Anti-MBD2 antibody [EPR18361]
- RabMAb
- Recombinant
- KO Validated
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(10 Publications)
Rabbit Recombinant Monoclonal MBD2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 10 publications.
View Alternative Names
Methyl-CpG-binding domain protein 2, Demethylase, Methyl-CpG-binding protein MBD2, DMTase, MBD2
- Flow Cyt (Intra)
Lab
Flow Cytometry (Intracellular) - Anti-MBD2 antibody [EPR18361] (AB188474)
Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling MBD2 with purified ab188474 at 1/70 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-MBD2 antibody [EPR18361] (AB188474)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling MBD2 with ab188474 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse (AlexaFluor®594) (ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows :
-ve control 1 : ab188474 at 1/250 dilution, followed by Goat Anti-Mouse (AlexaFluor®594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (AB188474)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MBD2 with ab188474 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on human colon tissue tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (AB188474)
Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling MBD2 with ab188474 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on human gastric cancer tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IP
Supplier Data
Immunoprecipitation - Anti-MBD2 antibody [EPR18361] (AB188474)
MBD2 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab188474 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab188474 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.
Lane 1 : HeLa whole cell lysate 10μg (Input).
Lane 2 : ab188474 IP in HeLa whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab188474 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 seconds.
All lanes:
Immunoprecipitation - Anti-MBD2 antibody [EPR18361] (ab188474)
Predicted band size: 43 kDa
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (AB188474)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling MBD2 with ab188474 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on rat colon tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody [EPR18361] (AB188474)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling MBD2 with ab188474 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on mouse stomach tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Lab
Western blot - Anti-MBD2 antibody [EPR18361] (AB188474)
Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : MBD2 knockout HAP1 cell lysate (20 μg)
Lane 3 : A375 cell lysate (20 μg)
Lane 4 : Human brain tissue lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab188474 observed at 32 & 49 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab188474 was shown to specifically react with MBD2 when MBD2 knockout samples were used. Wild-type and MBD2 knockout samples were subjected to SDS-PAGE. ab188474 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ( ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-MBD2 antibody [EPR18361] (ab188474)
Predicted band size: 43 kDa
false
- WB
Supplier Data
Western blot - Anti-MBD2 antibody [EPR18361] (AB188474)
Blocking/Dilution buffer : 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID : 17353267).
All lanes:
Western blot - Anti-MBD2 antibody [EPR18361] (ab188474) at 1/10000 dilution
Lane 1:
HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 20 µg
Lane 2:
NIH/3T3 (Mouse embryonic fibroblast cell line) lysate at 20 µg
Lane 3:
MCF7 (Human breast adenocarcinoma cell line) lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 29 kDa,43 kDa
false
Exposure time: 15s
- WB
Supplier Data
Western blot - Anti-MBD2 antibody [EPR18361] (AB188474)
5% NFDM/TBST : Blocking and dilution buffer.
The observed MW is consistent with what has been described in the literature (PMID : 17353267).
All lanes:
Western blot - Anti-MBD2 antibody [EPR18361] (ab188474) at 1/10000 dilution
Lane 1:
A-375 (Human malignant melanoma cell line) lysate at 20 µg
Lane 2:
PC-12 (Rat adrenal gland pheochromocytoma cell line) lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 29 kDa,43 kDa
false
Exposure time: 3min
- WB
Supplier Data
Western blot - Anti-MBD2 antibody [EPR18361] (AB188474)
Blocking/Dilution buffer : 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID : 17353267).
All lanes:
Western blot - Anti-MBD2 antibody [EPR18361] (ab188474) at 1/1000 dilution
Lane 1:
Mouse brain lysate at 10 µg
Lane 2:
Mouse heart lysate at 10 µg
Lane 3:
Rat brain lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 29 kDa,43 kDa
false
Exposure time: 30s
Related conjugates and formulations (1)
-
Anti-MBD2 antibody [EPR18361] - BSA and Azide free
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MBD2 functions as a transcriptional repressor. It is part of the MeCP1 complex which includes proteins such as MTA2 and HDAC1. This complex remodels chromatin structure to suppress gene expression. MBD2's ability to recruit chromatin remodeling enzymes allows it to modulate access to the underlying DNA affecting the gene activity and playing a role in cellular differentiation and development.
Pathways
The role of MBD2 is particularly important in DNA methylation and chromatin modification pathways. It interacts with the DNA methylation machinery influencing epigenetic regulation. MBD2 works closely with other proteins like MeCP2 which shares similar binding properties to control gene expression through epigenetic mechanisms. These interactions highlight its role in modulating cellular function and responding to changes in the cellular environment.
Product protocols
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Target data
Publications (10)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 16:6919 PubMed40715076
2025
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Cell reports 43:114991 PubMed39570746
2024
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Journal of orthopaedic surgery and research 19:517 PubMed39198853
2024
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The EMBO journal 42:e113448 PubMed37737560
2023
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Nature communications 14:3848 PubMed37385984
2023
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International journal of oncology 61: PubMed35904180
2022
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Molecular therapy. Nucleic acids 26:1201-1214 PubMed34853720
2021
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Cell death and differentiation 29:218-229 PubMed34420035
2021
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Oncology letters 20:2749-2756 PubMed32782591
2020
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Molecular therapy. Nucleic acids 19:1250-1265 PubMed32074940
2020
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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