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Rabbit Recombinant Monoclonal MBD2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

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Images

Western blot - Anti-MBD2 antibody [EPR18361] (AB188474), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MBD2 antibody [EPR18361] (AB188474), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (AB188474), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MBD2 antibody [EPR18361] (AB188474), expandable thumbnail
  • Western blot - Anti-MBD2 antibody [EPR18361] (AB188474), expandable thumbnail

Publications

  • The EMBO journal 42:e1134482023
    BCL11B and the NuRD complex cooperatively guard T-cell fate and inhibit OPA1-mediated mitochondrial fusion in T cells.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Rui Liao,Yi Wu,Le Qin,Zhiwu Jiang,Shixue Gou,Linfu Zhou,Qilan Hong,Yao Li,Jingxuan Shi,Yao Yao,Liangxue Lai,Yangqiu Li,Pentao Liu,Jean Paul Thiery,Dajiang Qin,Thomas Graf,Xingguo Liu,Peng Li
    PubMed 37737560
  • Nature communications 14:38482023
    Dissecting the roles of MBD2 isoforms and domains in regulating NuRD complex function during cellular differentiation.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Nina Schmolka,Ino D Karemaker,Richard Cardoso da Silva,Davide C Recchia,Vincent Spegg,Jahnavi Bhaskaran,Michael Teske,Nathalie P de Wagenaar,Matthias Altmeyer,Tuncay Baubec
    PubMed 37385984

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Tested
Rat
Expected
Tested
Expected
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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      Target data

      Function

      Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.

      Alternative names

      Methyl-CpG-binding domain protein 2, Demethylase, Methyl-CpG-binding protein MBD2, DMTase, MBD2

      Recommended products

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      Rabbit Recombinant Monoclonal MBD2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

      Alternative names

      Methyl-CpG-binding domain protein 2, Demethylase, Methyl-CpG-binding protein MBD2, DMTase, MBD2

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR18361

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Supplementary info

      Activity summary

      MBD2 also known as methyl-CpG-binding domain protein 2 is a protein with a molecular mass of about 45 kDa. This protein binds to methylated DNA specifically at CpG islands and plays a role in gene silencing. MBD2 is expressed in various tissues throughout the body including the brain liver and spleen. It contains a methyl-CpG-binding domain which facilitates its interaction with methylated DNA sequences influencing the transcription of particular genes.

      Biological function summary

      MBD2 functions as a transcriptional repressor. It is part of the MeCP1 complex which includes proteins such as MTA2 and HDAC1. This complex remodels chromatin structure to suppress gene expression. MBD2's ability to recruit chromatin remodeling enzymes allows it to modulate access to the underlying DNA affecting the gene activity and playing a role in cellular differentiation and development.

      Pathways

      The role of MBD2 is particularly important in DNA methylation and chromatin modification pathways. It interacts with the DNA methylation machinery influencing epigenetic regulation. MBD2 works closely with other proteins like MeCP2 which shares similar binding properties to control gene expression through epigenetic mechanisms. These interactions highlight its role in modulating cellular function and responding to changes in the cellular environment.

      Associated diseases and disorders

      Alterations in MBD2 have been linked to various cancers and neurological disorders. MBD2 deregulation affects pathways involved in tumor suppression and its overexpression has been noted in certain aggressive forms of cancer. In the context of neurological disorders MBD2 together with its related protein MeCP2 is implicated in conditions like Rett syndrome. Understanding the role of MBD2 in these diseases offers potential therapeutic avenues in targeting epigenetic modifications for treatment.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      11 product images

      • Western blot - Anti-MBD2 antibody [EPR18361] (ab188474), expandable thumbnail

        Western blot - Anti-MBD2 antibody [EPR18361] (ab188474)

        Lane 1: Wild-type HAP1 cell lysate (20 μg)
        Lane 2: MBD2 knockout HAP1 cell lysate (20 μg)
        Lane 3: A375 cell lysate (20 μg)
        Lane 4: Human brain tissue lysate (20 μg)
        Lanes 1 - 4: Merged signal (red and green). Green - ab188474 observed at 32 & 49 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
        ab188474 was shown to specifically react with MBD2 when MBD2 knockout samples were used. Wild-type and MBD2 knockout samples were subjected to SDS-PAGE. ab188474 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ( Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

        All lanes: Western blot - Anti-MBD2 antibody [EPR18361] (ab188474)

        Predicted band size: 43 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-MBD2 antibody [EPR18361] (ab188474), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-MBD2 antibody [EPR18361] (ab188474)

        Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling MBD2 with ab188474 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).

        Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse (AlexaFluor®594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red).

        The negative controls are as follows:
        -ve control 1: ab188474 at 1/250 dilution, followed by Goat Anti-Mouse (AlexaFluor®594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
        -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (ab188474), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (ab188474)

        Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling MBD2 with ab188474 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on rat colon tissue is observed. Counter stained with Hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Flow Cytometry (Intracellular) - Anti-MBD2 antibody [EPR18361] (ab188474), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-MBD2 antibody [EPR18361] (ab188474)

        Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling MBD2 with purified ab188474 at 1/70 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

      • Western blot - Anti-MBD2 antibody [EPR18361] (ab188474), expandable thumbnail

        Western blot - Anti-MBD2 antibody [EPR18361] (ab188474)

        Blocking/Dilution buffer: 5% NFDM/TBST.

        The observed MW is consistent with what has been described in the literature (PMID: 17353267).

        All lanes: Western blot - Anti-MBD2 antibody [EPR18361] (ab188474) at 1/10000 dilution

        Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 20 µg

        Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) lysate at 20 µg

        Lane 3: MCF7 (Human breast adenocarcinoma cell line) lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

        Predicted band size: 43 kDa

        Observed band size: 29 kDa, 43 kDa

        Exposure time: 15s

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (ab188474), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (ab188474)

        Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MBD2 with ab188474 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on human colon tissue tissue is observed. Counter stained with Hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Western blot - Anti-MBD2 antibody [EPR18361] (ab188474), expandable thumbnail

        Western blot - Anti-MBD2 antibody [EPR18361] (ab188474)

        5% NFDM/TBST: Blocking and dilution buffer.

        The observed MW is consistent with what has been described in the literature (PMID:17353267).

        All lanes: Western blot - Anti-MBD2 antibody [EPR18361] (ab188474) at 1/10000 dilution

        Lane 1: A-375 (Human malignant melanoma cell line) lysate at 20 µg

        Lane 2: PC-12 (Rat adrenal gland pheochromocytoma cell line) lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

        Predicted band size: 43 kDa

        Observed band size: 29 kDa, 43 kDa

        Exposure time: 3min

      • Immunoprecipitation - Anti-MBD2 antibody [EPR18361] (ab188474), expandable thumbnail

        Immunoprecipitation - Anti-MBD2 antibody [EPR18361] (ab188474)

        MBD2 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab188474 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab188474 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.
        Lane 1: HeLa whole cell lysate 10μg (Input).
        Lane 2: ab188474 IP in HeLa whole cell lysate.
        Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab188474 in HeLa whole cell lysate.
        Blocking and dilution buffer and concentration: 5% NFDM/TBST.
        Exposure time: 3 seconds.

        All lanes: Immunoprecipitation - Anti-MBD2 antibody [EPR18361] (ab188474)

        Predicted band size: 43 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (ab188474), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (ab188474)

        Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling MBD2 with ab188474 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on human gastric cancer tissue is observed. Counter stained with Hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Western blot - Anti-MBD2 antibody [EPR18361] (ab188474), expandable thumbnail

        Western blot - Anti-MBD2 antibody [EPR18361] (ab188474)

        Blocking/Dilution buffer: 5% NFDM/TBST.

        The observed MW is consistent with what has been described in the literature (PMID:17353267).

        All lanes: Western blot - Anti-MBD2 antibody [EPR18361] (ab188474) at 1/1000 dilution

        Lane 1: Mouse brain lysate at 10 µg

        Lane 2: Mouse heart lysate at 10 µg

        Lane 3: Rat brain lysate at 10 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

        Predicted band size: 43 kDa

        Observed band size: 29 kDa, 43 kDa

        Exposure time: 30s

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (ab188474), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (ab188474)

        Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling MBD2 with ab188474 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on mouse stomach tissue is observed. Counter stained with Hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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