JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB247676

Anti-MBD1 antibody [EPR3564] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal MBD1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.

View Alternative Names

CXXC3, PCM1, MBD1, Methyl-CpG-binding domain protein 1, CXXC-type zinc finger protein 3, Methyl-CpG-binding protein MBD1, Protein containing methyl-CpG-binding domain 1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD1 antibody [EPR3564] - BSA and Azide free (AB247676)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD1 antibody [EPR3564] - BSA and Azide free (AB247676)

This data was developed using ab108510, the same antibody clone in a different buffer formulation.

ab108510, at 1/100 dilution, staining MBD1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MBD1 antibody [EPR3564] - BSA and Azide free (AB247676)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MBD1 antibody [EPR3564] - BSA and Azide free (AB247676)

This data was developed using ab108510, the same antibody clone in a different buffer formulation.ab108510, at 1/100 dilution, staining MBD1 in MCF7 cells by Immunofluorescence.

Western blot - Anti-MBD1 antibody [EPR3564] - BSA and Azide free (AB247676)
  • WB

Lab

Western blot - Anti-MBD1 antibody [EPR3564] - BSA and Azide free (AB247676)

This data was developed using the same antibody clone in a different buffer formulation (ab108510).

Lanes 1 - 4 : Merged signal (red and green). Green - ab108510 observed at 70-80 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab108510 was shown to react with MBD1 in wild-type HAP1 cells in western blot with loss of signal observed in MBD1 knockout sample. Wild-type and MBD1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108510 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MBD1 antibody [EPR3564] (<a href='/en-us/products/primary-antibodies/mbd1-antibody-epr3564-ab108510'>ab108510</a>) at 1/500 dilution

Lane 1:

Wild-type HAP1 cell lysate at 40 µg

Lane 2:

MBD1 knockout HAP1 cell lysate at 40 µg

Lane 3:

A431 cell lysate at 40 µg

Lane 4:

Jurkat cell lysate at 40 µg

Predicted band size: 67 kDa

Observed band size: 70-80 kDa

false

Western blot - Anti-MBD1 antibody [EPR3564] - BSA and Azide free (AB247676)
  • WB

Unknown

Western blot - Anti-MBD1 antibody [EPR3564] - BSA and Azide free (AB247676)

This data was developed using ab108510, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MBD1 antibody [EPR3564] (<a href='/en-us/products/primary-antibodies/mbd1-antibody-epr3564-ab108510'>ab108510</a>) at 1/500 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3564

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB188474

Anti-MBD2 antibody [EPR18361]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (AB188474)

  • 0
  • 0
Primary Antibodies

AB253197

Anti-MeCP2 antibody [EPR23201-3]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-MeCP2 antibody [EPR23201-3] (AB253197)

  • 4
  • 4
Primary Antibodies

AB16048

Anti-Lamin B1 antibody - Nuclear Envelope Marker

BOND RX™ Validated|KO Validated|Lab Essentials

Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB16048)

  • 5
  • 110
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab247676 is the carrier-free version of ab108510.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Methyl-CpG binding domain protein 1 (MBD1) also known as CXXC-type and BYE1 domain containing 1 recognizes and binds to methylated CpG sites in the DNA. This protein has a molecular weight of approximately 70 kDa. MBD1 is expressed widely in human tissues with higher levels detectable in the brain particularly the cerebral cortex. It acts within the nucleus where it functions as an important participant in the regulation of gene expression through DNA methylation.
Biological function summary

MBD1 influences the repression or activation of genomic transcription. It binds to methylated DNA and interacts with other proteins including methyl CpG binding proteins to form transcriptional repression complexes. This activity plays a role in gene silencing and modulation. MBD1 ensures proper interpretation of epigenetic marks therefore sustaining cellular identity and lineage commitment. In some cells MBD1 also engages with chromatin remodeling complexes to influence chromatin structure.

Pathways

MBD1 plays a significant role in the epigenetic and transcription regulation pathways. It is directly involved in DNA methylation processes interacting with histone deacetylases such as HDAC1 and other chromatin-modifying proteins. The pathway provides an important mechanism for long-term transcriptional silencing affecting developmental processes and cellular differentiation through methylation-dependent transcriptional regulation. MBD1 shares pathway interactions with proteins like MeCP2 highlighting interconnected roles in maintaining DNA methylation landscapes.

MBD1 has links to neurological disorders and cancer. It shows associations with Rett syndrome a neurodevelopmental disorder through its interaction with other methyl CpG binding proteins. Mutations or dysregulation in MBD1’s function can disrupt normal methylation patterns contributing to gene expression changes seen in disease states. In cancer aberrant MBD1 expression may lead to inappropriate gene silencing impacting tumor suppressor genes. Many cancers including glioblastoma exhibit altered MBD1 expression levels implicating its role in tumorigenesis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
See full target information MBD1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com