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AB188474

Anti-MBD2 antibody [EPR18361]

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(10 Publications)

Rabbit Recombinant Monoclonal MBD2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

View Alternative Names

Methyl-CpG-binding domain protein 2, Demethylase, Methyl-CpG-binding protein MBD2, DMTase, MBD2

11 Images
Flow Cytometry (Intracellular) - Anti-MBD2 antibody [EPR18361] (AB188474)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MBD2 antibody [EPR18361] (AB188474)

Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling MBD2 with purified ab188474 at 1/70 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunocytochemistry/ Immunofluorescence - Anti-MBD2 antibody [EPR18361] (AB188474)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MBD2 antibody [EPR18361] (AB188474)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling MBD2 with ab188474 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse (AlexaFluor®594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab188474 at 1/250 dilution, followed by Goat Anti-Mouse (AlexaFluor®594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (AB188474)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (AB188474)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MBD2 with ab188474 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on human colon tissue tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (AB188474)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (AB188474)

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling MBD2 with ab188474 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on human gastric cancer tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-MBD2 antibody [EPR18361] (AB188474)
  • IP

Supplier Data

Immunoprecipitation - Anti-MBD2 antibody [EPR18361] (AB188474)

MBD2 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab188474 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab188474 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.
Lane 1 : HeLa whole cell lysate 10μg (Input).
Lane 2 : ab188474 IP in HeLa whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab188474 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-MBD2 antibody [EPR18361] (ab188474)

Predicted band size: 43 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (AB188474)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (AB188474)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling MBD2 with ab188474 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on rat colon tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (AB188474)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (AB188474)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling MBD2 with ab188474 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on mouse stomach tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-MBD2 antibody [EPR18361] (AB188474)
  • WB

Lab

Western blot - Anti-MBD2 antibody [EPR18361] (AB188474)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : MBD2 knockout HAP1 cell lysate (20 μg)
Lane 3 : A375 cell lysate (20 μg)
Lane 4 : Human brain tissue lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab188474 observed at 32 & 49 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab188474 was shown to specifically react with MBD2 when MBD2 knockout samples were used. Wild-type and MBD2 knockout samples were subjected to SDS-PAGE. ab188474 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ( ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-MBD2 antibody [EPR18361] (ab188474)

Predicted band size: 43 kDa

false

Western blot - Anti-MBD2 antibody [EPR18361] (AB188474)
  • WB

Supplier Data

Western blot - Anti-MBD2 antibody [EPR18361] (AB188474)

Blocking/Dilution buffer : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID : 17353267).

All lanes:

Western blot - Anti-MBD2 antibody [EPR18361] (ab188474) at 1/10000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 20 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast cell line) lysate at 20 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 43 kDa

Observed band size: 29 kDa,43 kDa

false

Exposure time: 15s

Western blot - Anti-MBD2 antibody [EPR18361] (AB188474)
  • WB

Supplier Data

Western blot - Anti-MBD2 antibody [EPR18361] (AB188474)

5% NFDM/TBST : Blocking and dilution buffer.

The observed MW is consistent with what has been described in the literature (PMID : 17353267).

All lanes:

Western blot - Anti-MBD2 antibody [EPR18361] (ab188474) at 1/10000 dilution

Lane 1:

A-375 (Human malignant melanoma cell line) lysate at 20 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma cell line) lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 43 kDa

Observed band size: 29 kDa,43 kDa

false

Exposure time: 3min

Western blot - Anti-MBD2 antibody [EPR18361] (AB188474)
  • WB

Supplier Data

Western blot - Anti-MBD2 antibody [EPR18361] (AB188474)

Blocking/Dilution buffer : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID : 17353267).

All lanes:

Western blot - Anti-MBD2 antibody [EPR18361] (ab188474) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Rat brain lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 43 kDa

Observed band size: 29 kDa,43 kDa

false

Exposure time: 30s

  • Carrier free

    Anti-MBD2 antibody [EPR18361] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18361

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IP, Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MBD2 also known as methyl-CpG-binding domain protein 2 is a protein with a molecular mass of about 45 kDa. This protein binds to methylated DNA specifically at CpG islands and plays a role in gene silencing. MBD2 is expressed in various tissues throughout the body including the brain liver and spleen. It contains a methyl-CpG-binding domain which facilitates its interaction with methylated DNA sequences influencing the transcription of particular genes.
Biological function summary

MBD2 functions as a transcriptional repressor. It is part of the MeCP1 complex which includes proteins such as MTA2 and HDAC1. This complex remodels chromatin structure to suppress gene expression. MBD2's ability to recruit chromatin remodeling enzymes allows it to modulate access to the underlying DNA affecting the gene activity and playing a role in cellular differentiation and development.

Pathways

The role of MBD2 is particularly important in DNA methylation and chromatin modification pathways. It interacts with the DNA methylation machinery influencing epigenetic regulation. MBD2 works closely with other proteins like MeCP2 which shares similar binding properties to control gene expression through epigenetic mechanisms. These interactions highlight its role in modulating cellular function and responding to changes in the cellular environment.

Alterations in MBD2 have been linked to various cancers and neurological disorders. MBD2 deregulation affects pathways involved in tumor suppression and its overexpression has been noted in certain aggressive forms of cancer. In the context of neurological disorders MBD2 together with its related protein MeCP2 is implicated in conditions like Rett syndrome. Understanding the role of MBD2 in these diseases offers potential therapeutic avenues in targeting epigenetic modifications for treatment.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides (PubMed : 9774669). Binds hemimethylated DNA as well (PubMed : 10947852, PubMed : 24307175). Recruits histone deacetylases and DNA methyltransferases to chromatin (PubMed : 10471499, PubMed : 10947852). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 28977666). Acts as a transcriptional repressor and plays a role in gene silencing (PubMed : 10471499, PubMed : 10947852, PubMed : 16415179). Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression (PubMed : 16415179). May enhance the activation of some unmethylated cAMP-responsive promoters (PubMed : 12665568).
See full target information MBD2

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6919 PubMed40715076

2025

Removal of promoter CpG methylation by epigenome editing reverses HBG silencing.

Applications

Unspecified application

Species

Unspecified reactive species

Henry W Bell,Ruopeng Feng,Manan Shah,Yu Yao,James Douglas,Phillip A Doerfler,Thiyagaraj Mayuranathan,Michael F O'Dea,Yichao Li,Yong-Dong Wang,Jingjing Zhang,Joel P Mackay,Yong Cheng,Kate G R Quinlan,Mitchell J Weiss,Merlin Crossley

Cell reports 43:114991 PubMed39570746

2024

ZBTB7A is a modulator of KDM5-driven transcriptional networks in basal breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Benedetto DiCiaccio,Marco Seehawer,Zheqi Li,Andriana Patmanidis,Triet Bui,Pierre Foidart,Jun Nishida,Clive S D'Santos,Evangelia K Papachristou,Malvina Papanastasiou,Andrew H Reiter,Xintao Qiu,Rong Li,Yijia Jiang,Xiao-Yun Huang,Anton Simeonov,Stephen C Kales,Ganesha Rai,Madhu Lal-Nag,Ajit Jadhav,Myles Brown,Jason S Carroll,Henry W Long,Kornelia Polyak

Journal of orthopaedic surgery and research 19:517 PubMed39198853

2024

Novel insights into vancomycin-loaded calcium sulfate and negative pressure wound therapy in preventing infections in open fractures.

Applications

Unspecified application

Species

Unspecified reactive species

Bei Jia,Rui Xue,Jia Li,Jichao Guo,Jianning Liu

The EMBO journal 42:e113448 PubMed37737560

2023

BCL11B and the NuRD complex cooperatively guard T-cell fate and inhibit OPA1-mediated mitochondrial fusion in T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Liao,Yi Wu,Le Qin,Zhiwu Jiang,Shixue Gou,Linfu Zhou,Qilan Hong,Yao Li,Jingxuan Shi,Yao Yao,Liangxue Lai,Yangqiu Li,Pentao Liu,Jean Paul Thiery,Dajiang Qin,Thomas Graf,Xingguo Liu,Peng Li

Nature communications 14:3848 PubMed37385984

2023

Dissecting the roles of MBD2 isoforms and domains in regulating NuRD complex function during cellular differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Schmolka,Ino D Karemaker,Richard Cardoso da Silva,Davide C Recchia,Vincent Spegg,Jahnavi Bhaskaran,Michael Teske,Nathalie P de Wagenaar,Matthias Altmeyer,Tuncay Baubec

International journal of oncology 61: PubMed35904180

2022

lncKRT16P6 promotes tongue squamous cell carcinoma progression by sponging miR‑3180 and regulating GATAD2A expression.

Applications

Unspecified application

Species

Unspecified reactive species

Mi Zhang,Ling Wu,Xudong Wang,Jiang Chen

Molecular therapy. Nucleic acids 26:1201-1214 PubMed34853720

2021

Mbd2 deficiency alleviates retinal cell apoptosisvia the miR-345-5p/Atf1 axis in high glucoseinjury and streptozotocin-induced diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yanni Ge,Ran Zhang,Yuqing Feng,Jinfang Lu,Huiling Li

Cell death and differentiation 29:218-229 PubMed34420035

2021

MBD2 acts as a repressor to maintain the homeostasis of the Th1 program in type 1 diabetes by regulating the STAT1-IFN-γ axis.

Applications

Unspecified application

Species

Unspecified reactive species

Tiantian Yue,Fei Sun,Faxi Wang,Chunliang Yang,Jiahui Luo,Shanjie Rong,Haifeng Zhou,Jun Xiao,Xiaohui Wang,Qing Zhou,Ping Yang,Shu Zhang,Wen Li,Fei Xiong,Qilin Yu,Cong-Yi Wang

Oncology letters 20:2749-2756 PubMed32782591

2020

Expression and clinical significance of methyl-CpG binding domain protein 2 in high-grade serous ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wangang Gong,Maowei Ni,Zhongbo Chen,Zhiguo Zheng

Molecular therapy. Nucleic acids 19:1250-1265 PubMed32074940

2020

Mbd2 Mediates Retinal Cell Apoptosis by Targeting the lncRNA Mbd2-AL1/miR-188-3p/Traf3 Axis in Ischemia/Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yanni Ge,Ran Zhang,Yuqing Feng,Huiling Li
View all publications

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