetic activation of GATA4 in lung adenocarcinoma.Applications:Unspecified applicationReactive species:HumanInês C Castro,Achim Breiling,Katharina Luetkenhaus,Fatih Ceteci,Simone Hausmann,Sebastian Kress,Frank Lyko,Thomas Rudel,Ulf R RappPubMed 23239811
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MAZ aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P56270
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Guinea pig, Pig, Chimpanzee, Rhesus monkey, Chinese hamster | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Guinea pig, Pig, Chimpanzee, Rhesus monkey, Chinese hamster | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Guinea pig, Pig, Chimpanzee, Rhesus monkey, Chinese hamster | Dilution info - | Notes - |
Target data
Function
Transcriptional regulator, potentially with dual roles in transcription initiation and termination. Isoform 1. Binds DNA and functions as a transcriptional activator (PubMed:12270922). Binds to two G/A-rich sites, ME1a1 and ME1a2, within the MYC promoter having greater affinity for the former (PubMed:1502157). Also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors (PubMed:1502157). Isoform 2. Binds DNA and functions as a transcriptional activator (PubMed:12270922). Inhibits MAZ isoform 1-mediated transcription (PubMed:12270922). Isoform 3. Binds DNA and functions as a transcriptional activator.
Alternative names
ZNF801, MAZ, Myc-associated zinc finger protein, MAZI, Pur-1, Purine-binding transcription factor, Serum amyloid A-activating factor-1, Transcription factor Zif87, ZF87, Zinc finger protein 801, SAF-1
Recommended products
Rabbit Polyclonal MAZ antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MAZ aa 400 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MAZ aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P56270
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MAZ protein also known as Myc-associated zinc finger protein or Pur-1 has a mass of about 49 kDa. This protein is a transcription factor and contains zinc-finger motifs that enable it to bind to specific DNA sequences influencing gene expression. MAZ is expressed in various tissues including the brain and the heart and plays a significant role in regulating various physiological processes. Researchers often study MAZ for its involvement in cellular proliferation and differentiation.
- Biological function summary
MAZ influences the transcription of genes involved in cell growth and division. It does not function as part of a larger complex but acts by binding directly to DNA influencing the transcription machinery. Its activity is critical in controlling genes linked to cell cycle progression and apoptosis. Its regulatory functions help maintain normal cellular function and tissue homeostasis.
- Pathways
MAZ is integral to the regulation of the MAPK and Wnt signaling pathways. The protein interacts with other signaling molecules in these pathways to modulate gene expression linked to cell proliferation and differentiation. Additionally MAZ has associations with the MYC oncogene highlighting its involvement in pathways related to cellular growth and cancer development.
- Associated diseases and disorders
Researchers find a relationship between MAZ and cancer especially glioblastoma and colorectal cancer. The protein's overexpression can contribute to oncogenic transformation and tumor progression. MAZ also connects with other proteins such as MYC in these disorders highlighting its important role in cancer-related pathways. Understanding the role of MAZ in these diseases could provide insights into therapeutic targets for limiting tumor growth.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAZ antibody (ab85725)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling MAZ with ab85725 at 1/1000 (1μg/mL). Detection: DAB.
Immunoprecipitation - Anti-MAZ antibody (ab85725)
Detection of Human MAZ by Immunoprecipitation in HeLa cell lysate (1 mg for IP, 20% of IP loaded) using ab85725 at 3 ug/mg lysate from HeLa cells (lane 1). Lane 2 is Control IgG IP. Subsequent WB detection was performed using 1 ug/ml ab85725. Detection:
Chemiluminescence with exposure times of 30 seconds.All lanes: Immunoprecipitation - Anti-MAZ antibody (ab85725)
Predicted band size: 49 kDa
Western blot - Anti-MAZ antibody (ab85725)
All lanes: Western blot - Anti-MAZ antibody (ab85725) at 0.1 µg/mL
Lane 1: HeLa cells lysate at 50 µg
Lane 2: HeLa cells lysate at 15 µg
Lane 3: HeLa cells lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 35 kDa, 39 kDa, 60 kDa
Exposure time: 30s
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